Track-by-Day: A standardized approach to estrous cycle monitoring in biobehavioral research

Raimondi, Gianna M.; Eng, Ashley K.; Kenny, Murphy P.; Britting, Madison A.; Ostroff, Linnaea E.

doi:10.1016/j.bbr.2024.114860

Cited by 3 publications

(1 citation statement)

References 71 publications

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“…The washes were stained with methylene blue (5 mg/mL) and analyzed by optical microscopy. The phases of the estrous cycle were identified according to the morphological characteristics of the cells, as follows: (a) DIE/metestrus-few nucleated and cornified cells and many leucocytes; metestrus and DIE (or DIE I and II) are both considered the phases of the EST cycle where steroid hormones are low and with similar vaginal cytology (Caligioni, 2009); (b) PRO-abundant nucleated epithelial cells; and (c) EST-abundant cornified cells (Raimondi et al, 2024). The hormonal profile throughout the estrous cycle comprises low levels of estradiol and PROG during DIE, a transient increase in estradiol during PRO, followed by a transient increase in PROG levels during the EST (Lovick & Zangrossi, 2021).…”

Section: Estrous Cycle Determinationmentioning

confidence: 99%

Diverse modulatory effects of sex steroids on depressive-like behavior in socially stressed female rats.

do Nascimento,

Dierschnabel,

Queiroz

et al. 2024

Psychology & Neuroscience

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The role of sex steroids in mammals extends beyond the conventional influence on the reproductive system. Sex hormones have been shown to modulate Public Significance StatementHormones connected to the hypothalamic-pituitary-adrenal and the hypothalamicpituitary-gonadal axes can interact and influence affective-like behaviors. However, the neurological basis of the role of these hormones in depressive illnesses caused by chronic stress is still debated, in part due to a lack of studies that include females in their experimental protocols. This study suggests that endogenous fluctuations in estrogen levels across the estrous cycle affect depressive-like behaviors triggered by social isolation. Estradiol had a beneficial effect on depressive-like behaviors, which was counteracted by prior exogenous administration of the antagonist tamoxifen. Furthermore, neither progesterone nor mifepristone caused significant changes.

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Section: Estrous Cycle Determinationmentioning

confidence: 99%

Diverse modulatory effects of sex steroids on depressive-like behavior in socially stressed female rats.

do Nascimento,

Dierschnabel,

Queiroz

et al. 2024

Psychology & Neuroscience

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The impact of estrous cycle on anxiety-like behaviour during unlearned fear tests in female rats and mice: A systematic review and meta-analysis

Pestana,

Graham

2024

Neuroscience & Biobehavioral Reviews

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Antiepileptic and Neuroprotective Effects of Rheum tanguticum Root Extract on Trimethyltin-Induced Epilepsy and Neurodegeneration: In Vivo and in Silico Analyses

Choi,

Kang,

Tran

et al. 2024

J. Integr. Neurosci.

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Background: Rheum tanguticum root, cataloged as “Daehwang” in the Korean Pharmacopeia, is rich in various anthraquinones known for their anti-inflammatory and antioxidant properties. Formulations containing Daehwang are traditionally employed for treating neurological conditions. This study aimed to substantiate the antiepileptic and neuroprotective efficacy of R. tanguticum root extract (RTE) against trimethyltin (TMT)-induced epileptic seizures and hippocampal neurodegeneration. Methods: The constituents of RTE were identified by ultra-performance liquid chromatography (UPLC). Experimental animals were grouped into the following five categories: control, TMT, and three TMT+RTE groups with dosages of 10, 30, and 100 mg/kg. Seizure severity was assessed daily for comparison between the groups. Brain tissue samples were examined to determine the extent of neurodegeneration and neuroinflammation using histological and molecular biology techniques. Network pharmacology analysis involved extracting herbal targets for Daehwang and disease targets for epilepsy from multiple databases. A protein-protein interaction network was built using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database, and pivotal targets were determined by topological analysis. Enrichment analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool to elucidate the underlying mechanisms. Results: The RTE formulation was found to contain sennoside A, sennoside B, chrysophanol, emodin, physcion, (+)-catechin, and quercetin-3-O-glucuronoid. RTE effectively inhibited TMT-induced seizures at 10, 30, and 100 mg/kg dosages and attenuated hippocampal neuronal decay and neuroinflammation at 30 and 100 mg/kg dosages. Furthermore, RTE significantly reduced mRNA levels of tumor necrosis factor (TNF-α), glial fibrillary acidic protein (GFAP), and c-fos in hippocampal tissues. Network analysis revealed TNF, Interleukin-1 beta (IL-1β), Interleukin-6 (IL-6), Protein c-fos (FOS), RAC-alpha serine/threonine-protein kinase (AKT1), and Mammalian target of rapamycin (mTOR) as the core targets. Enrichment analysis demonstrated significant involvement of R. tanguticum components in neurodegeneration (p = 4.35 × 10-5) and TNF signaling pathway (p = 9.94 × 10-5). Conclusions: The in vivo and in silico analyses performed in this study suggests that RTE can potentially modulate TMT-induced epileptic seizures and neurodegeneration. Therefore, R. tanguticum root is a promising herbal treatment option for antiepileptic and neuroprotective applications.

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Track-by-Day: A standardized approach to estrous cycle monitoring in biobehavioral research

Cited by 3 publications

References 71 publications

Diverse modulatory effects of sex steroids on depressive-like behavior in socially stressed female rats.

Diverse modulatory effects of sex steroids on depressive-like behavior in socially stressed female rats.

The impact of estrous cycle on anxiety-like behaviour during unlearned fear tests in female rats and mice: A systematic review and meta-analysis

Antiepileptic and Neuroprotective Effects of Rheum tanguticum Root Extract on Trimethyltin-Induced Epilepsy and Neurodegeneration: In Vivo and in Silico Analyses

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