Tracking Intrahepatically Transplanted Islets Labeled With Feridex-Polyethyleneimine Complex Using a Clinical 3.0-T Magnetic Resonance Imaging Scanner

Zhang, Shanzhong; He, Huijin; Lu, Wenwei; Xu, Qinjun; Zhou, Bijing; Tang, Xiaoning

doi:10.1097/mpa.0b013e318190d77a

Cited by 20 publications

(15 citation statements)

References 25 publications

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“…Undoubtedly, this capability is important for longitudinal MR tracking of transplanted IPCs in the liver. In previous studies 23, 27 , the majority of cells in the core region of isolated islets were not labeled with SPIONs, probably due to the difficulty of dispersing SPIONs into the core of the islets. This low level of core labeling might result in a false sharp decrease in the total areas of hypointense spots on MR images even when only peripheral SPION-labeled islet cells are destroyed.…”

Section: Discussionmentioning

confidence: 82%

“…Several groups have demonstrated the feasibility of imaging pancreatic islets by labeling with SPIONs in mouse, rat and monkey models of T1D 12, 18, 27 . However, few studies have considered the temporal relationship between the loss of SPION-labeled IPCs on MRI and the decrease in blood insulin or C-peptide levels, and few have demonstrated that SPION-based MR tracking is more sensitive for timely detection of IPC loss than measuring blood insulin or C-peptide levels.…”

Section: Discussionmentioning

confidence: 99%

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MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys

Zou

Lü

Teng

et al. 2017

Sci Rep

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Insulin-producing cells (IPCs) derived from a patient’s own stem cells offer great potential for autologous transplantation in diabetic patients. However, the limited survival of engrafted cells remains a bottleneck in the application of this strategy. The present study aimed to investigate whether nanoparticle-based magnetic resonance (MR) tracking can be used to detect the loss of grafted stem cell-derived IPCs in a sensitive and timely manner in a diabetic monkey model. Pancreatic progenitor cells (PPCs) were isolated from diabetic monkeys and labeled with superparamagnetic iron oxide nanoparticles (SPIONs). The SPION-labeled cells presented as hypointense signals on MR imaging (MRI). The labeling procedure did not affect the viability or IPC differentiation of PPCs. Importantly, the total area of the hypointense signal caused by SPION-labeled IPCs on liver MRI decreased before the decline in C-peptide levels after autotransplantation. Histological analysis revealed no detectable immune response to the grafts and many surviving insulin- and Prussian blue-positive cell clusters on liver sections at one year post-transplantation. Collectively, this study demonstrates that SPIO nanoparticles can be used to label stem cells for noninvasive, sensitive, longitudinal monitoring of stem cell-derived IPCs in large animal models using a conventional MR imager.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys

Zou

Lü

Teng

et al. 2017

Sci Rep

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“…As shown by Evgenov et al [26], iron distribution within islet cells is not uniform, ranging from 10% to 70%. Recent studies tried to improve efficacy of islet labeling by using liposomes or polyethyleneimine [28,29]. It was shown that isolated human and rodent islets can be labeled with SPIO nanoparticles without impairing their insulin-secreting capacity or viability [24,25,27].…”

Section: Spio Labeling Studiesmentioning

confidence: 99%

Noninvasive Imaging Techniques in Islet Transplantation

et al. 2011

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Since the Edmonton trials, insulin independence can reproducibly be achieved after islet transplantation. However, a majority of patients resume insulin treatment in the first 5 years after transplantation. Several mechanisms have been proposed but are difficult to pinpoint in one particular patient. Current tools for the metabolic monitoring of islet grafts indicate islet dysfunction when it is too late to take action. Noninvasive imaging of transplanted islets could be used to study β-cell mass and β-cell function just after infusion, during vascularization or autoimmune and alloimmune attacks. This review will focus on the most recent advances in various imaging techniques (bioluminescence imaging, fluorescence optical imaging, MRI, and positron emission tomography). Emphasis will be placed on pertinent approaches for translation to human practice.

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“…liposomes >300 nm in diameter) can further hinder uptake and affect islet function and viability. Label uptake can be enhanced using contrast agent with special coatings [68, 69], specific antibodies [70], encapsulation of the agent in specially formulated liposomes [71] or binding to positively-charged peptides loaded by electroporation [72]. Several of these approaches are not easily translatable to the clinic owing to their experimental nature, or financial or logistical difficulties.…”

Section: Mrimentioning

confidence: 99%

Obstacles on the way to the clinical visualisation of beta cells: looking for the Aeneas of molecular imaging to navigate between Scylla and Charybdis

et al. 2012

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For more than a decade, researchers have been trying to develop non-invasive imaging techniques for the in vivo measurement of viable pancreatic beta cells. However, in spite of intense research efforts, only one tracer for positron emission tomography (PET) imaging is currently under clinical evaluation. To many diabetologists it may remain unclear why the imaging world struggles to develop an effective method for non-invasive beta cell imaging (BCI), which could be useful for both research and clinical purposes. Here, we provide a concise overview of the obstacles and challenges encountered on the way to such BCI, in both native and transplanted islets. We discuss the major difficulties posed by the anatomical and cell biological features of pancreatic islets, as well as the chemical and physical limits of the main imaging modalities, with special focus on PET, SPECT and MRI. We conclude by indicating new avenues for future research in the field, based on several remarkable recent results.

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Tracking Intrahepatically Transplanted Islets Labeled With Feridex-Polyethyleneimine Complex Using a Clinical 3.0-T Magnetic Resonance Imaging Scanner

Abstract: Either spot counting or signal intensity measurement provides a perfect quantification of transplanted and surviving islets in vivo. Feridex-PEI provides an effective and safe way to label islets.

Cited by 20 publications

References 25 publications

MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys

MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys

Noninvasive Imaging Techniques in Islet Transplantation

Obstacles on the way to the clinical visualisation of beta cells: looking for the Aeneas of molecular imaging to navigate between Scylla and Charybdis

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