Tracking Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            Clones during a 5-Year Period (1998 to 2002) in a Spanish Hospital

Pérez-Roth, Eduardo; Lorenzo-Díaz, Fabián; Batista, Nínive; Moreno, A. Vives; Méndez-Álvarez, Sebastián

doi:10.1128/jcm.42.10.4649-4656.2004

Cited by 91 publications

(91 citation statements)

References 49 publications

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“…Three strains were included in CC22 (EMRSA-15), a British clone first detected in 1999 in Spain, always detected in a very low percentage (<2%). 6 The tst gene was detected in one isolate (belonging to CC5/ST5/spat9298/SCCmecI/agrII). This strain seems to be related with TSST-1 positive ST5 Geraldine clone (ST5/spat002/SCCmecI/agrII) frequently found in hospital and community environment in France.…”

Section: Discussionmentioning

confidence: 99%

“…However, the presence of this clone in Spain has been steadily declining, being replaced by international EMRSA-16 clone (CC30/ST36/SCCmecII), very common in the UK. 4,6 Currently, this clone has an irregular representation in different institutions while CC5/ST125/SCCmecIV has become more prevalent. 16 High percentage of our isolates belonged to this CC5/ST125/SCCmecIV.…”

Section: Discussionmentioning

confidence: 99%

“…Pandemic spread of different hospital-associated MRSA (HA-MRSA) clones, as ST239/SCCmecIII-IV, EMRSA-16 or ST36/SCCmecII (CC30) and ST125/SCCmecIV (CC5) and communityassociated MRSA (CA-MRSA) clones, as ST1/SCCmecIV (CC1), USA 300 or ST8/SCCmecIV (CC8), ST30/SCCmecIV (CC30) has been observed. [4][5][6] To date, livestock-associated MRSA (LA-MRSA) clones has not been frequently identified in hospitals or nursing homes in Europe, although the spread seems to be dependent on the region and the intensity of pig farming. 7 In Spain in early twenties, the dominant clone was Iberian clone (ST247/SCCmecI) but over the years this clone has reduced its presence.…”

Section: Q2mentioning

confidence: 99%

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Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

González-Domínguez

Seral

Potel

et al. 2015

Enfermedades Infecciosas y Microbiología Clínica

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Please cite this article in press as: González-Domínguez M, et al. b s t r a c tIntroduction: MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment. Materials and methods: A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them.Results: Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide-lincosamide-streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6 )-Ie-aph(2 )-Ia + ant (4 ) El CC5 fue el más prevalente (88,1%) seguido de CC8 (5,9%), CC22 (2,5%), CC398 (2,5%) y CC1 (0,8%). Conclusión: La línea clonal CC5/ST125/t067 fue la más habitual. Esta línea se relacionó con resistencia a aminoglucósidos, y, en menor medida, con macrólidos. La presencia de clones internacionales como EMRSA-15 (CC22/ST22), clones europeos como CC5/ST228, clones comunitarios relacionados con CC1 o CC8 y clones asociados al ganado, como el CC398, se observaron en un bajo porcentaje. ARTICLE IN PRESSG Model EIMC 1282 1-7 2 M. González-Domínguez et al. / Enferm Infecc Microbiol Clin. 2015;xxx(xx):xxx-xxx

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Q2mentioning

confidence: 99%

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Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

González-Domínguez

Seral

Potel

et al. 2015

Enfermedades Infecciosas y Microbiología Clínica

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“…In contrast to phage type 80/ 81 and SWP, the EMRSA-16 (ST36) clone appears to be restricted to the hospital setting and has reduced virulence due in part to low levels of expression of cytolytic toxins (12). Along with the EMRSA-15 (ST22) clone, EMRSA-16 has been endemic in UK hospitals for over 20 y and has also been reported less commonly in other European countries, Southeast Asia, South Africa, Australia, and North America (3,(13)(14)(15)(16)(17). The high rate of MRSA infections and the rapid spread of HA-MRSA between UK hospitals led the UK government to introduce stringent infection control legislation from 2003, resulting in a decrease in rates of nosocomial MRSA infection (18,19).…”

mentioning

confidence: 99%

Molecular tracing of the emergence, adaptation, and transmission of hospital-associated methicillin-resistant Staphylococcus aureus

McAdam

Templeton

Edwards

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

173

139

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Hospital-associated infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a global health burden dominated by a small number of bacterial clones. The pandemic EMRSA-16 clone (ST36-II) has been widespread in UK hospitals for 20 y, but its evolutionary origin and the molecular basis for its hospital association are unclear. We carried out a Bayesian phylogenetic reconstruction on the basis of the genome sequences of 87 S. aureus isolates including 60 EMRSA-16 and 27 additional clonal complex 30 (CC30) isolates, collected from patients in three continents over a 53-y period. The three major pandemic clones to originate from the CC30 lineage, including phage type 80/81, Southwest Pacific, and EMRSA-16, shared a most recent common ancestor that existed over 100 y ago, whereas the hospital-associated EMRSA-16 clone is estimated to have emerged about 35 y ago. Our CC30 genome-wide analysis revealed striking molecular correlates of hospital-or community-associated pandemics represented by mobile genetic elements and nonsynonymous mutations affecting antibiotic resistance and virulence. Importantly, phylogeographic analysis indicates that EMRSA-16 spread within the United Kingdom by transmission from hospitals in large population centers in London and Glasgow to regional health-care settings, implicating patient referrals as an important cause of nationwide transmission. Taken together, the high-resolution phylogenomic approach used resulted in a unique understanding of the emergence and transmission of a major MRSA clone and provided molecular correlates of its hospital adaptation. Similar approaches for hospitalassociated clones of other bacterial pathogens may inform appropriate measures for controlling their intra-and interhospital spread.nosocomial | epidemiology

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“…2,3,9,10,[14][15][16] Co ok son ve ark. 11 Av ru pa ül ke sin de ki hasta ne ler den 1981-1998 yıl la rı ara sın da izo le edi len 98 MRSA ile yap tık la rı ça lış ma so nu cun da PFGE yön te mi ile üç ve ya da ha az bant fark lı lı ğı gös te ren 34 tip tes pit et miş ve en sık gö rü len Tip 3'ü oluş-tu ran 24 izo la tın ye di fark lı ül ke den el de edil di ği -ni bil dir miş ler dir.…”

Section: Tar Tiş Maunclassified

Genotyping of Methicillin Resistant Staphylococcus aureus Strains Causing Nosocomial Infections by Pulsed Field Gel Electrophoresis in Adana Çukurova University Hospital

Akcimen¹,

Nağıyev²,

Biçer³

et al. 2010

Turkiye Klinikleri J Med Sci

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Tracking Methicillin-Resistant Staphylococcus aureus Clones during a 5-Year Period (1998 to 2002) in a Spanish Hospital

Cited by 91 publications

References 49 publications

Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

Molecular tracing of the emergence, adaptation, and transmission of hospital-associated methicillin-resistant Staphylococcus aureus

Genotyping of Methicillin Resistant Staphylococcus aureus Strains Causing Nosocomial Infections by Pulsed Field Gel Electrophoresis in Adana Çukurova University Hospital

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