Tracking of single molecules as a powerful method to characterize diffusivity of organic species in mesoporous materials

Hellriegel, Christian; Kirstein, Johanna; Bräuchle, Christoph

doi:10.1088/1367-2630/7/1/023

Cited by 76 publications

(103 citation statements)

References 20 publications

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“…The lateral mobility of Cav1 vesicles near the basal PM of adherent cells was monitored by acquiring TIRFm image sequences and subsequent software-based vesicle tracking (Hellriegel et al, 2005). In each image, Cav1-GFP vesicles appeared as well-separated diffraction-limited spots of various intensities that were separated from each other by a few microns.…”

Section: High Spatiotemporal Resolution Particle Tracking For Cav1-gfpmentioning

confidence: 99%

Phosphorylated filamin A regulates actin-linked caveolae dynamics

Muriel

Echarri

Hellriegel

et al. 2011

Journal of Cell Science

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SummaryCaveolae are relatively stable membrane invaginations that compartmentalize signaling, regulate lipid metabolism and mediate viral entry. Caveolae are closely associated with actin fibers and internalize in response to diverse stimuli. Loss of cell adhesion is known to induce rapid and robust caveolae internalization and trafficking toward a Rab11-positive recycling endosome; however, pathways governing this process are poorly understood. Here, we report that filamin A is required to maintain the F-actin-dependent linear distribution of caveolin-1. High spatiotemporal resolution particle tracking of caveolin-1-GFP vesicles by total internal reflection fluorescence (TIRF) microscopy revealed that FLNa is required for the F-actin-dependent arrest of caveolin-1 vesicles in a confined area and their stable anchorage to the plasma membrane. The linear distribution and anchorage of caveolin-1 vesicles are both required for proper caveolin-1 inwards trafficking. De-adhesion-triggered caveolae inward trafficking towards a recycling endosome is impaired in FLNa-depleted HeLa and FLNa-deficient M2-melanoma cells. Inwards trafficking of caveolin-1 requires both the ability of FLNa to bind actin and cycling PKC-dependent phosphorylation of FLNa on Ser2152 after cell detachment.

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Section: High Spatiotemporal Resolution Particle Tracking For Cav1-gfpmentioning

confidence: 99%

Phosphorylated filamin A regulates actin-linked caveolae dynamics

Muriel

Echarri

Hellriegel

et al. 2011

Journal of Cell Science

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“…In contrast to those studies, we investigated the diffusional processes of various fluorescent dye molecules in the nanoporous network of sol-gel glasses [70] and in mesoporous materials [71] with the direct method of single molecule tracking. Furthermore we showed diffusional and orientational dynamics of various single dye molecules in nanostructured host-guest materials [72] and the switchable mobility of single TDI molecules in networks of long unidimensional nanochannels [73].…”

Section: Diffusion In Thin Silica Filmsmentioning

confidence: 99%

Review. Fluorescence Microscopy Studies of Porous Silica Materials

Rühle

Davies

Bein

et al. 2013

Zeitschrift Für Naturforschung B

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In this article, we discuss how fluorescence microscopy techniques are used to investigate important characteristics of porous silica materials. We start with a discussion of the synthesis, formation mechanism and functionalization of these materials. We then give an introduction to single molecule microscopy and show how this technique can be used to gain deeper insights into some defining properties of porous silica, such as pore structure, host-guest interactions and diffusion dynamics. We also provide examples from the literature demonstrating how fluorescence microscopy is used for elucidating important aspects of porous silica materials and heterogeneous catalysis, e. g. diffusion properties, reactivity, morphology, intergrowth, accessibility, and catalyst deactivation. Finally, a short outlook on the scope of porous silica hosts in drug delivery applications is given.

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“…This distribution can be visualized in two ways: either in the form of a histogram or through cumulative probabilities. 59,60 The analysis of probability distributions (instead of histograms) allows for a more precise analysis and avoids any loss of information due to binning of the histogram. Hence, the following analysis was done by plotting the inverse of the cumulative probability C(R 2 ,t) of the squared displacements r 2 for different time lags t. The data was fitted with multiexponential decay functions: Regular diffusion should result in a monoexponential decay (n = 1), giving a characteristic value for the MSD hr 2 i (t)i for each time lag t. Fig.…”

Section: Downloaded By Ludwig Maximiliansmentioning

confidence: 99%

The complexity of mesoporous silica nanomaterials unravelled by single molecule microscopy

Lebold

Michaelis

Bräuchle

2011

Phys. Chem. Chem. Phys.

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Mesoporous silica nanomaterials are a novel class of materials that offer a highly complex porous network with nanometre-sized channels into which a wide amount of differently sized guests can be incorporated. This makes them an ideal host for various applications for example in catalysis, chromatography and nanomedicine. For these applications, analyzing the host properties and understanding the complicated host-guest interactions is of pivotal importance. In this perspective we review some of our recent work that demonstrates that single molecule microscopy techniques can be utilized to characterize the porous silica host with unprecedented detail. Furthermore, the single molecule studies reveal sample heterogeneities and are a highly efficient tool to gain direct mechanistic insights into the host-guest interactions. Single molecule microscopy thus contributes to a thorough understanding of these nanomaterials enabling the development of novel tailor-made materials and hence optimizing their applicability significantly.

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Tracking of single molecules as a powerful method to characterize diffusivity of organic species in mesoporous materials

Cited by 76 publications

References 20 publications

Phosphorylated filamin A regulates actin-linked caveolae dynamics

Phosphorylated filamin A regulates actin-linked caveolae dynamics

Review. Fluorescence Microscopy Studies of Porous Silica Materials

The complexity of mesoporous silica nanomaterials unravelled by single molecule microscopy

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