Tracking the prokaryotic diversity in acid mine drainage-contaminated environments: A review of molecular methods

Mohapatra, Bidyut R.; Gould, W. Douglas; Dinardo, O.; Koren, David W.

doi:10.1016/j.mineng.2011.03.012

Cited by 35 publications

(18 citation statements)

References 97 publications

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“…The present study compares two geographically isolated mine waters using high-throughput sequencing of the 16S rRNA gene using a 500-bp amplicon generated from the universal bacterial primers 27 F and 518R (Lane 1991). Assessment of the microbial communities in mine waters will not only provide information about their ecophysiology and the ecophysiological adaptations required to cope with extreme environments; knowledge of these complex communities will also assist in developing and providing sustainable and reliable remediation solutions (Mohapatra et al 2011). The objectives of this research were to classify the bacteria present in the mine waters from South Africa and to compare the bacterial communities in the samples across geographic locations.…”

Section: Introductionmentioning

confidence: 99%

Profile of bacterial communities in South African mine-water samples using Illumina next-generation sequencing platform

Keshri

Mankazana

Momba

2014

Appl Microbiol Biotechnol

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Mine water is an example of an extreme environment that contains a large number of diverse and specific bacteria. It is imperative to gain an understanding of these bacterial communities in order to develop effective strategies for the bioremediation of polluted aquatic systems. In this study, the high-throughput sequencing approach was used to characterize the bacterial communities in two different mine waters of South Africa: vanadium and gold mine water. Over 2629 operational taxonomic units (OTUs) were recovered from 15,802 reads of the 16S ribosomal RNA (rRNA) gene. They represented 8 phyla, 43 orders, 84 families and 105 genera. Proteobacteria and unclassified bacterial sequences were the most dominant. Apart from these, Firmicutes, Bacteroidetes, Actinobacteria, Candidate phylum OD1, Cyanobacteria, Verrucomicrobia and Deinococcus-Thermus were the recovered phyla, although their relative abundance differed between both the mine-water samples. Yet, diversity indices suggested that the bacterial communities inhabiting the vanadium mine water were more diverse than those in gold mine water. Interestingly, substantial percentages of the reads from either sample (58 % in vanadium and 17 % in gold mine water) could not be assigned to any phylum and remained unclassified, suggesting hitherto unidentified populations, and vast untapped microbial diversity. Overall, the results of this study exhibited bacterial community structures with high diversity in mine water, which can be explored further for their role in bioremediation and environmental management.

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Section: Introductionmentioning

confidence: 99%

Profile of bacterial communities in South African mine-water samples using Illumina next-generation sequencing platform

Keshri

Mankazana

Momba

2014

Appl Microbiol Biotechnol

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“…Some mesophilic iron(II)-oxidizing archaeons belonging to the Thermoplasmales have also been isolated and described: Ferroplasma acidiphilium and F. acidarmanus. In fact, a consortium of different microorganisms is responsible for the oxidative reactions, resulting in the extraction of dissolved metal values from ores (Schippers, 2007;Watling et al, 2010;Mohapatra et al, 2011 and references therein).…”

Section: Biomining Extremophiles and Their Industrial Applicationsmentioning

confidence: 99%

The Use of Extremophilic Microorganisms in the Industrial Recovery of Metals

Jerez

2012

Extremophiles

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“…; Mohapatra et al . ). A passive treatment technology often operates without mechanized assistance, and incorporates the physical/chemical, or biological processes that often occur naturally in the environment (Jennings et al .…”

Section: Introductionmentioning

confidence: 97%

“…; Mohapatra et al . ). Most of passive treatment technologies are based on phytoremediation principles associated with wetland ecosystems.…”

Section: Introductionmentioning

confidence: 97%

“…Active AMD treatment systems consist of highly engineered water treatment facilities such as chemical dosing, and nanofiltration (Jennings et al 2008;Mohapatra et al 2011). A passive treatment technology often operates without mechanized assistance, and incorporates the physical/chemical, or biological processes that often occur naturally in the environment (Jennings et al 2008;Mohapatra et al 2011). Most of passive treatment technologies are based on phytoremediation principles associated with wetland ecosystems.…”

Section: Introductionmentioning

confidence: 99%

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Potential of citric acid to alter pH and metal uptake in reeds in acid mine drainage solutions

Guo

Cutright

2017

Water & Environment J

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Phragmites australis (common reed) is often used to treat acid mine drainage (AMD) sites contaminated with high concentrations of heavy metals and low pH. This study investigated the effects of different levels (low 0.34 g/L, middle 17.86 g/L and high 33.62 g/L) of the biodegradable chelator citric acid (CA) on pH change, metal (i.e. Fe, Al and Mn) accumulation and translocation in reeds cultured in aqueous AMD contaminated media for 4 and 12 weeks. The results indicated that CA inhibited further deceases in AMD pH as well as increased Fe and Mn accumulation in both belowground and above ground tissues of reeds. As expected, increasing CA dosage and treatment time increased metal accumulation in plants. By 12 weeks, 0.18 ± 0.01 mg Mn/g, 56.78 ± 7.63 mg Fe/g and 0.60 ± 0.01 mg Al/g were accumulated in roots; while 0.06 ± 0.01 mg Mn/g; 6.38 ± 0.06 mg Fe/g; and 0.19 ± 0.01 mg Al/g were sequestered in stems of reeds treated with 33.62 g/L CA. Further research is needed to study the application of CA to enhance the phytoremediation efficiency of AMD contaminated field.

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Tracking the prokaryotic diversity in acid mine drainage-contaminated environments: A review of molecular methods

Cited by 35 publications

References 97 publications

Profile of bacterial communities in South African mine-water samples using Illumina next-generation sequencing platform

Profile of bacterial communities in South African mine-water samples using Illumina next-generation sequencing platform

The Use of Extremophilic Microorganisms in the Industrial Recovery of Metals

Potential of citric acid to alter pH and metal uptake in reeds in acid mine drainage solutions

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