Tracking viral movement in plants by means of chlorophyll fluorescence imaging

Pineda, Mónica; Olejníčková, Julie; Cséfalvay, Ladislav; Barón, Matilde

doi:10.1016/j.jplph.2011.06.013

Cited by 19 publications

(27 citation statements)

References 30 publications

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“…The decrease in NPQ values in the front of virus invasion detected by P erez- Bueno et al (2006) might correlate with the early decrease of NPQ values in our experiments. The movement of PMMoV through the vascular system and its subsequent spread into the leaves was also detectable by chlorophyll a fluorescence imaging (Pineda et al, 2011). The altered photochemical energy conversion by both ObPV and PMMoV would suggest that the xanthophyll cycle is involved in antiviral response of pepper.…”

Section: Discussionmentioning

confidence: 97%

Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

Rys

Juhász

Surówka

et al. 2014

Plant Physiology and Biochemistry

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imaging technique. PMMoV inoculation also led to a significant rise of ion leakage and heat emission, to the up-regulation of the pepper CCD gene as well as to decreased PSII efficiency, but these responses were much weaker than in the case of ObPV inoculation. Chlorophyll b and total carotenoid contents as measured by spectrophotometric methods were not significantly influenced by any virus inoculations when these pigment contents were calculated on leaf surface basis. On the other hand, near-infrared FTRaman spectroscopy showed an increase of carotenoid content in ObPV-inoculated leaves suggesting that the two techniques detect different sets of compounds.

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Section: Discussionmentioning

confidence: 97%

Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: Chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy

Rys

Juhász

Surówka

et al. 2014

Plant Physiology and Biochemistry

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“…Non-invasive techniques to image the patterns of multispectral fluorescence or leaf temperature across infected leaves have greatly increased our understanding of plant responses to biotic stress [1,2]. Visualization of the light signals emitted by plants can track the spreading of a pathogen through its host [3][4][5]. Furthermore, these techniques could be very useful for presymptomatic stress detection, depending on the extent in the changes in plants metabolism in response to pathogens.…”

Section: Imaging Techniques For Biotic Stress Detectionmentioning

confidence: 99%

“…Thus, Chl-FI adds spatial resolution to the information obtained by conventional fluorometers. Changes in Chl-FI parameters could be related to physiological alterations in plants infected by viruses [4,5,[22][23][24][25][26][27][28], bacteria [29][30][31][32][33][34] and fungi [35][36][37][38].…”

Section: Imaging Kinetics Of Chlorophyll Fluorescencementioning

confidence: 99%

“…Chl-FI measurements have been carried out in both inoculated and systemically-infected leaves during viral challenge, showing photosynthesis impairment in symptomatic and asymptomatic areas during pathogenesis [4,5,24,25,27]. In some cases, the rate of photosynthetic inhibition is associated to the severity of the symptoms; however, changes in some fluorescence parameters could precede the development of symptoms, allowing a presymptomatic detection of the disease [40].…”

Section: Chlorophyll Fluorescence Imaging To Monitor Plants Infected mentioning

confidence: 99%

“…In some cases, the rate of photosynthetic inhibition is associated to the severity of the symptoms; however, changes in some fluorescence parameters could precede the development of symptoms, allowing a presymptomatic detection of the disease [40]. Correlation between the pattern of Chl-FI quenching and virus distribution in leaves was found during the infection of pepper mild mottle virus (PMMoV) in Nicotiana benthamiana plants [4,5] as well as in Abutilon mosaic virusinfected Abutilon striatum leaves [24,25]. Asymptomatic leaves of N. benthamiana infected with PMMoV, showed low NPQ values around the invasion front, suggesting enhancement of the photosynthetic electron transport rate.…”

Section: Chlorophyll Fluorescence Imaging To Monitor Plants Infected mentioning

confidence: 99%

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Picturing pathogen infection in plants

Barón

Pineda

Pérez-Bueno

2016

Zeitschrift Für Naturforschung C

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Several imaging techniques have provided valuable tools to evaluate the impact of biotic stress on host plants. The use of these techniques enables the study of plant-pathogen interactions by analysing the spatial and temporal heterogeneity of foliar metabolism during pathogenesis. In this work we review the use of imaging techniques based on chlorophyll fluorescence, multicolour fluorescence and thermography for the study of virus, bacteria and fungi-infected plants. These studies have revealed the impact of pathogen challenge on photosynthetic performance, secondary metabolism, as well as leaf transpiration as a promising tool for field and greenhouse management of diseases. Images of standard chlorophyll fluorescence (Chl-F) parameters obtained during Chl-F induction kinetics related to photochemical processes and those involved in energy dissipation, could be good stress indicators to monitor pathogenesis. Changes on UV-induced blue (F440) and green fluorescence (F520) measured by multicolour fluorescence imaging in pathogen-challenged plants seem to be related with the up-regulation of the plant secondary metabolism and with an increase in phenolic compounds involved in plant defence, such as scopoletin, chlorogenic or ferulic acids. Thermal imaging visualizes the leaf transpiration map during pathogenesis and emphasizes the key role of stomata on innate plant immunity. Using several imaging techniques in parallel could allow obtaining disease signatures for a specific pathogen. These techniques have also turned out to be very useful for presymptomatic pathogen detection, and powerful non-destructive tools for precision agriculture. Their applicability at lab-scale, in the field by remote sensing, and in high-throughput plant phenotyping, makes them particularly useful. Thermal sensors are widely used in crop fields to detect early changes in leaf transpiration induced by both air-borne and soil-borne pathogens. The limitations of measuring photosynthesis by Chl-F at the canopy level are being solved, while the use of multispectral fluorescence imaging is very challenging due to the type of light excitation that is used.Keywords: biotic stress; chlorophyll fluorescence imaging; multicolor fluorescence imaging; plant pathogens; thermography. Dedication: This work is dedicated to the memory of Professor Peter Böger. He will always bring to my heart (M. B.) the best memories of my postdoc at Lake Constance.

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