Traction proteins in the extracellular matrix of Dictyostelium discoideum slugs

Cellulose-binding modules (CBMs) of two extracellular matrix proteins, St15 and ShD, from the slime mold Dictyostelium discoideum were expressed in Escherichia coli. The expressed proteins were purified to . 98% purity by extracting inclusion bodies at pH 11.5 and refolding proteins at pH 7.5. The two refolded CBMs bound tightly to amorphous phosphoric acid swollen cellulose (PASC), but had a low affinity toward xylan. Neither protein exhibited cellulase activity. St15, the stalk-specific protein, had fourfold higher binding affinity toward microcrystalline cellulose (Avicel) than the sheath-specific ShD CBM. St15 is unusual in that it consists of a solitary CBM homologous to family IIa CBMs. Sequence analysis of ShD reveals three putative domains containing: (a) a C-terminal CBM homologous to family IIb CBMs; (b) a Pro/ Thr-rich linker domain; and (c) a N-terminal Cys-rich domain. The biological functions and potential role of St15 and ShD in building extracellular matrices during D. discoideum development are discussed.

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“…[20] from D. discoideum; D. discoideum CBM (clone , accession no. 11). Key functional tryptophan residues are shaded in black.…”

Section: Discussionmentioning

confidence: 99%

Cellulose‐binding modules from extracellular matrix proteins of Dictyostelium discoideum stalk and sheath

Wang

Slade

Gooley³

et al. 2001

European Journal of Biochemistry

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“…These glycoproteins are essentially undetectable in slug cells (Grant & Williams, 1983;Alexander et al, 1988). Evidence is mounting that glycoproteins that carry the modB-dependent glycosylation have a role in slug traction (Vardy et al, 1986;Breen et ul., 1987;Breen & Williams, 1988). The mod-352/mod-353-dependent glycosylation occurs on a different group of extracellular matrix proteins that may have a role in lubrication of slug movement, as determined by immunofluorescence microscopy of intact trails (Breen & Williams, 1988).…”

Section: Discussionmentioning

confidence: 99%

Immunodominant carbohydrate determinants in the multicellular stages of Dictyostelium discoideum

Champion

Callaghan

et al. 1991

Journal of General Microbiology

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Two families of glycoprotein are defined in DictyosteZium discoideum by the presence of different glycoconjugates, both of which are highly immunogenic in mice. The previously described monoclonal antibodies MUD50 and MUD62 recognize the glycoconjugates and identify the respective glycoprotein families. Both types of glycosylation occur on vegetative and developmentally regulated glycoproteins. The immunodominant components of both families are reportedly 0-linked sugars, but Western blots do not identify any glycoprotein that has both 0-glycans, suggesting that there are two independently processed types of 0-linked glycosylation in D. discoideum.The synthesis of the two 0-glycan families is affected by glycosylation-defective mutations. Strains with a mutation at the modB locus lack one of these glycosylation types (that recognized by MUDSO) and this mutation alters the size of two minor glycoproteins in the second family. Two new mutants, HU2470 (mod-352) and HU2471 (mod-353), lack the epitope recognized by MUD62. The two mutations map to different chromosomes. The mod-353 mutation also affects the size of PsA, a cell surface glycoprotein carrying the modB-dependent 0-glycan.

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“…In addition they are kept together by a sheath, which like an extracellular matrix surrounds the body, providing it with a smooth, poorly permeable surface (16). During the growth phase cells exist as individual amoebae.…”

Section: Overview Of Dictyostelium Developmentmentioning

confidence: 99%

CYCLIC AMP AND OTHER SIGNALS CONTROLLING CELL DEVELOPMENT AND DIFFERENTIATION IN DICTYOSTELIUM

Gerisch¹

1987

Annu. Rev. Biochem.

234

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Traction proteins in the extracellular matrix of Dictyostelium discoideum slugs

Cited by 27 publications

References 11 publications

Cellulose‐binding modules from extracellular matrix proteins of Dictyostelium discoideum stalk and sheath

Cellulose‐binding modules from extracellular matrix proteins of Dictyostelium discoideum stalk and sheath

Immunodominant carbohydrate determinants in the multicellular stages of Dictyostelium discoideum

CYCLIC AMP AND OTHER SIGNALS CONTROLLING CELL DEVELOPMENT AND DIFFERENTIATION IN DICTYOSTELIUM

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