Traditionally brewed rice beverage alters gut microbiota and metabolites in mice

Abstract

The study is an attempt to validate the effects of fermented rice beverage on gut microbiota and health, using mouse model. Rice beverage, soluble and insoluble fractions were fed to the animals for 30 days. Gut bacteria and metabolites were studied on 0th and 30th days of treatments. Next generation of sequencing of fecal metagenomic 16S rDNA indicated changes in abundance of 17 bacteria in the rice beverage treatment followed by 11 and 7 respectively in the soluble and insoluble fractions. Fecal metabolites of treatments as determined by GC-MS analyses, clustered differently based on Partial least square discriminant analysis. There were 253 negative and 160 positive correlations between relative abundance of fecal metabolites with bacteria. Histological analysis showed that microbial changes and higher plasma endotoxin levels in insoluble fraction treated group were accompanied by increased lymphocytes infiltration in lamina propia and cytoclasis of goblet cells as demonstrated by intestinal histology.