TRAIL is involved in CpG ODN-mediated anti-apoptotic signals

Lim, Eunjung; Park, Dae-Weon; Jeong, Tae-Whal; Chin, Byung-Rho; Bae, Yoe‐Sik; Baek, Suk‐Hwan

doi:10.3892/or.2011.1579

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…However, it is supported by data showing that short-term (48 h) exposure of macrophages to LPS or CpG prolongs cell survival (21,23,24). Interestingly, we show Poly I:C had no effect on the viability of these cells.…”

Section: Discussionsupporting

confidence: 74%

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TLR7 and TLR9 ligands regulate antigen presentation by macrophages

Celhar

Pereira-Lopes

Thornhill

et al. 2015

International Immunology

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The toll-like receptors (TLRs) are important innate receptors recognizing potentially pathogenic material. However, they also play a significant role in the development of Alzheimer's disease, cancer, autoimmunity and the susceptibility to viral infections. Macrophages are essential for an effective immune response to foreign material and the resolution of inflammation. In these studies, we examined the impact of different TLR ligands on macrophage cell function. We demonstrate that stimulation of all TLRs tested increases the phagocytosis of apoptotic cells by macrophages. TLR7 and TLR9 ligation decreased the levels of the surface co-expression molecules CD86 and MHCII, which was associated with a concomitant reduction in antigen presentation and proliferation of T cells. This down-regulation in macrophage function was not due to an increase in cell death. In fact, exposure to TLR7 or TLR9 ligands promoted cell viability for up to 9 days, in contrast to TLR3 or TLR4. Additionally, macrophages exposed to TLR7/TLR9 ligands had a significantly lower ratio of Il-12/Il-10 mRNA expression compared with those treated with the TLR4 ligand, LPS. Taken together, these data demonstrate that TLR7/TLR9 ligands push the macrophage into a phagocytic long-lived cell, with a decreased capacity of antigen presentation and reminiscent of the M2 polarized state.

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Section: Discussionsupporting

confidence: 74%

“…4B-D). These results are supported by previous studies indicating that LPS or CpG can induce short-term (48 h) survival of macrophages (21,23,24).…”

Section: Tlr7 and Tlr9 Ligands Induce Long-term Survival Of Macrophagessupporting

confidence: 81%

TLR7 and TLR9 ligands regulate antigen presentation by macrophages

Celhar

Pereira-Lopes

Thornhill

et al. 2015

International Immunology

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“…In addition, CpG‐ODN markedly increased the levels of Bcl2 both in sham control and I/R mice. The data indicate that the anti‐apoptotic effect of CpG‐ODN may be one of the mechanisms by which CpG‐ODN attenuated cerebral I/R injury.…”

Section: Discussionmentioning

confidence: 90%

The TLR9 Ligand, CpG‐ODN, Induces Protection against Cerebral Ischemia/Reperfusion Injury via Activation of PI3K/Akt Signaling

Chen

Wang

et al. 2014

JAHA

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BackgroundToll‐like receptors (TLRs) have been shown to be involved in cerebral ischemia/reperfusion (I/R) injury. TLR9 is located in intracellular compartments and recognizes CpG‐DNA. This study examined the effect of CpG‐ODN on cerebral I/R injury.Methods and ResultsC57BL/6 mice were treated with CpG‐ODN by i.p. injection 1 hour before the mice were subjected to cerebral ischemia (60 minutes) followed by reperfusion (24 hours). Scrambled‐ODN served as control‐ODN. Untreated mice, subjected to cerebral I/R, served as I/R control. The effect of inhibitory CpG‐ODN (iCpG‐ODN) on cerebral I/R injury was also examined. In addition, we examined the therapeutic effect of CpG‐ODN on cerebral I/R injury by administration of CpG‐ODN 15 minutes after cerebral ischemia. CpG‐ODN administration significantly decreased cerebral I/R‐induced infarct volume by 69.7% (6.4±1.80% vs 21.0±2.85%, P<0.05), improved neurological scores, and increased survival rate, when compared with the untreated I/R group. Therapeutic administration of CpG‐ODN also significantly reduced infarct volume by 44.7% (12.6±2.03% vs 22.8±2.54%, P<0.05) compared with untreated I/R mice. Neither control‐ODN, nor iCpG‐ODN altered I/R‐induced cerebral injury or neurological deficits. Nissl staining showed that CpG‐ODN treatment preserved neuronal morphology in the ischemic hippocampus. Immunoblot showed that CpG‐ODN administration increased Bcl‐2 levels by 41% and attenuated I/R‐increased levels of Bax and caspase‐3 activity in ischemic brain tissues. Importantly, CpG‐ODN treatment induced Akt and GSK‐3β phosphorylation in brain tissue and cultured microglial cells. PI3K inhibition with LY294002 abolished CpG‐ODN‐induced protection.ConclusionCpG‐ODN significantly reduces cerebral I/R injury via a PI3K/Akt‐dependent mechanism. Our data also indicate that CpG‐ODN may be useful in the therapy of cerebral I/R injury.

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Sonneralignan A, a new lignan from the fruit of mangrove Sonneratia apetala

Li,

Xia,

Bai

et al. 2023

Natural Product Research

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A new lignin, sonneralignan A (1), along with two known lignan compounds, (+)-lariciresinol-9-O-β-D-glucopyranoside (2) and (-)isolariciresinol-9-O-β-D-glucopyranoside (3) were isolated from the n-butanol extract of the mangrove Sonneratia apetala fruit. The structures of the compounds were elucidated on the basis of extensive spectral analysis. The evaluation of activity showed that compound 1 exhibited significant anti-aging activity, which extended the mean lifespan of Caenorhabditis elegans by up to 19.13% (P < 0.05) and 55.29% (P < 0.01) under normal and heat stress cultivation conditions, respectively. Molecular docking studies suggested that compound 1 was bound to the DNA binding domain of DAF-16 and promoted the conformation of DAF-16, thus strengthening the interaction between the DAF-16 and related DNA. TRP-252, SER-250 and SER-249 of the binding region might be the key amino residues during the interaction.

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TRAIL is involved in CpG ODN-mediated anti-apoptotic signals

Cited by 5 publications

References 29 publications

TLR7 and TLR9 ligands regulate antigen presentation by macrophages

TLR7 and TLR9 ligands regulate antigen presentation by macrophages

The TLR9 Ligand, CpG‐ODN, Induces Protection against Cerebral Ischemia/Reperfusion Injury via Activation of PI3K/Akt Signaling

Sonneralignan A, a new lignan from the fruit of mangrove Sonneratia apetala

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