Trans -10, cis -12 conjugated linoleic acid (CLA) interferes with lipid droplet accumulation during 3T3-L1 preadipocyte differentiation

Yeganeh, Azadeh; Taylor, Carla G.; Tworek, Leslee; Poole, Jenna; Zahradka, Peter

doi:10.1016/j.biocel.2016.04.013

Cited by 10 publications

(12 citation statements)

References 48 publications

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“…The decreased glycerol release in our experiments under chronic exposure to CLA was unexpected, but would concur with reduced glycerol production (Yeganeh et al, 2016) under a long-term (8 days) treatment with t10-c12-CLA in differentiating 3T3-L1 cells. On the contrary, 3T3-L1 adipocytes treated with trans-10, cis-12 CLA for 6 days increased basal but not isoproterenol-stimulated lipolysis in relation to the control (Evans et al, 2002).…”

Section: Lipolysissupporting

confidence: 76%

“…On the contrary, 3T3-L1 adipocytes treated with trans-10, cis-12 CLA for 6 days increased basal but not isoproterenol-stimulated lipolysis in relation to the control (Evans et al, 2002). Other authors have reported no changes in lipolysis elicited by trans-10, cis-12 CLA in long-term (48 h) adipose tissue explant cultures of pigs (José et al, 2008) and mature 3T3-L1 cells (Yeganeh et al, 2016). The contrasting responses found in the literature regarding CLA effects on lipolysis could be explained by the differences in lipid metabolism among species, time of culture and culture conditions.…”

Section: Lipolysismentioning

confidence: 88%

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Conjugated linoleic acid and betaine affect lipolysis in pig adipose tissue explants

Fernández‐Fígares

Lachica

M³

et al. 2019

Animal

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Consumers’ demand of leaner meat products is a challenge. Although betaine and conjugated linoleic acid (CLA) have the potential to decrease porcine adipose tissue, their mode of action is poorly understood. The aim of the study was to determine the lipolytic effect of betaine and CLA in the adipose tissue of Iberian pigs. Adipose tissue explants from five pigs (38 kg BW) were prepared from dorsal subcutaneous adipose tissue samples and cultivated for 2 h (acute experiments) or 72 h (chronic experiments). Treatments included 100 µM linoleic acid (control), 100 µM trans-10, cis-12 CLA, 100 µM linoleic acid + 1 mM betaine and 100 µM trans-10, cis-12 CLA + 1 mM betaine (CLABET). To examine the ability of betaine or CLA to inhibit insulin’s suppression of isoproterenol-stimulated lipolysis, test medium was amended with 1 µM isoproterenol ±10 nM insulin. Media glycerol was measured at the end of the incubations. Acute lipolysis (2 h) was increased by CLA and CLABET (85% to 121%; P < 0.05) under basal conditions. When lipolysis was stimulated with isoproterenol (1090%), acute exposure to betaine tended to increase (13%; P = 0.071), while CLA and CLABET increased (14% to 18%; P < 0.05) isoproterenol-stimulated lipolysis compared with control. When insulin was added to isoproterenol-stimulated explants, lipolytic rate was decreased by 50% (P < 0.001). However, supplementation of betaine to the insulin + isoproterenol-containing medium tended to increase (P = 0.07), while CLABET increased (45%; P < 0.05) lipolysis, partly counteracting insulin inhibition. When culture was extended for 72 h, CLA decreased lipolysis under basal conditions (18%; P < 0.05) with no effect of betaine and CLABET (P > 0.10). When lipolysis was stimulated by isoproterenol (125% increase in rate compared with basal), CLA and CLABET decreased glycerol release (27%; P < 0.001) compared with control (isoproterenol alone). When insulin was added to isoproterenol-stimulated explants, isoproterenol stimulation of lipolysis was completely blunted and neither betaine nor CLA altered the inhibitory effect of insulin on lipolysis. Isoproterenol, and especially isoproterenol + insulin, stimulated leptin secretion compared with basal conditions (68% and 464%, respectively; P < 0.001), with no effect of CLA or betaine (P > 0.10). CLA decreased leptin release (25%; P < 0.001) when insulin was present in the media, partially inhibiting insulin stimulation of leptin release. In conclusion, betaine and CLA produced a biphasic response regarding lipolysis so that glycerol release was increased in acute conditions, while CLA decreased glycerol release and betaine had no effect in chronic conditions. Furthermore, CLA and CLABET indirectly increased lipolysis by reducing insulin-mediated inhibition of lipolysis during acute conditions.

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Section: Lipolysissupporting

confidence: 76%

Section: Lipolysismentioning

confidence: 88%

Conjugated linoleic acid and betaine affect lipolysis in pig adipose tissue explants

Fernández‐Fígares

Lachica

M³

et al. 2019

Animal

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“…Administration of a mixture of CLA isomers (predominantly containing equal amounts of the c 9, t 11 and t 10, c 12 isomers) has been linked to a reduction in fat mass in a number of animal and human studies 7 – 9 . Studies administering the isomers in isolation suggest that the t 10, c 12 isomer is primarily responsible for reducing fat mass 10 , which may be mediated by reducing fat storage in mature adipocytes and therefore reducing adipocyte size 11 , 12 . In contrast, the c 9 ,t 11 isomer has been reported to promote adipogenesis through induction of peroxisome proliferator-activated receptor-γ (PPARγ) 13 .…”

Section: Introductionmentioning

confidence: 99%

Conjugated Linoleic Acid Supplementation Improves Maternal High Fat Diet-Induced Programming of Metabolic Dysfunction in Adult Male Rat Offspring

Segovia

Vickers

Gray

et al. 2017

Sci Rep

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The developmental origins of health and disease hypothesis proposes that an adverse early life environment, including in utero exposure to a maternal obesogenic environment, can lead to an increased long-term risk of obesity and related metabolic complications in offspring. We assessed whether maternal supplementation with conjugated linoleic acid (CLA) could prevent some of these adverse effects in offspring exposed to a maternal high fat diet. Sprague-Dawley dams consumed either a: control (CD), control with CLA (CLA), high fat (HF) or high fat with CLA (HFCLA) diet 10 days prior to mating and throughout pregnancy/lactation. Male offspring were weaned onto a standard chow diet. Body composition was quantified by DXA and oral glucose tolerance tests conducted on adult offspring. Gene/protein expression and histological analysis were conducted in adipose tissue. Offspring from HF dams had increased body weight, body fat deposition, impaired insulin sensitivity and adipocyte hypertrophy; all of which were rescued in HFCLA offspring. Molecular and histological analyses of the adipose tissue suggest that disturbances in adipogenesis may mediate the metabolic dysfunction observed in HF offspring. Therefore, CLA supplementation to a maternal obesogenic diet may be a promising strategy to prevent adverse programming outcomes.

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“…Unexpectedly, we obtained no differences in the lipid content of cells with CLA treatment, while other authors showed that 10 mg/L of CLA isomers, consisting principally of 41% c9, t11 and 44% t10, c12, inhibited proliferation of 3T3-L1 preadipocytes while promoting lipogenesis [ 55 ]. Other experiments have shown that the effects of CLA are truly influenced by the isomer used and the stage at which differentiation is applied [ 9 , 56 , 57 ]. Altogether, it appears that in cell culture experiments, the isomer t10, c12 has a more evident effect on inhibiting cell differentiation, while the isomer c9, t11 might increase lipid accumulation and promote preadipocyte differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Although adipocytes are the principal cells in WAT, it also includes a heterogeneous cell population called the stromal vascular fraction (SVF) [ 6 ]. Adipocytes have been extensively studied in vitro in the murine 3T3-L1 cell line, which is a good model to study the effects of particular compounds on adipocyte differentiation [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

A Mix of Natural Bioactive Compounds Reduces Fat Accumulation and Modulates Gene Expression in the Adipose Tissue of Obese Rats Fed a Cafeteria Diet

et al. 2020

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Scientists are focusing on bioactive ingredients to counteract obesity. We evaluated whether a mix containing grape seed proanthocyanidin extract (GSPE), anthocyanins, conjugated linoleic acid (CLA), and chicken feet hydrolysate (CFH) could reduce body fat mass and also determined which mechanisms in the white adipose tissue (WAT) and the brown adipose tissue (BAT) were affected by the treatment. The mix or vehicle (VH) were administered for three weeks to obese rats fed a cafeteria (CAF) diet. Biometric measures, indirect calorimetry, and gene expression in WAT and BAT were analyzed as was the histology of the inguinal WAT (IWAT). The individual compounds were also tested in the 3T3-L1 cell line. The mix treatment resulted in a significant 15% reduction in fat (25.01 ± 0.91 g) compared to VH treatment (21.19 ± 1.59 g), and the calorimetry results indicated a significant increase in energy expenditure and fat oxidation. We observed a significant downregulation of Fasn mRNA and an upregulation of Atgl and Hsl mRNA in adipose depots in the group treated with the mix. The IWAT showed a tendency of reduction in the number of adipocytes, although no differences in the total adipocyte area were found. GSPE and anthocyanins modulated the lipid content and downregulated the gene and protein levels of Fasn compared to the untreated group in 3T3-L1 cells. In conclusion, this mix is a promising treatment against obesity, reducing the WAT of obese rats fed a CAF diet, increasing energy expenditure and fat oxidation, and modifying the expression of genes involved in lipid metabolism of the adipose tissue.

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Trans -10, cis -12 conjugated linoleic acid (CLA) interferes with lipid droplet accumulation during 3T3-L1 preadipocyte differentiation

Cited by 10 publications

References 48 publications

Conjugated linoleic acid and betaine affect lipolysis in pig adipose tissue explants

Conjugated linoleic acid and betaine affect lipolysis in pig adipose tissue explants

Conjugated Linoleic Acid Supplementation Improves Maternal High Fat Diet-Induced Programming of Metabolic Dysfunction in Adult Male Rat Offspring

A Mix of Natural Bioactive Compounds Reduces Fat Accumulation and Modulates Gene Expression in the Adipose Tissue of Obese Rats Fed a Cafeteria Diet

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