Transcript profiling for regulation of sweet potato skin color in Sushu8 and its mutant Zhengshu20

Yang, Yufeng; Shi, Dianyi; Wang, Yannan; Zhang, Li; Chen, Xiangong; Yang, Xiaoping; Xiong, Haizheng; Bhattarai, Gehendra; Ravelombola, Waltram; Olaoye, Dotun; Yang, Gangyi; Shi, Ainong

doi:10.1016/j.plaphy.2019.12.035

Cited by 13 publications

(10 citation statements)

References 66 publications

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“…Sweet potato is planted widely in the world, and has hundreds of varieties or lines due to artificial selection, natural hybrids, and mutations [ 1 , 4 ]. The root tuber of sweet potato has different colored skin and flesh, but starch properties have no significant correlation with skin and flesh color, and are determined by the genotypes of varieties [ 2 , 10 , 11 ]. Starches from different sweet potato varieties have been studied for their structures, functional properties, and applications [ 10 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Sizes, Components, Crystalline Structure, and Thermal Properties of Starches from Sweet Potato Varieties Originating from Different Countries

Zhao

Shi

et al. 2022

Molecules

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Sweet potato is a root tuber crop and an important starch source. There are hundreds of sweet potato varieties planted widely in the world. Starches from varieties with different genotype types and originating from different countries have not been compared for their physicochemical properties. In the research, starches from 44 sweet potato varieties originating from 15 countries but planted in the same growing conditions were investigated for their physicochemical properties to reveal the similarities and differences in varieties. The results showed that the 44 starches had granule size (D[4,3]) from 8.01 to 15.30 μm. Starches had different iodine absorption properties with OD680 from 0.259 to 0.382 and OD620/550 from 1.142 to 1.237. The 44 starches had apparent amylose content from 19.2% to 29.2% and true amylose content from 14.2% to 20.2%. The starches exhibited A-, CA-, CC-, or CB-type X-ray diffraction patterns. The thermograms of 44 starches exhibited one-, two-, or three-peak curves, leading to a significantly different gelatinization temperature range from 13.1 to 29.2 °C. The significantly different starch properties divide the 44 sweet potato varieties into different groups due to their different genotype backgrounds. The research offers references for the utilization of sweet potato germplasm.

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Section: Introductionmentioning

confidence: 99%

Sizes, Components, Crystalline Structure, and Thermal Properties of Starches from Sweet Potato Varieties Originating from Different Countries

Zhao

Shi

et al. 2022

Molecules

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“…The RS color of the mutant changed from red to yellow, and the purple color of LVB faded. The variation of skin color was similar to that of two sweetpotato lines Sushu 8 (with red skin) and Zhengshu 20 (with yellow skin, a mutant of Sushu 8) …”

Section: Resultsmentioning

confidence: 99%

“…The variation of skin color was similar to that of two sweetpotato lines Sushu 8 (with red skin) and Zhengshu 20 (with yellow skin, a mutant of Sushu 8). 26 Total Content of Flavonoids and Anthocyanins. As mentioned above, the mutation affected the color of LVB and RS.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The mixture was then mixed with 4% NaOH, and the absorbance was measured at 320 nm. The anthocyanin contents in LVB and RS were determined following the colorimetric method reported by Tian et al 18 and Yang et al 26 The sample powder was blended with methanol containing 10% formic acid. With ultrasonic treatment, the mixture was centrifuged and the supernatant was measured at 520 nm.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Metabolomic and Transcriptomic Analyses of the Flavonoid Biosynthetic Pathway for the Accumulation of Anthocyanins and Other Flavonoids in Sweetpotato Root Skin and Leaf Vein Base

Zhao

Liu

et al. 2022

J. Agric. Food Chem.

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Sweetpotato [Ipomoea batatas (L.) Lam.] is a major tuberous root crop that is rich in flavonoids. Here, we discovered a spontaneous mutation in the color of the leaf vein base (LVB) and root skin (RS) in the Zheshu 81 cultivar. The flavonoid and anthocyanin metabolites and molecular mechanism were analyzed using metabolome and transcriptome data. Compared to the wild type, 13 differentially accumulated metabolites (DAMs) in the LVB and 59 DAMs in the RS were all significantly downregulated. Moreover, all the anthocyanin metabolites decreased significantly. The differentially expressed genes (DEGs) encoding the key enzymes in the later enzymatic reaction of anthocyanin and flavonoid were significantly downregulated in the mutant. The expression trends of the transcription factor MYB were evidently related to the anthocyanin content. These results offer insights into the coloration in the LVB and RS and a theoretical basis for determining the regulation of flavonoid and anthocyanin synthesis in sweetpotato.

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“…To investigate the key anthocyanin-related IbR2R3-MYBs in sweet potatoes, multiple public RNA-seq datasets representing different color variants in sweet potato leaf and tuberous root flesh and skin were analyzed [8,43,56,57]. These datasets focused on searching all potential candidate genes of anthocyanin biosynthesis in different sweet potato tissues.…”

Section: Identification Of R2r3-mybs Related To Anthocyanin Biosynthe...mentioning

confidence: 99%

Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Six Ipomoea Species and the Identification of Anthocyanin-Related Members in Sweet Potatoes

Zhou

et al. 2023

Plants

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Sweet potatoes (Ipomoea batatas) are one of the important tuberous root crops cultivated worldwide, and thier storage roots are rich in antioxidants, such as anthocyanins. R2R3-MYB is a large gene family involved in various biological processes, including anthocyanin biosynthesis. However, few reports about the R2R3-MYB gene family of sweet potatoes have been released to date. In the present study, a total of 695 typical R2R3-MYB genes were identified in six Ipomoea species, including 131 R2R3-MYB genes in sweet potatoes. A maximum likelihood phylogenetic analysis divided these genes into 36 clades, referring to the classification of 126 R2R3-MYB proteins of Arabidopsis. Clade C25(S12) has no members in six Ipomoea species, whereas four clades (i.e., clade C21, C26, C30, and C36), including 102 members, had no members in Arabidopsis, and they were identified as Ipomoea-specific clades. The identified R2R3-MYB genes were unevenly distributed on all chromosomes in six Ipomoea species genomes, and the collinearity analysis among hexaploid I. batatas and another five diploid Ipomoea species suggested that the sweet potato genome might have undergone a larger chromosome rearrangement during the evolution process. Further analyses of gene duplication events showed that whole-genome duplication, transposed duplication, and dispersed duplication events were the primary forces driving the R2R3-MYB gene family expansion of Ipomoea plants, and these duplicated genes experienced strong purifying selection because of their Ka/Ks ratio, which is less than 1. Additionally, the genomic sequence length of 131 IbR2R3-MYBs varied from 923 bp to ~12.9 kb with a mean of ~2.6 kb, and most of them had more than three exons. The Motif 1, 2, 3, and 4 formed typical R2 and R3 domains and were identified in all IbR2R3-MYB proteins. Finally, based on multiple RNA-seq datasets, two IbR2R3-MYB genes (IbMYB1/g17138.t1 and IbMYB113/g17108.t1) were relatively highly expressed in pigmented leaves and tuberous root flesh and skin, respectively; thus, they were identified to regulate tissue-specific anthocyanin accumulation in sweet potato. This study provides a basis for the evolution and function of the R2R3-MYB gene family in sweet potatoes and five other Ipomoea species.

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Transcript profiling for regulation of sweet potato skin color in Sushu8 and its mutant Zhengshu20

Cited by 13 publications

References 66 publications

Sizes, Components, Crystalline Structure, and Thermal Properties of Starches from Sweet Potato Varieties Originating from Different Countries

Sizes, Components, Crystalline Structure, and Thermal Properties of Starches from Sweet Potato Varieties Originating from Different Countries

Metabolomic and Transcriptomic Analyses of the Flavonoid Biosynthetic Pathway for the Accumulation of Anthocyanins and Other Flavonoids in Sweetpotato Root Skin and Leaf Vein Base

Genome-Wide Comparative Analysis of the R2R3-MYB Gene Family in Six Ipomoea Species and the Identification of Anthocyanin-Related Members in Sweet Potatoes

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