Transcription activation in cells lacking TAFIIs

Walker, Scott S.; Reese, Joseph C.; Apone, Lynne; Green, Michael R.

doi:10.1038/383185a0

Cited by 230 publications

(264 citation statements)

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“…TFIID plays a role in transducing the activation signal from upstream regulatory proteins to the basal machinery by directly interacting with transcriptional activators (Verrijzer and Tjian, 1996). Consistent with an important role for CCG1/TAF II 250 in G1 progression, a temperature-sensitive mutation in yTAF II 145, the homolog of CCG1/TAF II 250 in budding yeast, also causes cell cycle arrest in G1 at the restrictive temperature (Moqtaderi et al, 1996;Walker et al, 1996).…”

Section: Introductionmentioning

confidence: 94%

Overexpression of D-type cyclins, E2F-1, SV40 large T antigen and HPV16 E7 rescue cell cycle arrest of tsBN462 cells caused by the CCG1/TAFII250 mutation

Sekiguchi

Nishimoto

Hunter³

1999

Oncogene

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Japan tsBN462 cells, which have a point mutation in CCG1/ TAF II 250, a component of TFIID complex, arrest in G1 at the nonpermissive temperature of 39.58C. Overexpression of D-type cyclins rescued the cell cycle arrest of tsBN462 cells, suggesting that the cell cycle arrest was through Rb. Consistent with this, overexpression of E2F-1, whose function is repressed by the hypophosphorylated form of Rb, also rescued the cell cycle arrest. Moreover, expression of the viral oncoproteins SV40 large T antigen and HPV16 E7, which both bind Rb and inactivate its function, rescued the cell cycle arrest, whereas HPV16 E6 did not. Mutation of the Rb-binding motif in E7 abrogated its ability to rescue the cell cycle arrest. Expression of exogenous cyclin D1, SV40 large T antigen or CCG1/TAF II 250 increased cyclin A expression at 39.58C. Coexpression of HPV16 E7 and adenovirus E1b19K, which blocks apoptosis, rescued the proliferation of tsBN462 cells at 38.58C. To investigate the mechanism underlying the lack of cyclin D1 expression, deletion analysis of cyclin D1 promoter was performed. The 0.15 kbp cyclin D1 core promoter region, which lacks any transcription factor binding motifs, still exhibited a temperature-sensitive phenotype in tsBN462 cells suggesting that CCG1/TAF II 250 is critical for the function of the cyclin D1 core promoter.

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Section: Introductionmentioning

confidence: 94%

Overexpression of D-type cyclins, E2F-1, SV40 large T antigen and HPV16 E7 rescue cell cycle arrest of tsBN462 cells caused by the CCG1/TAFII250 mutation

Sekiguchi

Nishimoto

Hunter³

1999

Oncogene

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“…Cells were collected by centrifugation, washed and frozen on dry ice. RNA was isolated by glass bead disruption, as described in previous publications Walker et al, 1996). Northern blotting was performed (Sambrook et al, 1989;Apone et al, 1996) using the entire coding region of CLB2.…”

Section: Northern Blottingmentioning

confidence: 99%

“…Specificity of suppression. taf145-2 (Walker et al, 1996), tsm1/taf170 (Ray et al, 1991), taf17-2 and taf17-3 (Apone et al, 1998) mutant strains were transformed with the plasmids indicated in the centre of each panel. Ten-fold serial dilutions of saturated cultures were spotted onto plates and grown at 23uC or 37uC (taf145-2) or 37uC taf170/tsm1, taf17-2 and taf17-3).…”

Section: Taf68 and Cdc28 Mutations Are Synthetically Lethal In Combinmentioning

confidence: 99%

“…Mutation of five different yeast TAF II s genes resulted in a specific cell cycle arrest Walker et al, 1996;Green, 2000) and, likewise, mutation or depletion of four metazoan TAF II s also results in a similar phenotype (Green, 2000;Talavera and Basilico, 1977;Metzger et al, 1999;Martin et al, 1999;Aoyagi and Wassarman, 2000). Moreover, loss of function of mammalian TAF II s resulted in the arrest at the very same point in the cell cycle as their yeast counterparts, strongly indicating that the cell cycle regulatory functions of TAF II s have been well conserved.…”

Section: Introductionmentioning

confidence: 96%

“…Unexpectedly, initial studies revealed that the mutation or depletion of seven TAF II s individually resulted in gene-specific defects, and not global reductions in transcription Moqtaderi et al, 1996;Walker et al, 1996). In contrast, genetic analysis of other TFIID subunits that have sequence similarities to the core histones has shown that they are required for the transcription of a larger number of genes (Apone et al, 1998;Holstege et al, 1998;Michel et al, 1998;Moqtaderi et al, 1998;Natajaran et al, 1998;Sanders et al, 1999;Reese et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic analysis of TAF68/61 reveals links to cell cycle regulators

Reese

Green

2001

Yeast

Self Cite

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In yeast, inactivation of certain TBP-associated factors (TAF II s) results in arrest at specific stages of the cell cycle. In some cases, cell cycle arrest is not observed because overlapping defects in other cellular processes precludes the manifestation of an arrest phenotype. In the latter situation, genetic analysis has the potential to reveal the involvement of TAF II s in cell cycle regulation. In this report, a temperature-sensitive mutant of TAF68/61 was used to screen for high-copy dosage suppressors of its growth defect. Ten genes were isolated: TAF suppressor genes, TSGs 1-10. Remarkably, most TSGs have either a genetic or a direct link to control of the G 2 /M transition. Moreover, eight of the 10 TSGs can suppress a CDC28 mutant specifically defective for mitosis (cdc28-1N) but not an allele defective for passage through start. The identification of these genes as suppressors of cdc28-1N has identified four unreported suppressors of this allele. Moreover, synthetic lethality is observed between taf68-9 and cdc28-1N. The isolation of multiple genes involved in the control of a specific phase of the cell cycle argue that the arrest phenotypes of certain TAF II mutants reflect their role in specifically regulating cell cycle functions.

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A Review of Phenotypes in Saccharomyces cerevisiae

Hampsey¹

1997

Yeast

260

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A summary of previously defined phenotypes in the yeast Saccharomyces cerevisae is presented. The purpose of this review is to provide a compendium of phenotypes that can be readily screened to identify pleiotropic phenotypes associated with primary or suppressor mutations. Many of these phenotypes provide a convenient alternative to the primary phenotype for following a gene, or as a marker for cloning a gene by genetic complementation. In many cases a particular phenotype or set of phenotypes can suggest a function for the product of the mutated gene. © 1997 John Wiley & Sons, Ltd.

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Transcription activation in cells lacking TAFIIs

Cited by 230 publications

References 24 publications

Overexpression of D-type cyclins, E2F-1, SV40 large T antigen and HPV16 E7 rescue cell cycle arrest of tsBN462 cells caused by the CCG1/TAFII250 mutation

Overexpression of D-type cyclins, E2F-1, SV40 large T antigen and HPV16 E7 rescue cell cycle arrest of tsBN462 cells caused by the CCG1/TAFII250 mutation

Genetic analysis of TAF68/61 reveals links to cell cycle regulators

A Review of Phenotypes in Saccharomyces cerevisiae

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