Transcription factor accessibility and histone acetylation of the progesterone receptor gene differs between parental MCF-7 cells and a subline that has lost progesterone receptor expression

Xu, Xiaojie; Murdoch, Fern E.; Curran, Edward M.; Welshons, Wade V.; Fritsch, Michael K.

doi:10.1016/j.gene.2003.12.003

Cited by 18 publications

(12 citation statements)

References 24 publications

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“…Hence, it is not a surprise for us to find, in the present study, a unique set of genes (including AFT-3, caveolin-1, DLC-1, and NM23 H2) that are only upregulated by P4 in the OVCA cells but not in the HOSE cells. The ratios of PR-A to PR-B and transcription factor availability/accessibility differences may be the mechanistic basis for the observed differential regulation of these genes between normal and malignant cells (Giangrande et al, 2000;Xu et al, 2004). Lastly, it is worthy to note that the regulation of the said genes are not due to the recently cloned membrane PRs (Zhu et al, 2003), which utilize G proteins as transducers, since increases in the levels of their transcripts in OCa cells did not occur until 4 h (instead of minutes) after exposure to the progestin.…”

Section: Discussionmentioning

confidence: 99%

Identification of ATF-3, caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene profiling

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Identification of ATF-3, caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene profiling

et al. 2005

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“…The PRA promoter has a partial estrogen response element (ERE) that is important in mediating estrogen action in human breast cancer cells [58]. We and others have recently mapped the site of ER interaction within the PRB promoter in human breast cancer cells, as well as some of the complexities of regulation of these promoters [59][60][61][62]. The mouse and human PR promoters share some similarity in promoter sequences (especially in the PRA promoter), but are not identical.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Sauter

McDermid

Weinberg

et al. 2005

Experimental Cell Research

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The role of steroid hormone receptors in very early embryonic development remains unknown. Clearly, expression during organogenesis is important for tissue-specific development. However, progesterone receptor (PR) and estrogen receptors (ERα, ERβ), are expressed during early development through the blastocyst stage in mice and other species, and yet are not essential for embryonic viability. We have utilized the mouse embryonic stem (mES) cell model to investigate the regulated expression of these receptors during differentiation. Surprisingly, one of the earliest changes in gene expression in response to a differentiation signal observed is PR gene induction. It parallels the time course of expression for the patterning genes Hoxb1 and Hoxa5. Unexpectedly, PR gene expression is not regulated in an estrogen dependent manner by endogenous ERs or by transiently overexpressed ERα. Our results suggest a potentially novel mechanism of PR gene regulation within mES cells compared to adult tissues and the possibility of unique targets of PR action during early mES cell differentiation

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“…Chromatin Immunoprecipitation (ChIP)-ChIP was performed as previously described with minor alterations as detailed in the supplemental data (17). PCR amplification was performed on DNA recovered from the immunoprecipitation and the total chromatin input.…”

Section: Methodsmentioning

confidence: 99%

The Role of Histone Acetylation in Regulating Early Gene Expression Patterns during Early Embryonic Stem Cell Differentiation

McCool

Singer

et al. 2007

Journal of Biological Chemistry

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We have examined the role of histone acetylation in the very earliest steps of differentiation of mouse embryonic stem cells in response to withdrawal of leukemia inhibitory factor (LIF) as a differentiation signal. The cells undergo dramatic changes in morphology and an ordered program of gene expression changes representing differentiation to all three germ layers over the first 3-5 days of LIF withdrawal. We observed a global increase in acetylation on histone H4 and to a lesser extent on histone H3 over this time period. Treatment of the cells with trichostatin A (TSA), a histone deacetylase inhibitor, induced changes in morphology, gene expression, and histone acetylation that mimicked differentiation induced by withdrawal of LIF. We examined localized histone acetylation in the regulatory regions of genes that were transcriptionally either active in undifferentiated cells, induced during differentiation, or inactive under all treatments. There was striking concordance in the histone acetylation patterns of specific genes induced by both TSA and LIF withdrawal. Increased histone acetylation in local regions correlated best with induction of gene expression. Finally, TSA treatment did not support the maintenance or progression of differentiation. Upon removal of TSA, the cells reverted to the undifferentiated phenotype. We concluded that increased histone acetylation at specific genes played a role in their expression, but additional events are required for maintenance of differentiated gene expression and loss of the pluripotent state.

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Transcription factor accessibility and histone acetylation of the progesterone receptor gene differs between parental MCF-7 cells and a subline that has lost progesterone receptor expression

Cited by 18 publications

References 24 publications

Identification of ATF-3, caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene profiling

Identification of ATF-3, caveolin-1, DLC-1, and NM23-H2 as putative antitumorigenic, progesterone-regulated genes for ovarian cancer cells by gene profiling

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

The Role of Histone Acetylation in Regulating Early Gene Expression Patterns during Early Embryonic Stem Cell Differentiation

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