Transcription factor CDX2 directly regulates the expression of Ctenopharyngodon idellus intestinal PepT1 to mediate the transportation of oligopeptide

He, Zhou; Cai, Yuyang; Yang, Ming; Liu, Na; Zeng, Zihao; Li, Xiaojie; Zhang, Xiaomei; Liu, Suchun; Liu, Zhen

doi:10.1016/j.aqrep.2022.101148

Cited by 1 publication

(2 citation statements)

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“…Furthermore, the results found that GS would inhibit the expression of SP1, and MA would inhibit the expression of CDX2. A previous study showed that CDX2 regulated PepT1 via the assistance of SP1 in humans [ 54 ], while another study reported that CDX2 interacted with SP1 and could directly bind to the promoter of PepT1 to regulate its expression in grass carp [ 38 ]. These results indicate that the regulation of PepT1 by CDX2/SP1 was different between higher vertebrates and lower vertebrates.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, CDX2 was investigated to positively regulate PepT1 through SP1 as no CDX2 binding sites exist in the human PepT1 promoter, while it has binding sites of SP1. CDX2 interacted with SP1 and could not regulate the expression of PepT1 when the SP1 binding site on PepT1 was mutated [ 37 ], while CDX2 was investigated to bind the PepT1 promoter directly to regulate its transcription activity in grass carp [ 38 ]. According to the reference studies, the intestine genes CDX2, SP1, and PepT1 were regulated by butyrate, sodium butyrate, and tributyrin, while the tributyrin regulation pathway on PepT1 remains elusive.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Tributyrin on Growth Performance and Pathway by which Tributyrin Regulates Oligopeptide Transporter 1 in Juvenile Grass Carp (Ctenopharyngodon idellus)

Liu

Cai

et al. 2022

Animals

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The nutritional functions of tributyrin (TB) have been extensively studied, but questions remain regarding its influence on the growth of juvenile grass carp (Ctenopharyngodon idellus) and the regulation pathway to PepT1 in the intestine of grass carp. To answer the remaining questions, feeding trials, cell trials, and peritoneal injection trials were conducted in this study. The results showed that an appropriate level of TB (0.5 g/kg and 1.0 g/kg) supplementation in feed significantly promoted the growth performance of juvenile grass carp. The expressions of intestine genes (CDX2, SP1 and PepT1) related to oligopeptide transportation increased in the 0.5 g/kg TB group of feeding trials and both the 5 mM and 10 mM TB groups of the intestine cell trials, respectively. Subsequently, the injection trials of inhibitors CDX2 and SP1 demonstrated that the inhibition of CDX2 or SP1 decreased the mRNA expression of PepT1. Finally, the results of independent or combined treatments of TB and the inhibitors suggested that CDX2/SP1 mediated TB regulation on PepT1. These findings may help us to better understand the functions of TB on growth and PepT1 oligopeptide transportation, which could be modulated by dietary TB through the CDX2/SP1-PepT1 pathway in juvenile grass carp.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%