Transcription Factor ChbZIP1 from Alkaliphilic Microalgae Chlorella sp. BLD Enhancing Alkaline Tolerance in Transgenic Arabidopsis thaliana

Qu, Dehui; Show, Pau Loke; Miao, Xiangshui

doi:10.3390/ijms22052387

Cited by 11 publications

(9 citation statements)

References 45 publications

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“…It has been reported that GBF1 , the homolog in A. thaliana , was a negative regulator of blue light-dependent hypocotyl expansion ( Gangappa et al, 2013 ) and can trigger ROS accumulation ( Giri et al, 2017 , 1). ChbZIP1 that belongs to the G subfamily may enhance antioxidation by regulating genes related to oxidant detoxification in Alkaliphilic Microalgae Chlorella to adapt to abiotic stress ( Qu et al, 2021 ). These results suggested that the G subfamily of GubZIPs may respond to pathogen invasion and environmental stress factors by regulating the accumulation of ROS.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization and Expression Analysis of bZIP Gene Family Under Abiotic Stress in Glycyrrhiza uralensis

Han

Hou

et al. 2021

Front. Genet.

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bZIP gene family is one of the largest transcription factor families. It plays an important role in plant growth, metabolic, and environmental response. However, complete genome-wide investigation of bZIP gene family in Glycyrrhiza uralensis remains unexplained. In this study, 66 putative bZIP genes in the genome of G. uralensis were identified. And their evolutionary classification, physicochemical properties, conserved domain, functional differentiation, and the expression level under different stress conditions were further analyzed. All the members were clustered into 13 subfamilies (A–K, M, and S). A total of 10 conserved motifs were found in GubZIP proteins. Members from the same subfamily shared highly similar gene structures and conserved domains. Tandem duplication events acted as a major driving force for the evolution of bZIP gene family in G. uralensis. Cis-acting elements and protein–protein interaction networks showed that GubZIPs in one subfamily are involved in multiple functions, while some GubZIPs from different subfamilies may share the same functional category. The miRNA network targeting GubZIPs showed that the regulation at the transcriptional level may affect protein–protein interaction networks. We suspected that domain-mediated interactions may categorize a protein family into subfamilies in G. uralensis. Furthermore, the tissue-specific gene expression patterns of GubZIPs were analyzed using the public RNA-seq data. Moreover, gene expression level of 66 bZIP family members under abiotic stress treatments was quantified by using qRT-PCR. The results of this study may serve as potential candidates for functional characterization in the future.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Characterization and Expression Analysis of bZIP Gene Family Under Abiotic Stress in Glycyrrhiza uralensis

Han

Hou

et al. 2021

Front. Genet.

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“…BLD has been reported to increase the alkali resistance of plants. Overexpression of ChbZIP1 in Arabidopsis showed that ChbZIP1 can enhance plant adaptation to alkali stress through the active oxygen detoxification pathway, suggesting its promising potential in genetically improving plant tolerance to alkali stress (Qu et al, 2021). bZIP transcription factors are similar to MYB/MYC transcription factors in terms of their regulation, participation in ABA-dependent pathway signal transduction, and perception of stress signals to regulate gene expression.…”

Section: Induction Of Transcription Factor Expressionmentioning

confidence: 99%

Response Mechanisms of Plants Under Saline-Alkali Stress

2021

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As two coexisting abiotic stresses, salt stress and alkali stress have severely restricted the development of global agriculture. Clarifying the plant resistance mechanism and determining how to improve plant tolerance to salt stress and alkali stress have been popular research topics. At present, most related studies have focused mainly on salt stress, and salt-alkali mixed stress studies are relatively scarce. However, in nature, high concentrations of salt and high pH often occur simultaneously, and their synergistic effects can be more harmful to plant growth and development than the effects of either stress alone. Therefore, it is of great practical importance for the sustainable development of agriculture to study plant resistance mechanisms under saline-alkali mixed stress, screen new saline-alkali stress tolerance genes, and explore new plant salt-alkali tolerance strategies. Herein, we summarized how plants actively respond to saline-alkali stress through morphological adaptation, physiological adaptation and molecular regulation.

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“…We constructed a large-scale screening system with rice seedlings by 140 mM Na-HCO 3 (pH 9.20) treatment [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ] in a greenhouse. The large-scale screening system was constructed by modifying the method of Bado et al (2020) [ 50 ] ( Figure S1A–D ).…”

Section: Methodsmentioning

confidence: 99%

“…In fact, most reported CSAS tolerance mechanisms in plants are similar to salt stress or alkali stress tolerance mechanisms, such as accumulating organic acids to bring down the pH around the roots and inhibit the precipitation of metal ions and phosphate [ 4 , 5 ], accumulating more osmolytes (proline, soluble sugars, betaine and soluble proteins) to adjust osmosis balance [ 6 ], increasing the activity of antioxidant enzymes (catalase, superoxide dismutase, peroxidase and ascorbate peroxidase) to detoxify reactive oxygen species (ROS) [ 6 , 7 , 8 ], and regulating stomatal closure through ABA-induced pathway [ 9 , 10 ]. Many related studies for saline–alkali stress are performed using NaHCO 3 stress on the species including Salix linearistipularis , Chlorella vulgaris , Arabidopsis thaliana , Glycine soja and Medicago sativa [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. However, quite a few studies were focused on CSAS in monocots such as Puccinellia tenuiflora , sorghum and rice [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Matrix Polysaccharides Contribute to Salt–Alkali Tolerance in Rice

Liu

et al. 2022

IJMS

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Salt–alkali stress threatens the resilience to variable environments and thus the grain yield of rice. However, how rice responds to salt–alkali stress at the molecular level is poorly understood. Here, we report isolation of a novel salt–alkali-tolerant rice (SATR) by screening more than 700 germplasm accessions. Using 93-11, a widely grown cultivar, as a control, we characterized SATR in response to strong salt–alkali stress (SSAS). SATR exhibited SSAS tolerance higher than 93-11, as indicated by a higher survival rate, associated with higher peroxidase activity and total soluble sugar content but lower malonaldehyde accumulation. A transcriptome study showed that cell wall biogenesis-related pathways were most significantly enriched in SATR relative to 93-11 upon SSAS. Furthermore, higher induction of gene expression in the cell wall matrix polysaccharide biosynthesis pathway, coupled with higher accumulations of hemicellulose and pectin as well as measurable physio-biochemical adaptive responses, may explain the strong SSAS tolerance in SATR. We mapped SSAS tolerance to five genomic regions in which 35 genes were candidates potentially governing SSAS tolerance. The 1,4-β-D-xylan synthase gene OsCSLD4 in hemicellulose biosynthesis pathway was investigated in details. The OsCSLD4 function-disrupted mutant displayed reduced SSAS tolerance, biomass and grain yield, whereas the OsCSLD4 overexpression lines exhibited increased SSAS tolerance. Collectively, this study not only reveals the potential role of cell wall matrix polysaccharides in mediating SSAS tolerance, but also highlights applicable value of OsCSLD4 and the large-scale screening system in developing SSAS-tolerant rice.

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Transcription Factor ChbZIP1 from Alkaliphilic Microalgae Chlorella sp. BLD Enhancing Alkaline Tolerance in Transgenic Arabidopsis thaliana

Cited by 11 publications

References 45 publications

Genome-Wide Characterization and Expression Analysis of bZIP Gene Family Under Abiotic Stress in Glycyrrhiza uralensis

Genome-Wide Characterization and Expression Analysis of bZIP Gene Family Under Abiotic Stress in Glycyrrhiza uralensis

Response Mechanisms of Plants Under Saline-Alkali Stress

Cell Wall Matrix Polysaccharides Contribute to Salt–Alkali Tolerance in Rice

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