Transcription factor ERF016 regulates vascular structure and water metabolism to enhance drought tolerance in poplar

Zou, Shengqiang; Xu, Zhi-Fang; Huan, Xuhui; Hu, Jia; Zhou, Lieding; Jin, Xin; Zhao, Kai; Han, Youzhi; Wang, Shengji

doi:10.1007/s10725-022-00956-0

Cited by 3 publications

(2 citation statements)

References 50 publications

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“…MDA content was determined using the colorimetric method using barbituric acid. Root vitality was determined using the triphenyltetrazolium chloride (TTC) method [56,57]. At least six biological replicates were performed for each experiment.…”

Section: Determination Of Physiological Indicatorsmentioning

confidence: 99%

PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation

Zhou,

Huan,

Zhao

et al. 2023

IJMS

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Salt stress is one of the major abiotic stresses that limits plant growth and development. The MYB transcription factor family plays essential roles in plant growth and development, as well as stress tolerance processes. In this study, the cDNA of the 84K poplar (Populus abla × Populus glandulosa) was used as a template to clone the full length of the PagMYB205 gene fragment, and transgenic poplar lines with PagMYB205 overexpression (OX) or inhibited expression (RNAi, RNA interference) were cultivated. The role of PagMYB205 in poplar growth and development and salt tolerance was detected using morphological and physiological methods. The full-length CDS sequence of PagMYB205 was 906 bp, encoding 301 amino acids, and the upstream promoter sequence contained abiotic stress-related cis-acting elements. The results of subcellular localization and transactivation assays showed that the protein had no self-activating activity and was localized in the nucleus. Under salt stress, the rooting rate and root vitality of RNAi were higher than OX and wild type (WT). However, the malondialdehyde (MDA) content of the RNAi lines was significantly lower than that of the wild-type (WT) and OX lines, but the reactive oxygen species (ROS) scavenging ability, such as the peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) enzyme activities, was dramatically more powerful. Most significantly of all, the RNAi3 line with the lowest expression level of PagMYB205 had the lowest MDA content, the best enzyme activity and root vitality, and the best salt stress tolerance compared to the other lines. The above results suggest that the transcription factor PagMYB205 could negatively regulate salt stress tolerance by regulating antioxidant enzyme activity and root vitality.

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Section: Determination Of Physiological Indicatorsmentioning

confidence: 99%

PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation

Zhou,

Huan,

Zhao

et al. 2023

IJMS

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“…In addition, PtabZIP1L, homologous to Arabidopsis bZIP1, has been reported to be a positive regulator of lateral root growth and drought tolerance in poplar [ 19 ]. The overexpression of ERF016 in 84K poplar ( Populus alba × P. glandulosa ) enhanced root drought tolerance by increasing the number of lateral roots and other characteristics [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

PtrABR1 Increases Tolerance to Drought Stress by Enhancing Lateral Root Formation in Populus trichocarpa

Sun,

Dong,

Song

2023

IJMS

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Roots are the main organ for water uptake and the earliest part of a plant’s response to drought, making them of great importance to our understanding of the root system’s response to drought. However, little is known about the underlying molecular mechanisms that control root responses to drought stress. Here, we identified and functionally characterized the AP2/ERF family transcription factor (TF) PtrABR1 and the upstream target gene zinc-finger protein TF PtrYY1, which respond to drought stress by promoting the growth and development of lateral roots in Populus trichocarpa. A root-specific induction of PtrABR1 under drought stress was explored. The overexpression of PtrABR1 (PtrABR1-OE) promoted root growth and development, thereby increasing tolerance to drought stress. In addition, PtrYY1 is directly bound to the promoter of PtrABR1 under drought stress, and the overexpression of PtrYY1 (PtrYY1-OE) promoted lateral root growth and development and increased tolerance to drought stress. An RNA-seq analysis of PtrABR1-OE with wild-type (WT) poplar identified PtrGH3.6 and PtrPP2C44, which share the same pattern of expression changes as PtrABR1. A qRT-PCR and cis-element analysis further suggested that PtrGH3.6 and PtrPP2C44 may act as potential downstream targets of PtrABR1 genes in the root response pathway to drought stress. In conclusion, these results reveal a novel drought regulatory pathway in which PtrABR1 regulates the network through the upstream target gene PtrYY1 and the potential downstream target genes PtrGH3.6 and PtrPP2C44, thereby promoting root growth and development and improving tolerance to drought stress.

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PagbZIP75 decreases the ROS accumulation to enhance salt tolerance of poplar via the ABA signaling

Hu,

Nan,

Zhou

et al. 2024

Environmental and Experimental Botany

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Transcription factor ERF016 regulates vascular structure and water metabolism to enhance drought tolerance in poplar

Cited by 3 publications

References 50 publications

PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation

PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation

PtrABR1 Increases Tolerance to Drought Stress by Enhancing Lateral Root Formation in Populus trichocarpa

PagbZIP75 decreases the ROS accumulation to enhance salt tolerance of poplar via the ABA signaling

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