Transcription factor Nkx2-3 maintains the self-renewal of hematopoietic stem cells by regulating mitophagy

Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells

“…Bulk RNA-seq revealed that hPSC-derived day-10 hematopoietic progenitors and human embryonic DA CD144+ CD45+ HSCs 143 expressed comparable levels of HSC signature transcription factors, including HLF , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 HOPX , 160 NKX2.3 , 161 MEIS1 , 162 , 163 , 164 , 165 and HOXA genes ( HOXA5 , HOXA9 , and HOXA10 ) 62 , 65 , 74 ( Figures 6 B and S7 C). As a negative control, human DA CD144− CD45+ non-HSCs minimally expressed these HSC signature transcription factors ( Figures 6 B and S7 C).…”