Transcription factor Sp1 recognizes a DNA sequence in the mouse dihydrofolate reductase promoter

Dynan, William S.; Sazer, Shelley; Tjian, Robert; Schimke, Robert T.

doi:10.1038/319246a0

Cited by 272 publications

(150 citation statements)

References 21 publications

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“…The promoter region contains two Sp-1 sites at 757 and +87 at close proximity to CAP sites and another site at position 7508. The Sp-1 sequences have been implicated in transcriptional activation of TATA-less promoters present in housekeeping genes and proto-oncogenes (Dynan et al, 1986). Multiple purine rich GGAA Ets Binding Sites (EBS) were identi®ed (at positions 7653, 7349 and 7278) in the human Fli-1b promoter .…”

Section: Promoter Elementsmentioning

confidence: 99%

Fli-1b is generated by usage of differential splicing and alternative promoter

et al. 1998

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The proto-oncogene Fli-1, a member of Ets family is rearranged or activated through proviral integration in erythroleukemias, induced by Friends' Murine Leukemia Virus. The DNA binding domain (ETS domain) of Fli-1 is fused to the RNA binding domain of EWS by t(11q24:22q12) chromosomal translocation in Ewing's sarcoma and primitive neuroectodermal tumors. Screening of human cDNA libraries has identi®ed two di erent 5'-termini and alternatively spliced forms of the human Fli-1 gene (Fli-1b), suggesting the possible existence of two independent promoters. The genomic sequence adjacent to the alternate exon of human Fli-1b gene shows functional promoter activity when cloned in promoter-less CAT expression vector and transfected into QT-6 cells. The transcription initiation (CAP) site and minimum promoter region necessary for function were localized. The 5'-¯anking regions of human Fli-1b and mouse Fli-1 show 80% homology suggesting conserved promoter regulatory elements. The Fli-1b 5'-anking sequence lacks canonical TATA or CCAAT boxes but contains a partially conserved TATA-like sequence at position 242. Several transcription factor binding sequences like ATF/CREB, E2A-PBX1, EBP, PEA-3, ETS-2, Sp-1, c-Myc, TBP, GATA-1 and Oct-3 were conserved in the promoter sequence. Functional promoter assays revealed that Fli-1b promoter shows very strong transcriptional activation compared to Fli-1 promoter. We also showed that variant Fli-1b has transcriptional activation properties similar to those of Fli-1. Fli-1b and Fli-1 show di erential expression in various hematopoietic cell lines. This di erential expression and promoter activities of Fli-1 and Fli-1b suggests that several mechanisms are involved in Fli-1 gene regulation which are mediated by many transcription factors.

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Section: Promoter Elementsmentioning

confidence: 99%

Fli-1b is generated by usage of differential splicing and alternative promoter

et al. 1998

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“…These genes do, however, contain one or more copies of the sequence GGGCGG or its inverse complement CCGCCC upstream from their transcription start point. This sequence is found four times in the mouse DHFR promoter (Dynan et al, 1986) and has been shown to be an important component of the SV40 virus early promoter (BarreraSladana et al, 1985) as well as the thymidine kinase promoter of Herpes simplex virus (McKnight et al, 1984). Examination of the sequence in Fig.…”

Section: (B) Analysis Of the Promoter Regionmentioning

confidence: 99%

Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene

Fouts

Ganguly

Gutierrez

et al. 1988

Gene

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“…The 5'-flanking region of exon 1 contained no potential CCAAT and TATA sequences, but contained three potential binding sites for the transcription factor Sp1 (CCGCCC) (Fig. 2A, open box) [9]. A potential binding site for Sp1 was also present at nucleotide position +297 ( Fig.…”

Section: Dna Sequencing and Mapping Of The 5'non-coding Region Of Thementioning

confidence: 99%

Characterization of the Bovine Prion Protein Gene: The Expression Requires Interaction between the Promoter and Intron.

Inoue

Tanaka

Horiuchi

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. We cloned the part of the bovine PrP gene which contains the 5'-flanking region, exon 1, exon 2 and intron 1 to analyze its promoter region. The 5' non-coding region of the bovine PrP gene consisted of three exons and two introns, and its organization was similar to that of the mouse, rat and sheep PrP genes. The 5'-flanking region of the bovine PrP gene from the transcription start site to nucleotide position -88 was (G+C)-rich (78%) and contained three potential binding sites for the transcription factor Sp1, but no CCAATbox or TATA-box. This region showed high homology (89%) with that of the sheep PrP gene, but relatively low homology (approximately 46-62%) with the same region of the mouse, rat, hamster and human PrP genes. The position from -88 to -30 within the 5'-flanking region of the bovine PrP gene showed major promoter activity. However, this region was able to function properly only in collaboration with the region at +123 to +891 of intron 1 of the bovine PrP gene. -KEY WORDS: bovine PrP, intron, prion protein gene, promoter.

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Transcription factor Sp1 recognizes a DNA sequence in the mouse dihydrofolate reductase promoter

Cited by 272 publications

References 21 publications

Fli-1b is generated by usage of differential splicing and alternative promoter

Fli-1b is generated by usage of differential splicing and alternative promoter

Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene

Characterization of the Bovine Prion Protein Gene: The Expression Requires Interaction between the Promoter and Intron.

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