Transcription, Translation, and Cellular Localization of ThreeAutographa californicaNuclear Polyhedrosis Virus Structural Proteins: ODV-E18, ODV-E35, and ODV-EC27

Abstract

This paper identifies two structural proteins of the occluded derived viral envelope of Autographa californica nuclear polyhedrosis virus (AcMNPV): ODV-E18 and ODV-E35. In addition, we identify a protein, ODV-EC27, that is incorporated into the capsid of occluded virus, which is not detected in budded virus. The genes for these proteins reside within the IE0 intron. The intron was sequenced, and five open reading frames (ORF) were identified. ORF 3 (genomic ORF 143) codes for the ODV envelope protein, ODV-E18. ORF 4 (genomic ORF 144) codes for ODV-EC27, and Western blot analyses locate this protein to both the ODV capsid and envelope. Transcripts for both ODV-E18 and ODV-EC27 initiate from conserved TAAG motifs, and transcripts are detected from 16 through 72 hr p.i. Antiserum to ODV-E18 recognizes a band of 18 kDa on Western blots of extracts from infected cells and bands of 18 and 35 kDa on Western blots of proteins from purified ODV envelope. N-terminal amino acid sequencing reveals that both ODV-E18 and ODV-E35 contain the same N-terminus. Antiserum to ODV-EC27 recognizes a protein of 27 kDa on Western blots of extracts from infected cells and bands of 27 and 35 kDa on Western blots of proteins from purified ODV. Using immunogold labeling techniques, ODV-E18 and/or ODV-E35 are detected in viral induced intranuclear microvesicles and are not detected in the plasma membrane, cytoplasmic membranes, or the nuclear envelope. Immunogold labeling using antisera to ODV-EC27 detects this protein on both the ODV envelope and capsid.

Autographa californicaNucleopolyhedrovirus Ac76: a Dimeric Type II Integral Membrane Protein That Contains an Inner Nuclear Membrane-Sorting Motif

“…According to the heptad repeat model (abcdefg) n in the TM helix of phospholamban, Leu 37 , Leu 44 , and Leu 51 occupy the a position and Ile 40 and Ile 47 occupy the d position in the motif; these residues create a leucine/isoleucine zipper (34,40). The leucine-and isoleucine-rich sequence 26 LYLLILFLV FIIVSPAII 43 in the TM helix of Ac76 shows similar characteristics, as L 26 and L 33 are localized to position a and L 29 , I 36 , and I 43 are localized to position d (Fig. 2E); thus, this sequence likely forms a hpt, the cells were incubated either with 20 g/ml digitonin for semipermeabilization or with 0.25% Triton X-100 for full permeabilization.…”

Identification, Characterization and Phylogenic Analysis of Conserved Genes within the odvp-6e/odv-e56 Gene Region of Choristoneura fumiferana Granulovirus

“…ORF coding for this protein were detected on the genome of several members of the Baculoviridae family. ODV-E18 has been characterized as a late protein in AcMNPV that is associated with both the ODV envelope and capsid (Braunagel et al, 1996). The ORF number 12 on the ChfuGV genome potentially codes for a ODV-E18 homologue.…”

Identification and Characterization of a Conserved Baculoviral Structural Protein ODVP-6E/ODV-E56 from Choristoneura fumiferana Granulovirus

“…Tel: 204-230-0210;Fax: 204-237-3240 E-mail: kiarashidan@juno.com al., 1996). The invaginated-inner nuclear membrane has been hypothesized to be the source of the baculovirus-induced intracellular microvesicles (Braunagel et al, 1996). It has been shown that one of the ODV-specific envelope proteins that can be found in these intracellular microvesicle structures is ODVP-6E/ODV-E56 (Braunagel et al, 1996).…”

“…Among these structural proteins, at least eight have been known to be specific to the ODV phenotype. These are as follows: VP17 (Funk and Consigli, 1993), ODV-E25 (Russell and Rohrmann, 1993), ODV-E35 (Braunagel et al, 1996), GP41 (Whitford and Faulkner, 1993), P74 (Kuzio et al, 1089), ODV-E18 (Braunagel et al, 1996), ODV-E66 (Hong et al, 1994), and ODVP-6E/ODV-E56 (Braunagel et al, 1996, Theilmann et al, 1996. The proteins that could participate in adsorption, fusion, and penetration have not been identified.…”