Transcriptional dynamics of immune, growth and stress related genes in skeletal muscle of the fine flounder ( Paralichthys adpersus) during different nutritional statuses

Valenzuela, Cristina Ofelia; Escobar, Daniela; Pérez, Luis; Zuloaga, Rodrigo; Estrada, Juan Manuel; Mercado, Luís; Valdés, Juan Antonio; Molina, Alfredo

doi:10.1016/j.dci.2015.06.007

Cited by 18 publications

(8 citation statements)

References 45 publications

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“…These data are in line with studies in rat muscle cells treated with corti- costeroid (52) or associated with nutritional stress (28), which showed increasing klf15 and redd1 expression in a GR-dependent manner. Previously in fine flounder, klf15 and redd1 were shown to increase in muscle atrophy induced by nutritional stress (49). Furthermore, it has been recently reported that both klf15 and redd1 exhibited differential expression depending on fish size, being significantly higher in small rainbow trout compared with larger individuals (8).…”

Section: Discussionmentioning

confidence: 99%

“…The ubiquitin-proteasome pathway promotes protein degradation via protein polyubiquitination that are recognized and degraded by the 26S proteasome (32). Recent studies in fish have correlated the effects of stress with skeletal muscle atrophy (1,49). In agreement with these data, protein degradation by the ubiquitin-proteasome system has also been described during stress by starvation (fine flounder and rainbow trout) (16,42), high and low temperature (Antarctic eelpout) (54) and handling (red cusk-eel) (1), suggesting a main role during stress response in regulating muscle growth.…”

Section: Chronic Stress Induced By Crowding Induces Muscle Proteolytic Pathways Through Different Nonoverlapped Mechanismsmentioning

confidence: 99%

“…In teleost fish as well as other vertebrates, skeletal muscle represents mostly the body mass, and its growth depends on the balance between anabolic and catabolic processes (22), which are affected by stress (49). The main promoter of muscle growth is the endocrine system, through the growth hormone and insulin-like growth factor (GH/IGF) system.…”

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confidence: 99%

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Chronic stress inhibits growth and induces proteolytic mechanisms through two different nonoverlapping pathways in the skeletal muscle of a teleost fish

Valenzuela

Zuloaga

Mercado

et al. 2018

American Journal of Physiology-Regulatory, Integrative and Comp

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Chronic stress detrimentally affects animal health and homeostasis, with somatic growth, and thus skeletal muscle, being particularly affected. A detailed understanding of the underlying endocrine and molecular mechanisms of how chronic stress affects skeletal muscle growth remains lacking. To address this issue, the present study assessed primary (plasma cortisol), secondary (key components of the GH/IGF system, muscular proteolytic pathways, and apoptosis), and tertiary (growth performance) stress responses in fine flounder ( Paralichthys adspersus) exposed to crowding chronic stress. Levels of plasma cortisol, glucocorticoid receptor 2 ( gr2), and its target genes ( klf15 and redd1) mRNA increased significantly only at 4 wk of crowding ( P < 0.05). The components of the GH/IGF system, including ligands, receptors, and their signaling pathways, were significantly downregulated at 7 wk of crowding ( P < 0.05). Interestingly, chronic stress upregulated the ubiquitin-proteasome pathway and the intrinsic apoptosis pathways at 4wk ( P < 0.01), whereas autophagy was only significantly activated at 7 wk ( P < 0.05), and meanwhile the ubiquitin-proteasome and the apoptosis pathways returned to control levels. Overall growth was inhibited in fish in the 7-wk chronic stress trial ( P < 0.05). In conclusion, chronic stress directly affects muscle growth and downregulates the GH/IGF system, an action through which muscular catabolic mechanisms are promoted by two different and nonoverlapping proteolytic pathways. These findings provide new information on molecular mechanisms involved in the negative effects that chronic stress has on muscle anabolic/catabolic signaling balance.

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Section: Discussionmentioning

confidence: 99%

Section: Chronic Stress Induced By Crowding Induces Muscle Proteolytic Pathways Through Different Nonoverlapped Mechanismsmentioning

confidence: 99%

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Chronic stress inhibits growth and induces proteolytic mechanisms through two different nonoverlapping pathways in the skeletal muscle of a teleost fish

Valenzuela

Zuloaga

Mercado

et al. 2018

American Journal of Physiology-Regulatory, Integrative and Comp

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“…However, in fish such as rainbow trout this system is responsible for only 17% of the protein degradation of myotubes [ 9 ]. The two specific markers for UPS in fish muscle are known as MurRF1 (Muscle RING-finger protein 1) and Atrogin1 [ 10 , 11 ]. On the other hand, the ALS is responsible for more than 30% of total protein degradation of the skeletal muscle cells in fish [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)

et al. 2020

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Background: Skeletal muscle is one of the tissues most affected by stress conditions. The protein degradation in this tissue is vital for the supply of energy mediated by different proteolytic pathways such as the ubiquitinproteasome (UPS), autophagy-lysosome (ALS) and the calpain/calpastatin system (CCS). Nevertheless, the regulation of this proteolytic axis under stress conditions is not yet completely clear. Chile is the main producer of rainbow trout (Oncorhynchus mykiss) in the world. This intensive fish farming has resulted in growing problems as crowding and stress are one of the major problems in the freshwater stage. In this context, we evaluated the crowding effect in juvenile rainbow trout kept in high stocking density (30 kg/m 3) for 15, 45 and 60 days, using a control group of fish (10 kg/m 3). Results: Plasmatic cortisol and glucose were evaluated by enzyme immunoassay. The mRNA levels of stress-related genes (gr1, gr2, mr, hsp70, klf15 and redd1), markers of the UPS (atrogin1 and murf1) and CCS (capn1, capn1, cast-l and casts) were evaluated using qPCR. ALS (LC3-I/II and P62/SQSTM1) and growth markers (4E-BP1 and ERK) were measured by Western blot analysis. The cortisol levels increased concomitantly with weight loss at 45 days of crowding. The UPS alone was upregulated at 15 days of high stocking density, while ALS activation was observed at 60 days. However, the CCS was inactivated during the entire trial. Conclusion: All these data suggest that stress conditions, such as crowding, promote muscle degradation in a time-dependent manner through the upregulation of the UPS at early stages of chronic stress and activation of the ALS in long-term stress, while the CCS is strongly inhibited by stress conditions in the rainbow trout muscle farmed during freshwater stage. Our descriptive study will allow perform functional analysis to determine, in a more detailed way, the effect of stress on skeletal muscle physiology as well as in the animal welfare in rainbow trout. Moreover, it is the first step to elucidate the optimal crop density in the freshwater stage and improve the standards of Chilean aquaculture.

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“…In contrast, the expression of ER stress-related proteins XBP1, GRP78 and p38/JNK and FOXO1 were signi cantly up-regulated, indicating that H 2 S exposure activated p38/JNK/by up-regulating PKC-δ FOXO1 axis. FOXO1 plays an important role in autophagy (Kishino et al, 2017), inducing autophagy and cell death through the BNIP3 and mTOR pathways Valenzuela et al, 2015). In this study, we observed that the increase in FOXO1 expression activated the BNIP3 and mTOR pathways and that the autophagy marker LC3II was activated after H 2 S exposure.…”

Section: Discussionmentioning

confidence: 65%

H2S Exposure Induces Autophagy Injury in The Jejunum of Broilers Through miR-181a-PKC-δ Axis Mediated ER Stress

Zhu

Guo

Chen

et al. 2021

Preprint

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Background:Hydrogen sulfide (H2S), as a toxic atmospheric and industrial pollutant, is one of the main components of haze. Air exposure to H2S can cause multiple organ and tissue damage. However, studies on the animal intestines of H2S toxicity are limited. To clarify the effect of H2S exposure on intestinal proteomics and the role of autophagy in intestinal injury, in the present study, effects of H2S exposure on jejunum damage of broilers were examined by establishing a 42-day H2S exposure broiler model. Results:We observed the morphological changes that arose, screened differentially expressed proteins (DEPS) and enriched related pathways via electron microscopy, proteomics and bioinformatics analyses, qRT-PCR, and western blot. We obtained 86 proteins that were significantly different under H2S exposure, including proteins related to autophagy and ER stress regulation. Meanwhile, protein-transcriptomics combined analysis found that autophagy and ER stress-associated pathways MAPK signaling pathway, FOXO signaling pathway, mTOR signaling pathway and Autophagy were significantly enriched. Besides, the expression of the autophagy-related indicators LC3II/I, Dynein, Beclin1, BNIP3, and ULK1 were remarkably up-regulated under H2S exposure (P < 0.05). In contrast, the activities of p62 and mTOR were significantly decreased (P < 0.05). Conclusions: The above results indicate that H2S induced autophagy in the jejunum via the miR-181a-PKC-δ axis activates ER stress to induce p38/JNK expression. In our study, the combined analysis of proteomics and transcriptomics on broiler intestines was described for the first time and verified the selected pathways related to ER stress and autophagy. These results provide novel insights into the toxicology of H2S and environmental research.

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Transcriptional dynamics of immune, growth and stress related genes in skeletal muscle of the fine flounder ( Paralichthys adpersus) during different nutritional statuses

Cited by 18 publications

References 45 publications

Chronic stress inhibits growth and induces proteolytic mechanisms through two different nonoverlapping pathways in the skeletal muscle of a teleost fish

Chronic stress inhibits growth and induces proteolytic mechanisms through two different nonoverlapping pathways in the skeletal muscle of a teleost fish

Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)

H2S Exposure Induces Autophagy Injury in The Jejunum of Broilers Through miR-181a-PKC-δ Axis Mediated ER Stress

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Transcriptional dynamics of immune, growth and stress related genes in skeletal muscle of the fine flounder ( Paralichthys adpersus) during different nutritional statuses

Cited by 18 publications

References 45 publications

Chronic stress inhibits growth and induces proteolytic mechanisms through two different nonoverlapping pathways in the skeletal muscle of a teleost fish

Chronic stress inhibits growth and induces proteolytic mechanisms through two different nonoverlapping pathways in the skeletal muscle of a teleost fish

Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)

H2S Exposure Induces Autophagy Injury in The Jejunum of Broilers Through miR-181a-PKC-δ Axis Mediated ER&nbsp;Stress

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H2S Exposure Induces Autophagy Injury in The Jejunum of Broilers Through miR-181a-PKC-δ Axis Mediated ER Stress