Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress

Hagiwara, Daisuke; Asano, Yoshihiro; Marui, Junichiro; Yoshimi, Akira; Mizuno, Takeshi; Abe, Keietsu

doi:10.1016/j.fgb.2009.07.003

Cited by 70 publications

(83 citation statements)

References 76 publications

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“…In the treated B+ line processes related to MAPK signal transduction (such as phosphorus and phosphate metabolism categories, protein kinase cascade and phosphorylation processes) were overall up-regulated, while they were down-regulated in the treated B-line. Since the whole MAPK cascade is activated by diverse environmental stresses as a powerful actor to control gene expression and conferring cellular stress resistance (Morigasaki et al 2013;Hagiwara et al 2009), we hypothesize that the fungus with its endobacterium may more efficiently face oxidative stress, as well as high osmolarity and heat shock. Also DNA conformation/packaging GO categories were upregulated in the B+ treated line only.…”

Section: The Fungal Responses To H2o2 Change Depending On the Presencmentioning

confidence: 99%

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

et al. 2017

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Arbuscular mycorrhizal (AM) fungi experience oxidative stress during the plant-fungal interaction, due to endogenous reactive oxygen species (ROS) produced by fungal metabolism and exogenous ROS produced by plant cells. Here, we examine the responses to H2O2 in Gigaspora margarita, an AM fungus containing the endobacterial symbiont Candidatus Glomeribacter gigasporarum (CaGg). Previous studies revealed that G. margarita with its endobacterium produces more ATP and has higher respiratory activity compared to a cured line that lacks the endobacterium. This higher bioenergetic potential leads to higher production of ROS, and to a higher ROS-detoxifying capacity, suggesting a direct or indirect role of the endobacterium in modulating fungal anti-oxidant responses. To test the hypothesis that the fungal-endobacterial symbiosis may enhance the fitness of the AM fungus in the presence of oxidative stress, we treated the fungus with a sublethal concentration of H2O2 and performed RNA-seq analysis. Our results demonstrate that: i) irrespective of the endobacterium presence, G. margarita faces oxidative stress by activating multiple metabolic processes (methionine oxidation, sulfur uptake, the pentose phosphate pathway, activation of ROS-scavenger genes), ii) in the presence of its endobacterium, G. margarita upregulates some metabolic pathways, like chromatin status modifications and iron metabolism; iii) contrary to our hypothesis, the cured line responds to H2O2 by activating the transcription of specific ROS scavengers. We confirmed the RNA-seq findings by measuring the glutathione and ascorbate content, which was the same in both lines after H2O2 treatment. We conclude that both fungal lines

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Section: The Fungal Responses To H2o2 Change Depending On the Presencmentioning

confidence: 99%

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

et al. 2017

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“…This finding was supported by Ochiai et al [43] who found that fludioxonil can disturb the CANIKI/COSI signal transduction pathway, leading to the dysfunction of glycerol synthesis and inhibition of hyphae formation in Candida albicans. Recently, Hagiwara et al [44] reported the inhibiting effect of fludioxonil on a large number of genes involved in a two-component signal transduction system, in filamentous fungi. Impact on this system suggests that fludioxonil may have a nontarget effect on bacteria, as this dualistic signal transduction mechanisms is also reported in prokaryotes [45].…”

Section: Effects On Signal Transductionmentioning

confidence: 99%

Fungicide: Modes of Action and Possible Impact on Nontarget Microorganisms

et al. 2011

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Fungicides have been used widely in order to control fungal diseases and increase crop production. However, the effects of fungicides on microorganisms other than fungi remain unclear. The modes of action of fungicides were never well classified and presented, making difficult to estimate their possible nontarget effects. In this paper, the action modes and effects of fungicides targeting cell membrane components, protein synthesis, signal transduction, respiration, cell mitosis, and nucleic acid synthesis were classified, and their effects on nontarget microorganisms were reviewed. Modes of action and potential non-target effects on soil microorganisms should be considered in the selection of fungicide in order to protect the biological functions of soil and optimize the benefits derived from fungicide use in agricultural systems.

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“…SakA interacts with the b-Zip transcription factor AtfA, which activates the catalase genes catA and catB during oxidative stress (25) and both ⌬sakA and ⌬atfA mutants are sensitive to oxidative stress. SakA was also identified as HogA (26) and together with AtfA was shown to be involved in gene regulation in response to osmotic stress (27). In many other filamentous fungi, SakA orthologs have been found to be involved in osmotic and/or oxidative-stress resistance and the regulation of development and/or pathogenicity (28)(29)(30)(31)(32).…”

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confidence: 99%

The SrkA Kinase Is Part of the SakA Mitogen-Activated Protein Kinase Interactome and Regulates Stress Responses and Development in Aspergillus nidulans

et al. 2015

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cFungi and many other eukaryotes use specialized mitogen-activated protein kinases (MAPK) of the Hog1/p38 family to transduce environmental stress signals. In Aspergillus nidulans, the MAPK SakA and the transcription factor AtfA are components of a central multiple stress-signaling pathway that also regulates development. Here we characterize SrkA, a putative MAPK-activated protein kinase, as a novel component of this pathway. ⌬srkA and ⌬sakA mutants share a derepressed sexual development phenotype. However, ⌬srkA mutants are not sensitive to oxidative stress, and in fact, srkA inactivation partially suppresses the sensitivity of ⌬sakA mutant conidia to H 2 O 2 , tert-butyl-hydroperoxide (t-BOOH), and menadione. In the absence of stress, SrkA shows physical interaction with nonphosphorylated SakA in the cytosol. We show that H 2 O 2 induces a drastic change in mitochondrial morphology consistent with a fission process and the relocalization of SrkA to nuclei and mitochondria, depending on the presence of SakA. SakA-SrkA nuclear interaction is also observed during normal asexual development in dormant spores. Using SakA and SrkA S-tag pulldown and purification studies coupled to mass spectrometry, we found that SakA interacts with SrkA, the stress MAPK MpkC, the PPT1-type phosphatase AN6892, and other proteins involved in cell cycle regulation, DNA damage response, mRNA stability and protein synthesis, mitochondrial function, and other stress-related responses. We propose that oxidative stress induces DNA damage and mitochondrial fission and that SakA and SrkA mediate cell cycle arrest and regulate mitochondrial function during stress. Our results provide new insights into the mechanisms by which SakA and SrkA regulate the remodelling of cell physiology during oxidative stress and development. W e have proposed that when life was confronted with oxidative stress, cells evolved mechanisms not only to defend against reactive oxygen species (ROS) but also to use this ancestral form of stress to regulate their own growth and differentiation (1, 2). Indeed, the regulated production of ROS by enzymes of the NADPH oxidase family (NOX) is essential for sexual differentiation in Aspergillus nidulans (3) and Neurospora crassa and for polar growth and cell fusion in N. crassa (4), and NOX enzymes play multiple signaling functions in other fungal (5-10), animal, and plant species (11). However, little is known about ROS perception and the mechanisms by which ROS exert their signaling functions.The use of phosphorelay systems to perceive oxidative stress and other types of environmental stress is conserved in bacteria, plants (12), and fungi (13). The fission yeast Schizosaccharomyces pombe uses a multistep phosphorelay composed of the Mak2/3 sensor histidine kinases, Mpr1 HPt protein, and Mcs4 response regulator to transmit H 2 O 2 stress signals to the Spc1 (also known as StyI) mitogen-activated protein kinase (MAPK) cascade (14,15). Spc1 is homologous to Saccharomyces cerevisiae Hog1 and mammalian p38 MAPKs, which are also...

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Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress

Cited by 70 publications

References 76 publications

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

Fungicide: Modes of Action and Possible Impact on Nontarget Microorganisms

The SrkA Kinase Is Part of the SakA Mitogen-Activated Protein Kinase Interactome and Regulates Stress Responses and Development in Aspergillus nidulans

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