Transcriptome analysis and histopathological observations of Geloina erosa gills upon Cr(VI) exposure

Wang, Gongsi; Zhang, Chengkai; Huang, Bo

doi:10.1016/j.cbpc.2020.108706

Cited by 9 publications

(4 citation statements)

References 55 publications

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“…Three mussels from each group were randomly selected for histopathological examination. Two transverse tissue sections and two longitudinal sections were prepared from each individual [36]. Gills were collected, and the samples were fixed using Bouin's solution.…”

Section: Histopathologic Analysismentioning

confidence: 99%

Copper-Induced Ionoregulatory Disturbance, Histopathology, and Transcriptome Responses in Freshwater Mussel (Anodonta woodiana) Gills

Chen

Liu

Liber

et al. 2023

Fishes

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Copper (Cu) contamination has become a severe problem in freshwater environments worldwide. The freshwater mussel Anodonta woodiana is used as a unique bioindicator to monitor Cu contamination in freshwater environments. However, Cu toxicity and response mechanisms in A. woodiana are still largely unknown. A sublethal acute exposure experiment (2.0 mg/L Cu exposure for 72 h) was conducted to investigate the effects of Cu bioaccumulation on ionoregulatory homeostasis, histological features, and transcriptome responses using A. woodiana gills as indicator tissue. The gill bioaccumulation capacity was up to 474. Cu bioaccumulation decreased Na+ and Mg2+ concentrations (p < 0.05) by 82% and 17%, respectively, and induced cilia loss, epithelial desquamation, and filament atrophy of the gills. Transcriptome analysis identified 3160 differentially expressed genes (DEGs), including 1870 upregulated and 1290 downregulated genes. GO enrichment analysis showed that cellular processes, metabolic processes, biological regulation, and responses to stimuli contained the most DEGs in the biological processes. KEGG pathway analysis showed that apoptosis, arginine and proline metabolism, the toll-like receptor signaling pathway, apoptosis-multiple species, histidine metabolism, beta-alanine metabolism, cytokine–cytokine receptor interaction, and the p53 signaling pathway were significantly enriched. These findings provide comprehensive evidence for exploring Cu toxicity and response mechanisms in freshwater mussels.

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Section: Histopathologic Analysismentioning

confidence: 99%

Copper-Induced Ionoregulatory Disturbance, Histopathology, and Transcriptome Responses in Freshwater Mussel (Anodonta woodiana) Gills

Chen

Liu

Liber

et al. 2023

Fishes

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“…Numerous studies have suggested that environmental pollutants, including heavy metals, can activate the TLRs to regulate immune response in animals. For example, Cr(VI) exposure upregulated tlr2 and myd88 expression in Geloina erosa gills [ 78 ], and microbiota-dependent TLR2 signaling reduced silver nanoparticle toxicity to D. rerio larvae [ 79 ]. Relish, an NF-κB transcription factor, also plays a key role in the innate immunity of crustaceans [ 80 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Feng

Song

et al. 2022

Antioxidants

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High concentrations of copper (Cu2+) pose a great threat to aquatic animals. However, the mechanisms underlying the response of crustaceans to Cu2+ exposure have not been well studied. Therefore, we investigated the alterations of physiological and molecular parameters in Chinese mitten crab (Eriocheir sinensis) after Cu2+ exposure. The crabs were exposed to 0 (control), 0.04, 0.18, and 0.70 mg/L of Cu2+ for 5 days, and the hemolymph, hepatopancreas, gills, and muscle were sampled. The results showed that Cu2+ exposure decreased the antioxidative capacity and promoted lipid peroxidation in different tissues. Apoptosis was induced by Cu2+ exposure, and this activation was associated with the mitochondrial and ERK pathways in the hepatopancreas. ER stress-related genes were upregulated in the hepatopancreas but downregulated in the gills at higher doses of Cu2+. Autophagy was considerably influenced by Cu2+ exposure, as evidenced by the upregulation of autophagy-related genes in the hepatopancreas and gills. Cu2+ exposure also caused an immune response in different tissues, especially the hepatopancreas, where the TLR2-MyD88-NF-κB pathway was initiated to mediate the inflammatory response. Overall, our results suggest that Cu2+ exposure induces oxidative stress, ER stress, apoptosis, autophagy, and immune response in E. sinensis, and the toxicity may be implicated following the activation of the ERK, AMPK, and TLR2-MyD88-NF-κB pathways.

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“…G. erosa can use its mantle margin to achieve aerial respiration and its mantle cavity to absorb subterranean water during long-term exposure to the shore ( Morton, 1976 ). They also take up pollutants present in the surrounding environment, which makes them excellent bioindicators of heavy metals and organic pollutants in mangrove environments ( Modassir, 2000 ; Lai et al, 2011 ; Xing et al, 2017 ; Bertucci et al, 2018 ; Ma et al, 2019 ; Almeida et al, 2020 ; Wang et al, 2020 ; Zhang et al, 2020 ). In addition, G. erosa has important economic and medicinal value, Studies have found that the extract of G. erosa has high antiviral activity against influenza virus strains type-A (A/Missisipi 1/85/H3N2) and type-B (B/Harbin 7/94) ( Morton, 1985 ; Chatterji et al, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing Reveals Tissue-Specific Gene Expression Profile of Mangrove Clam Geloina erosa

et al. 2022

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Mollusca is the second largest animal phylum and represents one of the most evolutionarily successful animal groups. Geloina erosa, a species of Corbiculidae, plays an important role in mangrove ecology. It is highly adaptable and can withstand environmental pollution and microbial infections. However, there is no reference genome or full-length transcriptome available for G. erosa. This impedes the study of the biological functions of its different tissues because transcriptome research requires reference genome or full-length transcriptome as a reference to improve accuracy. In this study, we applied a combination of Illumina and PacBio single-molecule real-time sequencing technologies to sequence the full-length transcriptomes of G. erosa tissues. Transcriptomes of nine samples obtained from three tissues (hepatopancreas, gill, and muscle) were sequenced using Illumina. Furthermore, we obtained 87,310 full-length reads non-chimeric sequences. After removing redundancy, 22,749 transcripts were obtained. The average Q score of 30 was 94.48%. In total, 271 alternative splicing events were predicted. There were 14,496 complete regions and 3,870 lncRNAs. Differential expression analysis revealed tissue-specific physiological functions. The gills mainly express functions related to filtration, metabolism, identifying pathogens and activating immunity, and neural activity. The hepatopancreas is the main tissue related to metabolism, it also involved in the immune response. The muscle mainly express functions related to muscle movement and control, it contains more energy metabolites that gill and hepatopancreas. Our research provides an important reference for studying the gene expression of G. erosa under various environmental stresses. Moreover, we present a reliable sequence that will provide an excellent foundation for further research on G. erosa.

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Transcriptome analysis and histopathological observations of Geloina erosa gills upon Cr(VI) exposure

Cited by 9 publications

References 55 publications

Copper-Induced Ionoregulatory Disturbance, Histopathology, and Transcriptome Responses in Freshwater Mussel (Anodonta woodiana) Gills

Copper-Induced Ionoregulatory Disturbance, Histopathology, and Transcriptome Responses in Freshwater Mussel (Anodonta woodiana) Gills

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Full-Length Transcriptome Sequencing Reveals Tissue-Specific Gene Expression Profile of Mangrove Clam Geloina erosa

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