Transcriptome analysis and identification of genes associated with flower development in Rhododendron pulchrum Sweet (Ericaceae)

Wang, Shuzhen; Li, Zhiliang; Jin, Wei-Tao; Fang, Yuanping; Yang, Qiaofeng; Xiang, Jun

doi:10.1016/j.gene.2018.08.083

Cited by 36 publications

(26 citation statements)

References 51 publications

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“…Between stages 3 and 4, DEGs were mainly related to metabolic pathways, antimicrobial and resistance pathways, possibly due to the flowers having already fallen off and the normal development of fruits being more important. These results are similar to those that were found for the development of flowers from other plants [31,32].…”

Section: Gene Regulation During Flower Developmentsupporting

confidence: 91%

Integrative analysis of transcriptome and proteome revealed nectary and nectar traits in the plant-pollinator interaction of Nitraria tangutorum Bobrov

et al. 2021

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Background Nitraria tangutorum is an important desert shrub that shows resistance to drought, salt and wind erosion stresses. It is a central ecological species in its area. Here, we have studied how N. tangutorum has adapted to achieve a successful reproduction strategy. Results We found that N. tangutorum is mainly pollinated by insects of the Hymenoptera, Diptera and Coleoptera orders. Nitraria tangutorum has very small flowers, with the nectary composed of secretive epidermal cells from which nectar is secreted, located within the inner petals. In addition, analyzing the transcriptome of four successive flower developmental stages revealed that mainly differentially expressed genes associated with flower and nectary development, nectar biosynthesis and secretion, flavonoid biosynthesis, plant hormone signal transduction and plant-pathogen interaction show dynamic expression. From the nectar, we could identify seven important proteins, of which the L-ascorbate oxidase protein was first found in plant nectar. Based on the physiological functions of these proteins, we predict that floral nectar proteins of N. tangutorum play an important role in defending against microbial infestation and scavenging active oxygen. Conclusions This study revealed that N. tangutorum is an insect-pollinated plant and its nectary is composed of secretive epidermal cells that specialized into secretive trichomes. We identified a large number of differentially expressed genes controlling flower and nectary development, nectar biosynthesis and secretion, flavonoid biosynthesis, plant hormone signal transduction and plant-pathogen interaction. We suggest that proteins present in N. tangutorum nectar may have both an antibacterial and oxygen scavenging effect. These results provide a scientific basis for exploring how the reproductive system of N. tangutorum and other arid-desert plants functions.

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Section: Gene Regulation During Flower Developmentsupporting

confidence: 91%

Integrative analysis of transcriptome and proteome revealed nectary and nectar traits in the plant-pollinator interaction of Nitraria tangutorum Bobrov

et al. 2021

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“…CYCD3 overexpression caused delayed morphological differences in SAM and leaf senescence [14]. Additionally, CYCD3 was also found to be expressed at its highest levels during the flower bud stage in Rhododendron [10]. Taihangia no Cyclin_C domain; thus, the Cyclin_C domain may not be critical for its function [28].…”

Section: Discussionmentioning

confidence: 99%

“…Six homologous genes, including knotted1-like homeobox gene 6 (KNAT6) and MYC2, may be candidate genes for sex differentiation [9]. During the flower bud stage in Rhododendron, transcripts of MYC2, TIR1, CYCD3, COL-1, and EIN3 peak [10]. Additionally, upregulation of CYCD3 is thought to be associated with increased inflorescence meristems of J. curcas [11].…”

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confidence: 99%

Cloning and functional analysis of JnCYCD3;1 in Jatropha nigroviensrugosus

Feng

Yang²,

Wang

2020

Preprint

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Background: As a new variety of Jatropha, the female to male flower ratio and yield of Jatropha nigroviensrugosus are higher than the common J. curcas. Using pre-transcriptome data, the full-length gene sequence, subcellular function localization, and verification of the transgenic function were obtained in order to understand the specific functions of differentially expressed genes (DEGs). Results: Results revealed that the open reading frame (ORF) of J. nigroviensrugosus CYCD3;1 (JnCYCD3;1) was 414 bp long, encoding 137 amino acids (aa). Compared to J. curcas, the presence of intron retention led to early termination of the coding frame. JnCYCD3;1 had the highest expression in new leaves, which was 68.42 times root expression, followed by inflorescence buds. The JnMYC2 ORF was 2025 bp, encoding 674 aa. JnMYC2 had the highest expression levels in inflorescence buds. JnCYCD3;1 functioned in the nucleus, while JnMYC2 was distributed in both the nucleus and cytoplasm and may possess transmembrane membrane behavior. Bimolecular fluorescence complementation (BIFC) experiments indicated that JnMYC2 interacted with JnCYCD3;1. JnCYCD3;1 transgenic tobacco considerably advanced the reproductive cycle and may promote flower formation and transformation. Conclusions:The related experiments obtained new CYCD3;1 transcript, verified that CYCD3;1 is related to flower bud differentiation, proved the interaction between hormones-related genes. The study provides a new research direction for gene function of CYCD3;1.

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“…Pelargonidin, cyanidin, quercetin, catechin, gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, and p-coumaric acid was detected in flower extract of rose 'KORcrisett' (Schmitzer et al, 2009). Schmitzer et al (2010) (Wang et al, 2018),carbohydrates level (Yamada et al, 2007), change in cell membrane stability (Faragher et al, 1987), food remobilization, and total protein (Kumar et al, 2008). Efforts have been made to increase polyphenols quantity in order to use in the human diet (Mrad et al, 2012;Chamorro et al, 2012;Vallverdú-Queralt et al, 2013;Do et al, 2014), therefore selecting the best stage of flowering for polyphenol extraction is very important as a strategy for obtaining the highest polyphenols content.…”

Section: Introductionmentioning

confidence: 99%

Phenolics pattern of cut H3O rose flowers during floral development

Chamani

Wagstaff

Kanani

2020

Scientia Horticulturae

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An experiment was conducted to evaluate the phenolic acids and flavonoids contents from flower bud to flower senescence stages (7 stages) in cut H3O roses every other day. Some phenolic acids (Gallic, Caffeic, Caftaric, Chlorogenic, Chiconic, Coumaric, Sinapic, and Ferulic) and flavonoids (Catechin, Pelargonidin chloride, Cyanidin, Kaempferol, and Quercetin) were detected in the methanolic extract of rose petals by HPLC. Data analysis revealed that Gallic acid content did not significantly (P≤0.05) affects from the flower bud to the senescence stages. However, it's found that Caftaric acid, Chlorogenic acid, Coumaric acid, Ferulic acid, Chiconic acid, Quercetin, and Kaempferol (at P≤0.05), Catechin, Caffeic acid, Sinapic acid, Cyanidin, and Pelargonidin chloride contents (at P≤0.01) significantly affected during flower opening stages. The highest contents of phenolics during development of cut H3O rose flowers were as follow: day 1 [Coumaric acid], day 3 [Caftaric acid, Ferulic acid, and Kaempferol], day 5 [no compound was found], day 7 [Sinapic acid and Cyanidin], day 9 [Chlorogenic acid], day 11 [Chiconic acid and Quercetin], and day 13 [Caffeic acid, Catechin, and Pelargonidin chloride]. The results showed that flower development stages can be suggested to consider the phenolic compounds required to use in the therapeutic process.

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Transcriptome analysis and identification of genes associated with flower development in Rhododendron pulchrum Sweet (Ericaceae)

Cited by 36 publications

References 51 publications

Integrative analysis of transcriptome and proteome revealed nectary and nectar traits in the plant-pollinator interaction of Nitraria tangutorum Bobrov

Integrative analysis of transcriptome and proteome revealed nectary and nectar traits in the plant-pollinator interaction of Nitraria tangutorum Bobrov

Cloning and functional analysis of JnCYCD3;1 in Jatropha nigroviensrugosus

Phenolics pattern of cut H3O rose flowers during floral development

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