Transcriptome Analysis and Identification of Chemosensory Genes in Baryscapus dioryctriae (Hymenoptera: Eulophidae)

Abstract

Baryscapus dioryctriae is a pupal endoparasitoid of many Pyralidae pests and has been used as a biocontrol agent against insect pests that heavily damage the cone and seed of the Korean pine. The olfactory system of wasps plays an essential role in sensing the chemical signals during their foraging, mating, host location, etc., and the chemosensory genes are involved in detecting and transducing these signals. Many chemosensory genes have been identified from the antennae of Hymenoptera; however, there are few reports on the chemosensory genes of Eulophidae wasps. In this study, the transcriptome databases based on ten different tissues of B. dioryctriae were first constructed, and 274 putative chemosensory genes, consisting of 27 OBPs, 9 CSPs, 3 NPC2s, 155 ORs, 49 GRs, 23 IRs and 8 SNMPs genes, were identified based on the transcriptomes and manual annotation. Phylogenetic trees of the chemosensory genes were constructed to investigate the orthologs between B. dioryctriae and other insect species. Additionally, twenty-eight chemosensory genes showed female antennae- and ovipositor-biased expression, which was validated by RT-qPCR. These findings not only built a molecular basis for further research on the processes of chemosensory perception in B. dioryctriae, but also enriched the identification of chemosensory genes from various tissues of Eulophidae wasps.

Transcriptome analysis and identification of genes related to environmental adaptation of Grylloprimevala jilina Zhou & Ren 2023

“…All the used amino acid sequences in this study are presented in the Appendix S1. Using the “One Step Build a ML Tree” function of TBtools (Zhu et al., 2022), the Muscle program was used for multiple sequence alignment, TrimAl was used to prune the alignment results, and finally, IQ‐tree was used to automatically screen the amino acid replacement model to build the maximum likelihood (ML) trees (Chen et al., 2020), which were visualized with FigTree v1.4. (Drummond et al., 2012).…”

Functional Characterization of the Niemann–Pick C2 Protein BdioNPC2b in the Parasitic Wasp Baryscapus dioryctriae (Chalcidodea: Eulophidae)

“…To identify the antennal BdioNPC 2s, we reassembled the female and male antennal transcripts of B. dioryctriae (SRR21665109 and SRR21665108, respectively) based on our previous study. 23 The putative BdioNPC2 genes were screened from the reassembled antennal-transcriptome annotation results. The ORF Finder tool (https://www.ncbi.nlm.nih.gov/orffinder/, accessed on 25 November 2023) was used to predict the open reading frame (ORF) and amino acid sequence of each BdioNPC2 gene.…”

“…The parasitic wasp Baryscapus dioryctriae (Hymenoptera: Chalcidodea: Eulophidae) was originally identified as a gregarious pupal endoparasitoid wasp of Dioryctria (Lepidoptera: Pyralidae) pests, such as the coneworms D. abietella, D. pryeri, and D. rubella. 22,23 Dioryctria pests damage the cones, seeds, and twigs of Pinus trees and severely damage the forestry economy and environment. To apply B. dioryctriae for the biocontrol of these Pinus pests, B. dioryctriae was reared in mass culture media.…”

“…However, B. dioryctriae cannot localize to these alternative hosts in the field, which has restricted the wide application of this wasp in pest biocontrol. 22,23 It would be helpful to address the challenges associated with the wide application of B. dioryctriae by studying the olfactory mechanism underlying its chemical communication during adulthood. We investigated the ultrastructure of the antennal olfactory sensilla of this wasp 22 and identified olfactory genes, including 27 OBPs, 9 CSPs, 8 SNMPs, 155 ORs, 23 IRs, 49 GRs (gustatory receptors), and 3 NPC 2s, from its 10 tissue transcriptomes.…”

“…We investigated the ultrastructure of the antennal olfactory sensilla of this wasp 22 and identified olfactory genes, including 27 OBPs, 9 CSPs, 8 SNMPs, 155 ORs, 23 IRs, 49 GRs (gustatory receptors), and 3 NPC 2s, from its 10 tissue transcriptomes. 23 To study these chemosensory gene families, starting from a functional study on NPC 2s might be an appropriate approach for exploring the olfactory molecular mechanism of B. dioryctriae because of the smaller number of NPC 2s than that of olfactory genes from the other families. Three BdioNPC 2s were identified in our previous study, yet none of the three BdioNPC 2s exhibited antenna-biased expression.…”