Transcriptome Analysis and Identification of Major Detoxification Gene Families and Insecticide Targets in Grapholita Molesta (Busck) (Lepidoptera: Tortricidae)

Guo, Yingjie; Chai, Yanping; Zhang, Lijun; Zhao, Zhiguo; Gao, Lingling; Ma, Ruiyan

doi:10.1093/jisesa/iex014

Cited by 21 publications

(23 citation statements)

References 84 publications

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“…For molecular functions, the most common GO terms were “catalytic activity” (1776 unigenes) and “binding” (1475 unigenes). The unigene distribution patterns among the three GO categories were similar to those observed for Grapholita molesta [ 26 ] and Shirakiacris shirakii [ 24 ]. Furthermore, a number ofunigenes were classified to some GO categoriesthat may contribute to tissue and organ morphogenesis in X. brachycerus , such as “structural constituent of chitin-based cuticle”, “chitin binding”, “mesoderm development”, “chitin metabolic process”, “tissue development”, and “structural constituent of the cuticle”.…”

Section: Resultssupporting

confidence: 69%

De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome

Zhao

Zhang

Qiu

et al. 2018

IJMS

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Grasshoppers are common pests but also have high nutritional and commercial potential. Xenocatantops brachycerus Willemse (Orthoptera: Acrididae) is an economically important grasshopper species that is reared in China. Using the IlluminaHiSeqTM 4000 platform, three transcriptomes of the adult male, adult female, and nymph of X. brachycerus were sequenced. A total of 133,194,848 clean reads were obtained and de novo assembled into 43,187 unigenes with an average length of 964 bp (N50 of 1799 bp); of these, 24,717 (57.23%) unigenes matched known proteins. Based on these annotations, many putative transcripts related to X. brachycerus growth, development, environmental adaptability, and metabolism of nutritional components and bioactive components were identified. In addition, the expression profiles of all three transcriptome datasets were analyzed, and many differentially expressed genes were detected using RSEM and PossionDis. Unigenes. Unigenes with functions associated with growth and development exhibited higher transcript levels at the nymph stage, and unigenes associated with environmental adaptability showed increased transcription in the adults. These comprehensive X. brachycerus transcriptomic data will provide a useful molecular resource for gene prediction, molecular marker development, and studies on signaling pathways in this species and will serve as a reference for the efficient use of other grasshoppers.

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Section: Resultssupporting

confidence: 69%

De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome

Zhao

Zhang

Qiu

et al. 2018

IJMS

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“…The GST superfamily has been identified in many insect species. For instance, 41, 27, 18, 28, and 36 GSTs have been identified in Drosophila melanogaster , Bactrocera dorsalis , Bactrocera minax , Grapholita molesta , and Shirakiacris shirakii , respectively [ 38 , 61 , 35 , 66 ]. We identified 21 GST genes in P .…”

Section: Discussionmentioning

confidence: 99%

“…shirakii , Anopheles sinensis , Grapholita molesta , B . dorsalis , and Bombyx mori , respectively [ 66 , 71 , 35 , 38 , 93 ]. In this study, 17 CarEs were identified in the transcriptome of the P .…”

Section: Discussionmentioning

confidence: 99%

“…Likewise, GSTs more widely detoxify insecticides and plant allelochemicals in insects and are a superfamily of enzymes widespread in most organisms [ 33 – 34 ]. GSTs can be classified into seven classes in insects, including the delta, epsilon, omega, sigma, theta, zeta, and microsomal groups, where the delta and epsilon classes are insect-specific [ 35 – 37 ]. Lastly, CarEs belong to a superfamily of metabolic enzymes that are generally distributed throughout the various parts of insects and play significant roles in the metabolic detoxification of xenobiotics, the degradation of hormones, in neurodevelopment, and in defense [ 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

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De novo transcriptomic analysis of the alimentary tract of the tephritid gall fly, Procecidochares utilis

Lan

et al. 2018

PLoS ONE

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The tephritid gall fly, Procecidochares utilis, is an important obligate parasitic insect of the malignant weed Eupatorium adenophorum which biosynthesizes toxic secondary metabolites. Insect alimentary tracts secrete several enzymes that are used for detoxification, including cytochrome P450s, glutathione S-transferases, and carboxylesterases. To explore the adaptation of P. utilis to its toxic host plant, E. adenophorum at molecular level, we sequenced the transcriptome of the alimentary tract of P. utilis using Illumina sequencing. Sequencing and de novo assembly yielded 62,443 high-quality contigs with an average length of 604 bp that were further assembled into 45,985 unigenes with an average length of 674 bp and an N50 of 983 bp. Among the unigenes, 30,430 (66.17%) were annotated by alignment against the NCBI non-redundant protein (Nr) database, while 16,700 (36.32%), 16,267 (35.37%), and 11,530 (25.07%) were assigned functions using the Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Ontology (GO) databases, respectively. Using the comprehensive transcriptome data set, we manually identified several important gene families likely to be involved in the detoxification of toxic compounds including 21 unigenes within the glutathione S-transferase (GST) family, 22 unigenes within the cytochrome P450 (P450) family, and 16 unigenes within the carboxylesterase (CarE) family. Quantitative PCR was used to verify eight, six, and two genes of GSTs, P450s, and CarEs, respectively, in different P. utilis tissues and at different developmental stages. The detoxification enzyme genes were mainly expressed in the foregut and midgut. Moreover, the unigenes were higher expressed in the larvae, pupae, and 3-day adults, while they were expressed at lower levels in eggs. These transcriptomic data provide a valuable molecular resource for better understanding the function of the P. utilis alimentary canal. These identified genes could be pinpoints to address the molecular mechanisms of P. utilis interacting with toxic plant host.

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“…The control of PFM in plum orchards has been mainly based on the use of chemical insecticides, which can cause side effects on beneficial insects, both pollinators and natural enemies, and can have serious implications for human health and the environment [ 14 ], or the development of resistance, as found in other tortricids, like Grapholita molesta Busck [ 15 , 16 ], Cydia pomonella L. [ 17 , 18 , 19 ] and Lobesia botrana (Denis & Schiffermuller) [ 20 ]. Moreover, in organic plum orchards, moth control is particularly difficult due to the small number of authorised products.…”

Section: Introductionmentioning

confidence: 99%

Can Mating Disruption Be a Possible Route to Control Plum Fruit Moth in Mediterranean Environments?

Verde

Guarino

Barone

et al. 2020

Insects

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Control of the plum fruit moth, Grapholita funebrana Treitschke (Lepidoptera: Tortricidae), has been mainly based on the use of chemical insecticides, which can cause undesirable side effects, leading to a growing interest towards alternative sustainable strategies. The aim of this study was to evaluate the effect of the mating disruption technique on G. funebrana infestation in plum orchards, by comparing the number of male captures in pheromone-baited traps, and evaluating the damage to fruits in plots treated with the pheromone dispersers and in control plots. The study was carried out in 2012 and 2014 in three organic plum orchards, on the cultivars Angeleno, Friar, President and Stanley. To evaluate the pheromone emission curve of the dispensers from the openings to the end of the trials, a chemical analysis was carried out by solid phase micro-extraction followed by gas chromatography, followed by mass spectrometry. In all years and orchards the mean number of males caught in traps placed in the treatment plots was always significantly lower than untreated plots. Pheromone emission from the dispensers was highest at the opening, and was still considerable at 54 days of field exposure, while it significantly decreased after 72 days of field exposure. Cultivar was confirmed to be an essential factor in determining the fruit infestation level. Pheromone treatment significantly reduced fruit infestation, but not economic damage.

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Transcriptome Analysis and Identification of Major Detoxification Gene Families and Insecticide Targets in Grapholita Molesta (Busck) (Lepidoptera: Tortricidae)

Cited by 21 publications

References 84 publications

De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome

De Novo Assembly and Characterization of the Xenocatantops brachycerus Transcriptome

De novo transcriptomic analysis of the alimentary tract of the tephritid gall fly, Procecidochares utilis

Can Mating Disruption Be a Possible Route to Control Plum Fruit Moth in Mediterranean Environments?

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