Transcriptome analysis and identification of chemosensory genes in the larvae of Plagiodera versicolora

Abstract

Background In insects, the chemosensory system is crucial in guiding their behaviors for survival. Plagiodera versicolora (Coleoptera: Chrysomelidae), is a worldwide leaf-eating forest pest in salicaceous trees. There is little known about the chemosensory genes in P. versicolora. Here, we conducted a transcriptome analysis of larvae heads in P. versicolora. Results In this study, 29 odorant binding proteins (OBPs), 6 chemosensory proteins (CSPs), 14 odorant receptors (ORs), 13 gustatory receptors (GRs), 8 ionotropic receptors (IRs) and 4 sensory neuron membrane proteins (SNMPs) were identified by transcriptome analysis. Compared to the previous antennae and foreleg transcriptome data in adults, 12 OBPs, 2 CSPs, 5 ORs, 4 IRs, and 7 GRs were newly identified in the larvae. Phylogenetic analyses were conducted and found a new candidate CO2 receptor (PverGR18) and a new sugar receptor (PverGR23) in the tree of GRs. Subsequently, the dynamic expression profiles of various genes were analyzed by quantitative real-time PCR. The results showed that PverOBP31, OBP34, OBP35, OBP38, and OBP40 were highly expressed in larvae, PverOBP33 and OBP37 were highly expressed in pupae, and PverCSP13 was highly expressed in eggs, respectively. Conclusions We identified a total of 74 putative chemosensory genes based on a transcriptome analysis of larvae heads in P. versicolora. This work provides new information for functional studies on the chemoreception mechanism in P. versicolora.

Odorant binding protein 18 increases the pathogen resistance of the imported willow leaf beetle, Plagiodera versicolora

“…In previous study, the chemosensory genes of P. versicolora had been identified based on transcriptome of larvae, adult antennas and forelegs, respectively. Collectively, a total of 40 candidate OBPs of P. versicolora had been identified ( Liu et al., 2021 ; Wu et al., 2022a ; Wu et al., 2022b ).…”

Relish involved in immunity and larval survival in the willow leaf beetle Plagiodera versicolora

“…26 The willow leaf beetle, Plagiodera versicolora (Laicharting; Coleoptera: Chrysomelidae), is a specialist herbivore of salicaceous trees. 27,28 There is a gap in research on the identification and functional analysis of the IMD pathway in P. versicolora. This work aimed to explore interactions within the IMD pathway, specifically focusing on the Relish gene.…”

Correction: Transcriptome analysis and identification of chemosensory genes in the larvae of Plagiodera versicolora

“…Following the publication of the original article [ 1 ], it was noted that the figure legends were paired incorrectly. The figure legends for Figs.…”