Transcriptome analysis of boar spermatozoa with different freezability using RNA-Seq

Fraser, Leyland; Brym, Paweł; Pareek, Chandra Shekhar; Mogielnicka-Brzozowska, Marzena; Paukszto, Łukasz; Jastrzebski, J; Wasilewska-Sakowska, Karolina; Mańkowska, Anna; Sobiech, Przemysław; Żukowski, Kacper

doi:10.1016/j.theriogenology.2019.11.001

Cited by 63 publications

(63 citation statements)

References 69 publications

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“…Our recent study has confirmed that there are differences in the transcriptome profiles of boar spermatozoa [8]. Furthermore, it has been confirmed that boars with poor freezability ejaculates are characterized by an overexpression of differentially expressed genes (DEGs) that are mainly associated with inflammation and apoptosis, which increase the sperm susceptibility to cryo-induced damage [8]. Previous studies confirmed that genes enriched in the cytokine-cytokine receptor interaction and inflammatory response-related pathways predisposed boar sperm to cryo-induced damage [9,10].…”

Section: Introductionsupporting

confidence: 62%

“…Recent developments in high-throughput sequencing techniques, such as transcriptome sequencing (RNA-Seq), have enabled a thorough analysis of gene expression and genetic variations in the pig reproductive tract [7]. Our recent study has confirmed that there are differences in the transcriptome profiles of boar spermatozoa [8]. Furthermore, it has been confirmed that boars with poor freezability ejaculates are characterized by an overexpression of differentially expressed genes (DEGs) that are mainly associated with inflammation and apoptosis, which increase the sperm susceptibility to cryo-induced damage [8].…”

Section: Introductionsupporting

confidence: 56%

“…Previous studies confirmed that genes enriched in the cytokine-cytokine receptor interaction and inflammatory response-related pathways predisposed boar sperm to cryo-induced damage [9,10]. Hence, transcriptome alterations in spermatozoa have been considered to be one of the main factors affecting the cryo-survival of boar semen [8][9][10]. It should be emphasized that variant calling procedure based on RNA-Seq data has been suggested to be an important screening tool to identify polymorphisms in differentially expressed genes [7] and potential genetic markers associated with production traits in the pig industry [11].…”

Section: Introductionmentioning

confidence: 88%

“…A total of forty PLW boars were used in this study. The six boars used for RNA-Seq were stationed at the Cryopreservation laboratory, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn [8]. For the association studies, most of the ejaculates were collected from boars, which were stationed at the Cryopreservation laboratory, and a total of 51 ejaculates were collected from boars stationed at three AI centers [5].…”

Section: Animals and Semen Collectionsmentioning

confidence: 99%

“…We used the RNA-Seq datasets that were submitted to the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database (Bioproject: PRJNA415904; accession number SRP121647) (2018/05/27). The datasets represent RNA-Seq from three PLW boars each of the GSF and PSF groups [8].…”

Section: Variant Calling and Filtering Analysismentioning

confidence: 99%

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Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Mańkowska

Brym

Paukszto

et al. 2020

IJMS

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Genetic markers have been used to assess the freezability of semen. With the advancement in molecular genetic techniques, it is possible to assess the relationships between sperm functions and gene polymorphisms. In this study, variant calling analysis of RNA-Seq datasets was used to identify single nucleotide polymorphisms (SNPs) in boar spermatozoa and to explore the associations between SNPs and post-thaw semen quality. Assessment of post-thaw sperm quality characteristics showed that 21 boars were considered as having good semen freezability (GSF), while 19 boars were classified as having poor semen freezability (PSF). Variant calling demonstrated that most of the polymorphisms (67%) detected in boar spermatozoa were at the 3’-untranslated regions (3’-UTRs). Analysis of SNP abundance in various functional gene categories showed that gene ontology (GO) terms were related to response to stress, motility, metabolism, reproduction, and embryo development. Genomic DNA was isolated from sperm samples of 40 boars. Forty SNPs were selected and genotyped, and several SNPs were significantly associated with motility and membrane integrity of frozen-thawed (FT) spermatozoa. Polymorphism in SCLT1 gene was associated with significantly higher motility and plasma membrane integrity of FT spermatozoa from boars of the GSF group compared with those of the PSF group. Likewise, polymorphisms in MAP3K20, MS4A2, and ROBO1 genes were significantly associated with reduced cryo-induced lipid peroxidation and DNA damage of FT spermatozoa from boars of the GSF group. Candidate genes with significant SNP associations, including APPL1, PLBD1, FBXO16, EML5, RAB3C, OXSR1, PRICKLE1, and MAP3K20 genes, represent potential markers for post-thaw semen quality, and they might be relevant for future improvement in the selection procedure of boars for cryopreservation. The findings of this study provide evidence indicating that polymorphisms in genes expressed in spermatozoa could be considered as factors associated with post-thaw semen quality.

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Section: Introductionsupporting

confidence: 62%

Section: Introductionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 88%

Section: Animals and Semen Collectionsmentioning

confidence: 99%

Section: Variant Calling and Filtering Analysismentioning

confidence: 99%

See 3 more Smart Citations

Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Mańkowska

Brym

Paukszto

et al. 2020

IJMS

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Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep

Zhang

Guo

et al. 2023

Molecular Reproduction Devel

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Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) regulates mammalian ovarian follicle growth and maturation; however, its effect on luteinized granulosa cells (LGCs) in sheep ovarian follicles remains unknown.Here we explored the regulatory role of LGC functions and steroid hormone synthesis by BAMBI. Multiple sequence alignment revealed that the sheep BAMBI gene sequence was relatively conserved. Sheep LGCs were strongly positive for BAMBI. LGC proliferation increased when BAMBI was silenced and decreased when BAMBI was overexpressed. After BAMBI overexpression, the expression of CASP3, CASP8, CASP9, and BAX significantly increased, whereas that of BCL2 and the ratio of BCL2/BAX expression decreased. The opposite was observed after BAMBI silencing. CDKN1A, CCND1, and CCND2 were downregulated with BAMBI overexpression and upregulated with BAMBI silencing. Expression of steroid hormonerelated genes (CYP11A1, STAR, and 3BHSD), except CYP19A1, significantly increased after BAMBI overexpression. Moreover, estrogen and progesterone secretion increased after BAMBI overexpression and decreased after BAMBI interference.The effect of the exogenous addition of bone morphogenetic protein 2 (BMP2) on GCs was similar to that of BAMBI overexpression. In conclusion, BAMBI can regulate the proliferation and steroid hormone synthesis of sheep LGCs, and BMP2 can affectLGCs as an activator of BAMBI. These findings provide a basis for further research on the physiological role of BAMBI.

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Genome-Wide Association Study (GWAS) for Trait Analysis in Crops

Kumari

Muduli

Meher

et al. 2022

Springer Protocols Handbooks

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Transcriptome analysis of boar spermatozoa with different freezability using RNA-Seq

Cited by 63 publications

References 69 publications

Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality

Effects of BAMBI on luteinized follicular granulosa cell proliferation and steroid hormone production in sheep

Genome-Wide Association Study (GWAS) for Trait Analysis in Crops

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