Transcriptome analysis of bolting in A. tequilana reveals roles for florigen, MADS, fructans and gibberellins

Dios, Emmanuel Ávila de; Delaye, Luis; Simpson, June

doi:10.1186/s12864-019-5808-9

Cited by 20 publications

(39 citation statements)

References 45 publications

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“…Transcriptome analysis of the vegetative to reproductive transition in A. tequilana revealed no differential expression for starch metabolism related genes (Zavala- Garcia et al, 2018) whereas fructan related genes are highly expressed in SAM tissue in comparison to leaf tissue. In particular a 6G-FFT isoform is specifically and strongly expressed at the initial stage of the reproductive phase (Avila De Dios et al, 2019).…”

Section: Agave Fructansmentioning

confidence: 99%

“…Delgado Sandoval et al, 2012, showed that as the reproductive stage initiates, development of photosynthetically active leaves is suppressed and the SAM differentiates. Genes encoding FEH and invertases increase their expression during bolting (Avila De Dios et al, 2019) and leaves and stems senesce indicating that carbohydrate reserves are being harnessed for flowering.…”

Section: Biological Functions Of Agave Fructansmentioning

confidence: 99%

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The Sweet Taste of Adapting to the Desert: Fructan Metabolism in Agave Species

Pérez-López

Simpson

2020

Front. Plant Sci.

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Over 70% of Agave species, (159 of 206) are found in Mexico and are well adapted to survive under hot, arid conditions, often in marginal terrain, due to a unique combination of morphological and physiological attributes. In the pre-Columbian era agaves were also key to human adaptation to desert terrain. In contrast to other species such as cacti or resurrection plants, Agaves store carbohydrates in the form of fructan polymers rather than starch or sucrose, however, properties specific to fructans such as a strong hydration shell, the ability to be transported through phloem, variable composition throughout the Agave life-cycle and accumulation in succulent tissues and flowers suggest a potential for multiple functional roles. This mini-review summarizes current knowledge of molecular and biochemical aspects of fructan metabolism in Agave species.

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Section: Agave Fructansmentioning

confidence: 99%

Section: Biological Functions Of Agave Fructansmentioning

confidence: 99%

The Sweet Taste of Adapting to the Desert: Fructan Metabolism in Agave Species

Pérez-López

Simpson

2020

Front. Plant Sci.

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“…16,73 At present, there is scarce information on the genomic organization of the Agave genus, and also a few transcriptome and proteomic analyses of Agave species, which makes it difficult to identify putative protein-coding genes that respond to drought, heat stress, and flowering. [73][74][75] It is hoped that future studies will identify essential genetic and biochemical mechanisms beneficial to biotechnology geared toward engineering drought-and disease-tolerant C 3 and C 4 plants, and even interspecies agaves of interest. 76 Nevertheless, in silico analyses have already identified potential cellulase synthases, glucanases, and sucrose synthases that could be utilized in fiber production.…”

Section: Agave Drought Tolerance and Synthetic Biologymentioning

confidence: 99%

Agave: a natural renewable resource with multiple applications

et al. 2020

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Agaves are a group of succulent plants that thrive in arid or semiarid environments. Indeed, genes associated with their resilience are a potential resource for genetic engineering of other agronomically important crops grown in adverse climates. Agave is mainly used for the production of distilled (spirits) and non-distilled alcoholic beverages, including tequila, mezcal, bacanora, raicilla, and pulque, all of which have special connections to Mexican history and culture, and contribute to the Mexican economy. In recent years, there has been growing interest to maximize the use of agave plant materials for other purposes, as the bulk of their biomass pre-and post-production is wasted. In traditional practice, during the passage from fields to factories, only agave cores are used, and the leaves and bagasse are not always harnessed. To place this in perspective, during the period from 2010 to 2019, 2674.7 × 10 6 L of tequila was produced in Mexico, which required 9 607 400 tons of agave cores. This generated approximately the same amount of leaves and 3 842 960 tons of bagasse. The economic base of agave plants can be expanded if expended biomass could be transformed into products that are useful for applications in food, forage, ensilage, agriculture, medicine, energy, environment, textiles, cosmetics, and esthetics. This review focuses on the current utility of agave plants, as well as our perspective for future studies and uses of this formidable plant.

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“…Two-year RNA-seq analysis in different tissue -For the estimation of tissue-specific expression, we consulted the assembled transcriptomic library, obtained by Illumina sequencing, in different tissues and the stages of development of A. tequilana, by Ávila de Dios at the Simpson CIN-VESTAV-Irapuato Laboratory. Extraction, preparation, and TSA library assembly are described in detail by (De Dios et al 2019).…”

Section: Analysis Of the Amino Acid And Structural Sequence Of Thementioning

confidence: 99%

AteqTERT expression and specific tissue activity in a 2-year-old complete plant in Agave tequilana in field conditions

et al. 2020

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Telomerase is a specialized enzyme that attributes to maintaining and lengthening of telomeric length as its primary function. This ribonucleoprotein complex requires two minimum subunits for its in vivo function: the TERT amino acid subunit and TER its ribonucleic subunit. Crystallographic studies have shown that stable interaction between subunits and their domains is essential during the active phase. Plants and humans have shown to be tissue specific and dependent on cell proliferation. In Arabidopsis thaliana (L.) Heynh, the expression of AtTERT was determined to be necessary for activity. The objective of this work is to perform the in silico characterization of TERT in Agave tequilana F.A.C. Weber and evaluate the expression of TERT and the specific tissue activity in 2-year-old plants. To this end, we use the transcriptome library of A. tequilana (Ávila de Dios unpublished data) in TPM and qTRAP. We observe in this work that the consensus sequence shows all the specific domains of the TERT subunit. Additionally, we carried out the in silico prediction of the structural model of AteqTERT. The model presents the characteristic TERT ring of the crystallized models in active state of telomerase, biological annotations were observed, such as heavy metals Mg 2+ , NUC and CH1, and finally we note that, there is no correlation between TERT expression in TPM and telomerase activity in 2-year meristematic tissue.

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Transcriptome analysis of bolting in A. tequilana reveals roles for florigen, MADS, fructans and gibberellins

Cited by 20 publications

References 45 publications

The Sweet Taste of Adapting to the Desert: Fructan Metabolism in Agave Species

The Sweet Taste of Adapting to the Desert: Fructan Metabolism in Agave Species

Agave: a natural renewable resource with multiple applications

AteqTERT expression and specific tissue activity in a 2-year-old complete plant in Agave tequilana in field conditions

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