Transcriptome analysis of callus from Picea balfouriana

Li, Qingfen; Zhang, Shougong; Wang, Junhui

doi:10.1186/1471-2164-15-553

Cited by 40 publications

(32 citation statements)

References 56 publications

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“…Temperature should be an important factor controlling tree growth rates. Similar results were found on Western Sichuan Plateau (Duan et al, 2010;Li et al, 2014). Since tree growth was not limited by water condition, higher mean summer temperature could contribute to tree growth by stimulating photosynthesis.…”

Section: The Climatic Information Recorded In the Tree-ring Width Andsupporting

confidence: 82%

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Long-term changes in the tree radial growth and intrinsic water-use efficiency of Chuanxi spruce (Picea likiangensis var. balfouriana) in southwestern China

Wang¹,

Zhang²,

Fang³

et al. 2018

J. Geogr. Sci.

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Elevated CO 2 level in the atmosphere is expected to improve the tree growth rates and intrinsic water-use efficiency (iWUE). Although current results inferring from tree rings found the tree growth decline in water-limited area, it is still unclear whether spruce trees in humid southwestern China benefit from the increasing CO 2. In this study, tree-ring width data were used to investigate the tree radial growth rate of Chuanxi spruce (Picea likiangensis var. balfouriana). Moreover, combining with the tree-ring carbon isotope date, we analyzed the physiological responses of Chuanxi spruce to rising CO 2 concentrations in the atmosphere (C a) associated with climatic change in southwestern China. From 1851 to 2009, iWUE of Chuanxi spruce rose by approximately 30.4%, and the ratio of atmospheric CO 2 to leaf intercellular CO 2 concentration (C i /C a) showed no significant trend in the study area. The result suggested that Chuanxi spruce used an active response strategy when C a was significantly increased. iWUE showed a significant increasing trend in parallel with tree radial growth, indicating that the increasing iWUE resulted in an increase in radial growth. These results suggest that spruce forests in southwestern China have not shown declining trends under increasing C a and climate change scenarios, in contrast to trees growing in water-limited areas. Therefore, spruce forests benefit from the increasing CO 2 in the atmosphere in the humid areas of southwestern China.

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Section: The Climatic Information Recorded In the Tree-ring Width Andsupporting

confidence: 82%

“…In southwestern China, Chuanxi spruce (Picea balfouriana) plays a key role in environmental protection and wood production (Li et al, 2014). It is crucial to evaluate how spruce trees respond to C a increases and to climate change.…”

Section: Introductionmentioning

confidence: 99%

Long-term changes in the tree radial growth and intrinsic water-use efficiency of Chuanxi spruce (Picea likiangensis var. balfouriana) in southwestern China

Wang¹,

Zhang²,

Fang³

et al. 2018

J. Geogr. Sci.

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“…Information regarding the expression of AGP genes in in vitro embryogenesis is very scarce. Some studies have shown up-regulation of some AGP genes associated with the initiation of somatic embryogenesis in Picea balfouriana (Li et al, 2014b) and microspore embryogenesis in B. napus (Malik et al, 2007;El-Tantawy et al, 2013).…”

Section: Cell Wall Remodelling: Pectins and Agpsmentioning

confidence: 99%

Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement

Testillano¹

2019

Journal of Experimental Botany

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Under stress, isolated microspores are reprogrammed in vitro towards embryogenesis, producing doubled haploid plants, useful biotechnological tools in plant breeding as a source of new genetic variability, fixed in homozygous plants in only one generation. Stress-induced cell death and low rates of cell reprogramming are major factors that reduce the process yield. Knowledge gained in recent years has revealed that microspore embryogenesis initiation and progression involve a complex network of factors, whose roles are not yet well understood. Autophagy and cell-death proteases are crucial players in the response to stress, while cell reprogramming and totipotency acquisition are regulated by hormonal and epigenetic mechanisms. Auxin biosynthesis, transport and action are required for microspore embryogenesis. Initial stages involve DNA hypomethylation, H3K9 demethylation, and H3/H4 acetylation. Cell wall remodelling, with pectin de-methylesterification and AGP expression, is necessary for embryo development. We will review recent findings regarding the determinant factors underlying stress-induced microspore embryogenesis, focusing on the role of autophagy, cell death, auxin, chromatin modifications, and cell wall. Recent reports show that treatments with small modulators of autophagy, proteases and epigenetic marks reduce cell death and enhance embryogenesis initiation in several crops, opening up new possibilities for improving in vitro embryo production in breeding programs.

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“…Over the past few years, progress of molecular techniques have immensely contributed in understanding the molecular aspects of SE in many plant species i.e. Arabidopsis [ 5 , 6 ], cotton [ 7 ], alfalfa [ 8 ], conifer [ 9 ], potato [ 10 ], Glycine max [ 11 ], oil palm [ 12 , 13 ], maize [ 14 ], Picea glauca [ 15 ], P. balfouriana [ 16 ], Vitis vinifera [ 17 ], Medicago truncatula [ 18 ] and Manihot esculenta [ 19 , 20 ]. Although the draft molecular interaction network provided for SE highlights the potential interactions at a molecular level [ 21 ], the functional characterization of the majority of genes involved in somatic-to-embryogenic transition and subsequent embryo maturation still remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Global scale transcriptome analysis of Arabidopsis embryogenesis in vitro

2015

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BackgroundSomatic embryogenesis (SE) in plants is a process by which embryos are generated directly from somatic cells, rather than from the fused products of male and female gametes. Despite the detailed expression analysis of several somatic-to-embryonic marker genes, a comprehensive understanding of SE at a molecular level is still lacking. The present study was designed to generate high resolution transcriptome datasets for early SE providing the way for future research to understand the underlying molecular mechanisms that regulate this process. We sequenced Arabidopsis thaliana somatic embryos collected from three distinct developmental time-points (5, 10 and 15 d after in vitro culture) using the Illumina HiSeq 2000 platform.ResultsThis study yielded a total of 426,001,826 sequence reads mapped to 26,520 genes in the A. thaliana reference genome. Analysis of embryonic cultures after 5 and 10 d showed differential expression of 1,195 genes; these included 778 genes that were more highly expressed after 5 d as compared to 10 d. Moreover, 1,718 genes were differentially expressed in embryonic cultures between 10 and 15 d. Our data also showed at least eight different expression patterns during early SE; the majority of genes are transcriptionally more active in embryos after 5 d. Comparison of transcriptomes derived from somatic embryos and leaf tissues revealed that at least 4,951 genes are transcriptionally more active in embryos than in the leaf; increased expression of genes involved in DNA cytosine methylation and histone deacetylation were noted in embryogenic tissues. In silico expression analysis based on microarray data found that approximately 5% of these genes are transcriptionally more active in somatic embryos than in actively dividing callus and non-dividing leaf tissues. Moreover, this identified 49 genes expressed at a higher level in somatic embryos than in other tissues. This included several genes with unknown function, as well as others related to oxidative and osmotic stress, and auxin signalling.ConclusionsThe transcriptome information provided here will form the foundation for future research on genetic and epigenetic control of plant embryogenesis at a molecular level. In follow-up studies, these data could be used to construct a regulatory network for SE; the genes more highly expressed in somatic embryos than in vegetative tissues can be considered as potential candidates to validate these networks.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1504-6) contains supplementary material, which is available to authorized users.

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Transcriptome analysis of callus from Picea balfouriana

Cited by 40 publications

References 56 publications

Long-term changes in the tree radial growth and intrinsic water-use efficiency of Chuanxi spruce (Picea likiangensis var. balfouriana) in southwestern China

Long-term changes in the tree radial growth and intrinsic water-use efficiency of Chuanxi spruce (Picea likiangensis var. balfouriana) in southwestern China

Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement

Global scale transcriptome analysis of Arabidopsis embryogenesis in vitro

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