Transcriptome analysis of Citrus sinensis reveals potential responsive events triggered by Candidatus Liberibacter asiaticus

Liu, Chang; Chang, Xiaopeng; Li, Fuxuan; Yan, Yana; Zuo, Xiru; Huang, Guiyan; Li, Ruimin

doi:10.1007/s00709-023-01911-0

Cited by 4 publications

(12 citation statements)

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“…Upon CLas infection, the citrus transcriptome undergoes significant alterations, leading to the up-regulation or down-regulation of numerous genes ( Liu et al., 2023 ). These genes are primarily associated with plant defense responses, photosynthesis, cell wall modification, nutrient transport, and metabolism among others ( Hu et al., 2017 ; Zhao et al., 2019 ; Liu et al., 2019b ; Ribeiro et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, genes related to secondary metabolite biosynthesis, like phytoalexins, are up-regulated, providing further biochemical defense against the pathogen ( Fu et al., 2016 ). CLas infection has been associated with a broad down-regulation of genes related to photosynthesis and carbohydrate metabolism ( Martinelli et al., 2012 ; Liu et al., 2019b , 2023 ). The down-regulation of these genes likely contributes to the pronounced leaf yellowing observed in CLas-infected trees.…”

Section: Introductionmentioning

confidence: 99%

“…The down-regulation of these genes likely contributes to the pronounced leaf yellowing observed in CLas-infected trees. Transcriptomic analysis has revealed an up-regulation of genes related to cell-wall modification, such as those encoding pectin methylesterases and pectate lyases ( Wang et al., 2016 ; Liu et al., 2023 ). These enzymes are involved in the modification and degradation of the cell wall, suggesting an alteration in plant cell wall integrity and structure during CLas infection.…”

Section: Introductionmentioning

confidence: 99%

“…Applications of GSEA in plant-pathogen interaction studies have led to the identification of key gene sets and pathways that are modulated during infection ( Bautista et al., 2021 ). Furthermore, the utilization of Gene ontology (GO) and Kyoto Encyclopedia of genes and genomes (KEGG) enrichment analyses enables identification of the biological processes and metabolic pathways in which differentially expressed genes (DEGs) are involved ( Liu et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

“…In order to elucidate the disturbances in biological processes within Citrus spp. triggered by CLas infection, we collected HLB associated RNA-seq data, including the RNA-seq data sequenced in our previous study ( Liu et al., 2023 ) and public RNA-seq data deposited in NCBI SRA database from various research groups, for analysis. Subsequently, we utilized WGCNA to identify gene sets and pinpointed hub genes positive correlated with CLas infection.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of huanglongbing-associated RNA-seq data reveals disturbances in biological processes within Citrus spp. triggered by Candidatus Liberibacter asiaticus infection

Li,

Wang,

et al. 2024

Front. Plant Sci.

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IntroductionHuanglongbing (HLB), a disease that’s ubiquitous worldwide, wreaks havoc on the citrus industry. The primary culprit of HLB is the gram-negative bacterium Candidatus Liberibacter asiaticus (CLas) that infects the phloem, but its damaging mechanism is yet to be fully understood.Methods and resultsIn this study, a multitude of tools including weighted correlation network analysis (WGCNA), protein-protein interaction (PPI) network analysis and gene expression profiling are employed to unravel the intricacies of its pathogenesis. The investigation pinpoints various central genes, such as the ethylene-responsive transcription factor 9 (ERF9) and thioredoxin reductase 1 (TrxR1), that are associated with CLas invasion and resultant disturbances in numerous biological operations. Additionally, the study uncovers a range of responses through the detection of differential expressed genes (DEGs) across different experiments. The discovery of core DEGs leads to the identification of pivotal genes such as the sieve element occlusion (SEO) and the wall-associated receptor kinase-like 15 (WAKL15). PPI network analysis highlights potential vital proteins, while GO and KEGG pathway enrichment analysis illustrate a significant impact on multiple defensive and metabolic pathways. Gene set enrichment analysis (GSEA) indicates significant alterations in biological processes such as leaf senescence and response to biotic stimuli.DiscussionThis all-encompassing approach extends valuable understanding into the pathogenesis of CLas, potentially aiding future research and therapeutic strategies for HLB.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of huanglongbing-associated RNA-seq data reveals disturbances in biological processes within Citrus spp. triggered by Candidatus Liberibacter asiaticus infection

Li,

Wang,

et al. 2024

Front. Plant Sci.

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Taming the fire—transcription factors for redox control in animals and plants

Nick

2024

Protoplasma

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SDE19, a SEC-dependent effector from ‘Candidatus Liberibacter asiaticus’ suppresses plant immunity and targets Citrus sinensis Sec12 to interfere with vesicle trafficking

Huang,

Chang,

et al. 2024

PLoS Pathog

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Citrus huanglongbing (HLB), which is caused by the phloem-colonizing bacteria Candidatus Liberibacter asiaticus (CLas), poses a significant threat to citrus production worldwide. The pathogenicity mechanism of HLB remains poorly understood. SEC-dependent effectors (SDEs) have been suggested to play critical roles in the interaction between citrus and CLas. Here, we explored the function of CLIBASIA_05320 (SDE19), a core SDE from CLas, and its interaction with its host target. Our data revealed that SDE19 is expressed at higher level during infection of citrus than that during infection of the Asian citrus psyllid. Subcellular localization assays showed that SDE19 is localized in the nucleus and cytoplasm and is capable of moving from cell to cell in Nicotiana benthamiana. To investigate whether SDE19 facilitates pathogen infection, we generated transgenic Arabidopsis thaliana and citrus plants overexpressing SDE19. Transgenic A. thaliana and citrus plants were more susceptible to Pseudomonas syringae pv. tomato (Pst) and Xanthomonas citri subsp. citri (Xcc), respectively. In addition, RNA-seq analysis demonstrated that overexpression of SDE19 resulted in a reprogramming of expression of genes related to biotic stimulus responses. SDE19 interacts with Citrus sinensis Sec12, a guanine nucleotide exchange factor responsible for the assembly of plant COPII (coat protein II)-coated vesicles, which mediate vesicle trafficking from the ER to the Golgi. SDE19 colocalizes with Sec12 in the ER by binding to its N-terminal catalytic region, affecting the stability of Sec12 through the 26S proteasome. This interaction hinders the secretion of apoplastic defense-related proteins such as PR1, P69B, GmGIP1, and RCR3. Furthermore, the secretion of PR1 and callose deposition is decreased in SDE19-transgenic A. thaliana. Taken together, SDE19 is a novel virulent SDE secreted by CLas that interacts with Sec12 to disrupt vesicle trafficking, inhibit defense-related proteins secretion, and promote bacterial infection. This study sheds light on how CLas manipulates the host vesicle trafficking pathway to suppress the secretion of defense-related proteins and interfere with plant immunity.

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Transcriptome analysis of Citrus sinensis reveals potential responsive events triggered by Candidatus Liberibacter asiaticus

Cited by 4 publications

References 67 publications

Analysis of huanglongbing-associated RNA-seq data reveals disturbances in biological processes within Citrus spp. triggered by Candidatus Liberibacter asiaticus infection

Analysis of huanglongbing-associated RNA-seq data reveals disturbances in biological processes within Citrus spp. triggered by Candidatus Liberibacter asiaticus infection

Taming the fire—transcription factors for redox control in animals and plants

SDE19, a SEC-dependent effector from ‘Candidatus Liberibacter asiaticus’ suppresses plant immunity and targets Citrus sinensis Sec12 to interfere with vesicle trafficking

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