Transcriptome analysis of differentially expressed genes during anther development stages on male sterility and fertility in Cucumis melo L. line

Dai, Dongyang; Xiong, Anping; Lei, Yuan; Sheng, Yunyan; Peng, Ji; Jin, Yongfeng; Li, Dandan; Wang, Yanhong; Luan, Feishi

doi:10.1016/j.gene.2019.04.089

Cited by 11 publications

(6 citation statements)

References 61 publications

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“…Among the genes overexpressed throughout flower development, we also found several genes involved in ethylene signalling, many homologues of the cytochrome p450 family, in addition to genes of the ABC transporter family (A, B, C, D, F and I). This was in agreement with what was described in melon ( Dai et al , 2019 ). In general, the analysis of the enriched terms also gave us a broader view of the set of processes that take place during flower development, and the results seemed to indicate that metabolic and nuclear processes prevail in the early stages of development, whereas membrane-associated processes predominate in the later stages.…”

Section: Discussionsupporting

confidence: 93%

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Pechar,

Sánchez-Pina,

Coronado-Parra

et al. 2023

Annals of Botany

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Background and Aims Given the lack of specific studies on floral development in melon (Cucumis melo L.), we carried out an extensive study involving morphological and transcriptomic analyses to characterize floral development in this species. Methods Using an andromonoecious line, we analysed the development of floral buds in male and hermaphrodite flowers with both light microscopy and scanning electron microscopy. Based on flower lengths, we established a correlation between the developmental stages and four main episodes of floral development and conducted an extensive RNA sequencing analysis of these episodes. Key Results We identified 12 stages of floral development, from the appearance of the floral meristems to anthesis. The main structural differences between male and hermaphrodite flowers appeared between stages 6 and 7; later stages of development leading to the formation of organs and structures in both types of flowers were also described. We analysed the gene expression patterns of the four episodes in flower development to find the genes that were specific to each given episode. Among others, we identified genes that defined the passage from one episode to the next according to the ABCDE model of floral development. Conclusions This work combines a detailed morphological analysis and a comprehensive transcriptomic study to enable characterization of the structural and molecular mechanisms that determine the floral development of an andromonoecious genotype in melon. Taken together, our results provide a first insight into gene regulation networks in melon floral development that are crucial for flowering and pollen formation, highlighting potential targets for genetic manipulation to improve crop yield of melon in the future.

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Section: Discussionsupporting

confidence: 93%

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Pechar,

Sánchez-Pina,

Coronado-Parra

et al. 2023

Annals of Botany

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“…In recent years, advancement in molecular technology, has enabled breeders and molecular researchers to identify various plant transcription factors, genes and explore protein expression at the translational, transcriptome and proteome level, such as the CMS studies of the Chinese cabbage (5) , turnip (6) , Cucumis melo L. (7) , cotton (8, 9) , rice (10) , Brassica napus L. (11) . Transcriptomic analysis in cotton (CMS-D8) revealed that the reactive oxygen species (ROS) were released from mitochondria and served as important signal molecules in the nucleus, causing the formation of abnormal tapetum (8) .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Proteomic Analyses of a New Cytoplasmic Male Sterile Line with wildGossypium bickii.Genetic Background

Zhao

Wang

et al. 2020

Preprint

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39Cotton is an important fiber crop but has serious effects of heterosis, in which cytoplasmic male 40 sterility (CMS) being the major cause of heterosis in plants. However, there are no studies done on 41 CMS Yamian A in cotton with the genetic background of the Australian wild Gossypium bickii. 42 Transcriptomic and proteomic results showed that UDP-glucosyltransferase -in the nucleus, 60S 43 ribosomal protein L13a-in the cytoplasm, ribulose-1,5-bisphosphate carboxylase/oxygenase -in the 44 chloroplast, glutathione S-transferase -in the cytoplasm, and ATP synthase F1 subunit 1 -in the 45 mitochondrion were upregulated; while low molecular weight heat shock protein -in the chloroplast 46 and ATP synthase D chain-in the mitochondrion were down-regulated expression at the microspore 47 58 Cotton is an important cash crop with high-quality fiber, edible oil, and protein which is mainly 59 used as animal feeds (1) . Heterosis in cotton is quite apparent and has been widely used in yield quality, 60 and resistance studies in cotton (2) . The adoption of the production of hybrid seeds is the most 61 important link among some links of cotton heterosis use. At present, the castration in the production 62 of hybrid seeds often relies on hand emasculation and male-sterile lines including chemically induced 63 male sterility (IMS), genic male sterility (GMS), and cytoplasmic male sterility (CMS) (3) . The 64 production practice showed that CMS is an effective way of heterosis utilization in crop and widely 65 used to produce hybrid seeds, because it eliminates the need for artificial emasculation, saves 66 manpower and material resources, enhance the purity of hybrid seeds and increases the output of 67 crops (3, 4) . All over the world, cotton studies started in the 1960s, since then a number of germplasms 68 have been developed, such as G. arboreum L, G. harknessii Brandegee, G. trilobum (DC.) Skov., G. 69 hirsutum, G. barbadense L., among others. However, there is no report on CMS in cotton with 70 genetic background of Australian wild Gossypium bickii, which has been reported despite the 71 enormous effects of heterosis in cotton germplasm development. 72 In recent years, advancement in molecular technology, has enabled breeders and molecular 73 researchers to identify various plant transcription factors, genes and explore protein expression at the 74 translational, transcriptome and proteome level, such as the CMS studies of the Chinese cabbage (5) , 75 turnip (6) , Cucumis melo L. (7) , cotton (8,9) , rice (10) , Brassica napus L. (11) . Transcriptomic analysis in 76 5 cotton (CMS-D8) revealed that the reactive oxygen species (ROS) were released from mitochondria 77 and served as important signal molecules in the nucleus, causing the formation of abnormal 78 tapetum (8) . Proteome analyses in cotton indicated that the differentially expressed proteins (DEPs) 79 mainly involved in pyruvate, carbohydrate and fatty acid metabolism had been identified between the 80 male-sterile line 1355A a...

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“…Furthermore, the down‐regulation of genes involved in ethylene synthesis, stamen filament development and sporopollenin biosynthetic processes was consistent with what was observed by Dai et al . (2019) in the comparison with DEGs between male fertile versus male‐sterile flowers in melon (Dai et al ., 2019). In the GI episode, modifications in the expression patterns of genes related to ‘MCM complex’ and ‘DNA helicase’ may be associated with meiotic imbalances, while the down‐regulation of genes related to pectin metabolism could be due to impairments in tapetum development, as both phenomena have been associated with male sterility in Arabidopsis (Ma, 2005).…”

Section: Discussionmentioning

confidence: 99%

Editing melon eIF4E associates with virus resistance and male sterility

Pechar

Donaire

Gosálvez

et al. 2022

Plant Biotechnology Journal

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Summary The cap‐binding protein eIF4E, through its interaction with eIF4G, constitutes the core of the eIF4F complex, which plays a key role in the circularization of mRNAs and their subsequent cap‐dependent translation. In addition to its fundamental role in mRNA translation initiation, other functions have been described or suggested for eIF4E, including acting as a proviral factor and participating in sexual development. We used CRISPR/Cas9 genome editing to generate melon eif4e knockout mutant lines. Editing worked efficiently in melon, as we obtained transformed plants with a single‐nucleotide deletion in homozygosis in the first eIF4E exon already in a T0 generation. Edited and non‐transgenic plants of a segregating F2 generation were inoculated with Moroccan watermelon mosaic virus (MWMV); homozygous mutant plants showed virus resistance, while heterozygous and non‐mutant plants were infected, in agreement with our previous results with plants silenced in eIF4E. Interestingly, all homozygous edited plants of the T0 and F2 generations showed a male sterility phenotype, while crossing with wild‐type plants restored fertility, displaying a perfect correlation between the segregation of the male sterility phenotype and the segregation of the eif4e mutation. Morphological comparative analysis of melon male flowers along consecutive developmental stages showed postmeiotic abnormal development for both microsporocytes and tapetum, with clear differences in the timing of tapetum degradation in the mutant versus wild‐type. An RNA‐Seq analysis identified critical genes in pollen development that were down‐regulated in flowers of eif4e/eif4e plants, and suggested that eIF4E‐specific mRNA translation initiation is a limiting factor for male gametes formation in melon.

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Transcriptome analysis of differentially expressed genes during anther development stages on male sterility and fertility in Cucumis melo L. line

Cited by 11 publications

References 61 publications

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development

Transcriptomic and Proteomic Analyses of a New Cytoplasmic Male Sterile Line with wildGossypium bickii.Genetic Background

Editing melon eIF4E associates with virus resistance and male sterility

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