Transcriptome Analysis of Genes Involved in Dendrobine Biosynthesis in Dendrobium nobile Lindl. Infected with Mycorrhizal Fungus MF23 (Mycena sp.)

Li, Qing; Ding, Gang; Li, Biao; Guo, Shun‐Xing

doi:10.1038/s41598-017-00445-9

Cited by 72 publications

(95 citation statements)

References 52 publications

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“…Employing transcriptomic approaches to collect and analyze bioinformation for understanding its genetic makeup and biosynthetic pathways of important secondary metabolites is a practical way to better protect and utilize this medicinal resource by using biotechnologies to produce products of interest in the future. By far, studies on genomic and transcriptomic level of Dendrobium plants have been confined to a few species such as D. officinal and D. nobile [13,15,16]. To our best knowledge, no transcriptomic report on D. huoshanense was retrieved up to date.…”

Section: Digital Signaturementioning

confidence: 97%

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Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis

et al. 2018

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Dendrobium huoshanense has long been used to treat various diseases in oriental medicine. In order to study its gene expression profile, transcripts involved in the biosynthesis of precursors of polysaccharides, as well as mechanisms underlining morphological differences between wild and cultivated plants, three organs of both wild type and cultivated D. huoshanense were collected and sequenced by Illumina HiSeq4000 platform, yielding 919,409,540 raw reads in FASTQ format. After Trinity de novo assembly and quality control, 241,242 nonredundant contigs with the average length of 967.5 bp were generated. qRT-PCR experiment on the selected transcripts showed the transcriptomic data were reliable. BLASTx was conducted against NR, SwissProt, String, Pfam, and KEGG. Gene ontology annotation revealed more than 40,000 contigs assigned to catalytic activity and metabolic process, suggesting its dynamic physiological activities. By searching KEGG pathway, six genes potentially involved in mannose biosynthetic pathway were retrieved. Gene expression analysis for stems between wild and cultivated D. huoshanense resulted in 956 genes differentially expressed. Simple sequence repeats (SSRs) analysis revealed 143 SSRs with the unit size of 4 and 3,437 SSRs the size of 3. The obtained SSRs are the potential molecular markers for discriminating distinct cultivars of D. huoshanense.

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Section: Digital Signaturementioning

confidence: 97%

“…Moreover, dendrobine also exhibits antipyretic, hypertensive, and antiviral effects [8]. Although several groups have reported racemic and enantioselective syntheses for this compound during the past five decades [9][10][11][12], study on its biosynthetic pathway remains limited [13,14].…”

Section: Digital Signaturementioning

confidence: 99%

Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis

et al. 2018

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“…The symbiosis between Dendrobium nobile and Mycena sp. MF23 caused the accumulation of dendrobine and polysaccharide [14,15]. Mycena sp.…”

Section: Introductionmentioning

confidence: 99%

Combined Metabolome and Transcriptome Analyses Reveal the Effects of Mycorrhizal Fungus Ceratobasidium sp. AR2 on the Flavonoid Accumulation in Anoectochilus roxburghii during Different Growth Stages

Zhang

Chen

et al. 2020

IJMS

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Anoectochilus roxburghii is a traditional Chinese herb with high medicinal value, with main bioactive constituents which are flavonoids. It commonly associates with mycorrhizal fungi for its growth and development. Moreover, mycorrhizal fungi can induce changes in the internal metabolism of host plants. However, its role in the flavonoid accumulation in A. roxburghii at different growth stages is not well studied. In this study, combined metabolome and transcriptome analyses were performed to investigate the metabolic and transcriptional profiling in mycorrhizal A. roxburghii (M) and non-mycorrhizal A. roxburghii (NM) growth for six months. An association analysis revealed that flavonoid biosynthetic pathway presented significant differences between the M and NM. Additionally, the structural genes related to flavonoid synthesis and different flavonoid metabolites in both groups over a period of six months were validated using quantitative real-time polymerase chain reaction (qRT-PCR) and high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The results showed that Ceratobasidium sp. AR2 could increase the accumulation of five flavonol-glycosides (i.e., narcissin, rutin, isorhamnetin-3-O-beta-d-glucoside, quercetin-7-O-glucoside, and kaempferol-3-O-glucoside), two flavonols (i.e., quercetin and isorhamnetin), and two flavones (i.e., nobiletin and tangeretin) to some degrees. The qRT-PCR showed that the flavonoid biosynthetic genes (PAL, 4CL, CHS, GT, and RT) were significantly differentially expressed between the M and NM. Overall, our findings indicate that AR2 induces flavonoid metabolism in A. roxburghii during different growth stages, especially in the third month. This shows great potential of Ceratobasidium sp. AR2 for the quality improvement of A. roxburghii.

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“…The active medicinal ingredients in D. officinale include: polysaccharides, alkaloids, phenols, terpenes, flavonoids and bibenzyl [9][10]. In Dendrobium plants, the content of sesquiterpene alkaloid is the main measure of its quality and medicinal efficacy [11][12]. Notably, previous studies have found that bibenzyl compounds (belonging to sesquiterpene alkaloids) might be the only bioactive ingredients in D. officinale [13][14].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, based on transcriptomic analysis, several putative rate-limiting enzyme genes responsible for the biosynthesis of terpenoids in Eugenia uniflora [32], lignin in Apium graveolens [33], and flavonoid in Phyllanthus emblica, Dracaena cambodiana, and Solanum viarum [34][35][36] have been identified. For Dendrobium plants, several studies have reported on flavonoid biosynthetic pathway analysis and the gene mining of key enzymes [37] as well as alkaloid biosynthetic pathway analysis and the identification of key enzyme genes [12,[38][39]. However, the bibenzyl biosynthetic pathway and the potential genes responsible for regulating bibenzyl biosynthesis in Dendrobium plants remain underexplored.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analyses reveal putative genes associated with bibenzyl biosynthesis in the traditional Chinese herb Dendrobium officinale

Oi¹,

Guan²,

Yang³

et al. 2019

Preprint

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Background: Dendrobium plants are well known for their uses in traditional Chinese herbal medicine. Bibenzyl compounds are the main active compounds in Dendrobium officinale. However, the physiological and molecular basis for the biosynthesis of bibenzyl compounds in Dendrobium plants remains underexplored. Results: In this study, the accumulation of erianin and gigantol were studied as representative compounds of bibenzyl. Their presence in plant tissues were investigated. Our results show that root tissues contained the highest content of bibenzyl (erianin and gigantol). Based on the pre-experimental result that exogenous application of Methyl-Jasmonate promotes the biosynthesis of bibenzyl compounds in D. officinale root tissues, comparative transcriptomic analyses were conducted between the bibenzyl-accumulated root tissues and a control. In total, we identified 1,342 differentially expressed genes (DEGs) with 912 up-regulated and 430 down-regulated genes. Most of the identified DEGs are functionally involved in the JA signaling pathway and the biosynthesis of secondary metabolites. In particular, we identified 11 enzymatic genes functionally involved in bibenzyl biosynthesis.Conclusions: Our study provide insights on the identification of putative genes associated with bibenzyl biosynthesis and accumulation in Dendrobium plants, and also paves the way for future research on dissecting the physiological and molecular mechanisms of bibenzyl synthesis in plants as well as on how to best utilize genetic engineering and molecular modification techniques to genetically improve Dendrobium varieties by increasing the content of bibenzyl for drug production and industrialization. Keywords: Bibenzyl, D. officinale , Differentially expressed genes, Secondary metabolites, Transcriptome analysis.

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Transcriptome Analysis of Genes Involved in Dendrobine Biosynthesis in Dendrobium nobile Lindl. Infected with Mycorrhizal Fungus MF23 (Mycena sp.)

Cited by 72 publications

References 52 publications

Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis

Transcriptomic landscape of Dendrobium huoshanense and its genes related to polysaccharide biosynthesis

Combined Metabolome and Transcriptome Analyses Reveal the Effects of Mycorrhizal Fungus Ceratobasidium sp. AR2 on the Flavonoid Accumulation in Anoectochilus roxburghii during Different Growth Stages

Transcriptomic analyses reveal putative genes associated with bibenzyl biosynthesis in the traditional Chinese herb Dendrobium officinale

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