Transcriptome analysis of Grapholitha molesta (Busk) (Lepidoptera: Tortricidae) larvae in response to entomopathogenic fungi Beauveria bassiana

Cao, Zhishan; Cao, Jinjun; Власенко, В. А.; Wang, Xinfa; Li, Weihai

doi:10.1016/j.aspen.2022.101926

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…Based on the de novo transcriptome sequence in the database constructed previously by our library, the full‐length cDNA of GmBGRP was obtained (Cao et al, 2022). To verify the full‐length cDNA acquired from the transcriptome sequences, two gene‐specific primers, BGBP‐F/R1 and BGRP ‐F/R2 (Table 1), were used to amplify the opening reading frame (ORF) of GmBGRP .…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization and functional analysis of a beta‐1,3‐glucan recognition protein from oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Cao,

Vlasenko

et al. 2023

Arch Insect Biochem Physiol

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The beta‐1,3‐glucan recognition protein (BGRP) is an important pattern recognition protein (PRP), which plays an important role in immune recognition and signaling pathway of insect innate immunity. Herein, a BGRP gene was obtained from the transcriptome of Grapholita molesta and its expression was verified by PCR. The full cDNA of the GmBGRP gene was 1691 bp encoding 486 amino acid residues. The calculated molecular mass of the mature protein was 54.83 kDa with an estimated pI of 6.14. The amino acid sequence of GmBGRP was highly homologous to BGRPs of other lepidopterans including Leguminivora glycinivorella BGRP‐3. Expression profile of GmBGRP at different developmental stages and different tissues of 5th instar larvae showed that the expression level of this gene tends to slightly increase and then decrease at the adult stage, with the highest at the pupa stage; and mainly expressed in the epidermis, fat body and hemocytes compared with other tissues. In addition, we investigated the transcription levels of other immune‐related genes, such as Serine‐1, Serine‐2, Serine‐3, Serpin, SRCB (scavenger receptor gene), Toll, PPO (prophenoloxidase) upon GmBGRP gene silencing, indicating that GmBGRP expression is associated with immune responses of G. molesta. This was further supported by the upregulation of the mRNA level of GmBGRP following fungal infection. Taken together, these results provide experimental evidence for the role of GmBGRP gene in immune defense in G. molesta larvae.

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Section: Methodsmentioning

confidence: 99%

Molecular characterization and functional analysis of a beta‐1,3‐glucan recognition protein from oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Cao,

Vlasenko

et al. 2023

Arch Insect Biochem Physiol

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“…All the samples were sequenced on an Illumina HiSeq TM 2500 platform (Illumina, San Diego, CA, USA). The original data were stored in FASTQ format [ 44 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

Preliminary Analysis of Transcriptome Response of Dioryctria sylvestrella (Lepidoptera: Pyralidae) Larvae Infected with Beauveria bassiana under Short-Term Starvation

Guo

Jia

Chen

et al. 2023

Insects

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The Dioryctria genus contains several destructive borer pests that are found in coniferous forests in the Northern Hemisphere. Beauveria bassiana spore powder was tested as a new method of pest control. In this study, Dioryctria sylvestrella (Lepidoptera: Pyralidae) was used as the object. A transcriptome analysis was performed on a freshly caught group, a fasting treatment control group, and a treatment group inoculated with a wild B. bassiana strain, SBM-03. Under the conditions of 72-h fasting and a low temperature of 16 ± 1 °C, (i) in the control group, 13,135 of 16,969 genes were downregulated. However, in the treatment group, 14,558 of 16,665 genes were upregulated. (ii) In the control group, the expression of most genes in the upstream and midstream of the Toll and IMD pathways was downregulated, but 13 of the 21 antimicrobial peptides were still upregulated. In the treatment group, the gene expression of almost all antimicrobial peptides was increased. Several AMPs, including cecropin, gloverin, and gallerimycin, may have a specific inhibitory effect on B. bassiana. (iii) In the treatment group, one gene in the glutathione S-transferase system and four genes in the cytochrome P450 enzyme family were upregulated, with a sharp rise in those that were upregulated significantly. In addition, most genes of the peroxidase and catalase families, but none of the superoxide dismutase family were upregulated significantly. Through innovative fasting and lower temperature control, we have a certain understanding of the specific defense mechanism by which D. sylvestrella larvae may resist B. bassiana in the pre-wintering period. This study paves the way for improving the toxicity of B. bassiana to Dioryctria spp.

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Transcriptome analysis of Grapholitha molesta (Busk) (Lepidoptera: Tortricidae) larvae in response to entomopathogenic fungi Beauveria bassiana

Cited by 2 publications

References 40 publications

Molecular characterization and functional analysis of a beta‐1,3‐glucan recognition protein from oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Molecular characterization and functional analysis of a beta‐1,3‐glucan recognition protein from oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae)

Preliminary Analysis of Transcriptome Response of Dioryctria sylvestrella (Lepidoptera: Pyralidae) Larvae Infected with Beauveria bassiana under Short-Term Starvation

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