Transcriptome Analysis of Needle and Root of Pinus Massoniana in Response to Continuous Drought Stress

Xiao, Feng; Yang, Zhao; Wang, Xiurong; Li, Qiao; Ran, Jie

doi:10.3390/plants10040769

Cited by 20 publications

(16 citation statements)

References 56 publications

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“…Soluble protein content was measured by Coomassie's brilliant blue staining (Goharrizi et al, 2021). MDA content was measured by the TBA method and the root activity was measured by the 2,3,5-Triphenyte-trazoliumchloride (TTC) method (Xiao et al, 2021). Photosynthesis and fluorescence-related indexes, namely, net photosynthetic rate (Pn), transpiration rate (E), stomatal conductance (gtc), intercellular carbon dioxide concentration (Ci), instant water use efficiency (iWUE), and maximal quantum yield of PSII (Fv/Fm), PSII actual photochemical quantum efficiency (ϕPSII), electron transport rate (ETR), photochemical quenching (qP), and non-photochemical quenching (NPQ), were measured by an LI-6,800 photosynthesis measurement system (LI-COR, United States).…”

Section: Data Collectionmentioning

confidence: 99%

Astragalus sinicus Incorporated as Green Manure for Weed Control in Corn

Liu

Zhang

et al. 2022

Front. Plant Sci.

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Astragalus sinicus L. (milk vetch), one of the most widespread green manure species, is widely planted in the temperate zone. Eleusine indica L. (goosegrass), a serious annual weed in the world, has evolved resistance to some non-selective herbicides. The use of milk vetch as green manure for weed control in paddy fields was proposed. Aqueous extracts of milk vetch are known to exert a different level of phytotoxicity on weeds and crops. Phytotoxic substances contained in green manure were released into the soil by leaching at the initial stage and decomposition at the later stage after the return of green manure. Considering the need for searching new sustainable strategies for weed control, a question arises: “if milk vetch could be applied in goosegrass control, which stage is the most important to control goosegrass after milk vetch returned to the field, and at the same time, will the subsequent crop, corn (Zea mays L.), be affected by the side effects from milk vetch phytotoxicity?” In this study, the potential of milk vetch for goosegrass control was approached by repeated laboratory experiments, which include the aqueous extract experiment, decomposed experiment, and pot experiment. The effects of milk vetch returning to the field on maize were simulated by a pot experiment. The extract of milk vetch could significantly inhibit the germination of goosegrass at 2% concentration, and the inhibition enhanced with the increase of concentration. In the decomposed liquid experiment, decay time within 15 days, with the increase of decay days or concentration, goosegrass inhibition effect of decomposed liquid was enhanced. When decay time was more than 15 days, the inhibition ability of the decomposed liquid to goosegrass decreased. According to the RI accumulated value, aqueous extract and decomposed liquid have a “hormesis effect” on the germination and growth of goosegrass. Pot experiment proved that the addition of 1–10% (w/w) of milk vetch significantly reduced the germination and growth of goosegrass. On the contrary, the comprehensive analysis showed that the participation of milk vetch was conducive to the growth of corn. Our results constitute evidence that the incorporation of milk vetch into the soil could be a feasible practice to reduce weed infarctions in the corn-based cropping system.

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Section: Data Collectionmentioning

confidence: 99%

Astragalus sinicus Incorporated as Green Manure for Weed Control in Corn

Liu

Zhang

et al. 2022

Front. Plant Sci.

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“…Drought stress limits root growth and inhibits cytokinin production (Xu et al 2016). As a result, MDA content in plant roots continues to increase, root activity continues to decline, and POD may contribute to production of ROS, thereby improving the antioxidant defence system (Xiao et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Sophora tonkinensis: response and adaptation of physiological characteristics, functional traits, and secondary metabolites to drought stress

Liang,

Wei,

Qin

et al. 2023

Plant Biol J

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The medicinal plant Sophora tonkinensis is a characteristic Chinese shrub of karst areas. The arid climate in karst areas produces high‐quality S. tonkinensis; however, the mechanisms of drought tolerance are not clear, which restricts sustainable plantings of S. tonkinensis. This study involved a 20‐day drought stress experiment with potted S. tonkinensis and threee soil water regimes: control (CK), mild drought (MDT), and severe drought (SDT). Plant morphology, biomass, physiological indicators, alkaloid content, and other changes under drought stress were monitored. The content of soluble sugars and proteins, and activity of antioxidant enzymes in leaves and roots were higher under drought than CK, indicating that S. tonkinensis is tolerant to osmotic stress in early drought stages. Content of matrine and oxymatrine increased gradually with increasing drought duration in the short term. The epidermis of S. tonkinensis leaves have characteristics of desert plants, including upper epidermal waxy layer, lower epidermal villi, and relatively sunken stomata, suggesting that S. tonkinensis has strong drought tolerance. In conclusion, drought stress changed the cell structure of S. tonkinensis, induced antioxidant enzyme activity and increased its resistance to drought.

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“…Differentiation in drought tolerance of crop species has long been recognized to reflect their genetic basis ( Thabet et al., 2018 ; Dossa et al., 2019 ; Mahmood et al., 2019 ), thus coniferous species also have their genetic basis for differentiation in drought tolerance. Because of this reason, recently substantial effort has been devoted to understanding genetic basis in differential drought tolerance of coniferous species and other forest trees using molecular and genomic tools ( Haas et al., 2021 ; Xiao et al., 2021 ; Li M. et al., 2022 ). The loblolly pine genome has been completely sequenced ( Neale et al., 2014 ; Zimin et al., 2014 ; Zimin et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Targeted and untargeted metabolomics reveals deep analysis of drought stress responses in needles and roots of Pinus taeda seedlings

2023

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Drought stress is one of major environmental stresses affecting plant growth and yield. Although Pinus taeda trees are planted in rainy southern China, local drought sometime occurs and can last several months, further affecting their growth and resin production. In this study, P. taeda seedlings were treated with long-term drought (42 d), and then targeted and untargeted metabolomics analysis were carried out to evaluate drought tolerance of P. taeda. Targeted metabolomics analysis showed that levels of some sugars, phytohormones, and amino acids significantly increased in the roots and needles of water-stressed (WS) P. taeda seedlings, compared with well-watered (WW) pine seedlings. These metabolites included sucrose in pine roots, the phytohormones abscisic acid and sacylic acid in pine needles, the phytohormone gibberellin (GA4) and the two amino acids, glycine and asparagine, in WS pine roots. Compared with WW pine seedlings, the neurotransmitter acetylcholine significantly increased in needles of WS pine seedlings, but significantly reduced in their roots. The neurotransmitters L-glutamine and hydroxytyramine significantly increased in roots and needles of WS pine seedlings, respectively, compared with WW pine seedlings, but the neurotransmitter noradrenaline significantly reduced in needles of WS pine seedlings. Levels of some unsaturated fatty acids significantly reduced in roots or needles of WS pine seedlings, compared with WW pine seedlings, such as linoleic acid, oleic acid, myristelaidic acid, myristoleic acid in WS pine roots, and palmitelaidic acid, erucic acid, and alpha-linolenic acid in WS pine needles. However, three saturated fatty acids significantly increased in WS pine seedlings, i.e., dodecanoic acid in WS pine needles, tricosanoic acid and heptadecanoic acid in WS pine roots. Untargeted metabolomics analysis showed that levels of some metabolites increased in WS pine seedlings, especially sugars, long-chain lipids, flavonoids, and terpenoids. A few of specific metabolites increased greatly, such as androsin, piceatanol, and panaxatriol in roots and needles of WS pine seedlings. Comparing with WW pine seedlings, it was found that the most enriched pathways in WS pine needles included flavone and flavonol biosynthesis, ABC transporters, diterpenoid biosynthesis, plant hormone signal transduction, and flavonoid biosynthesis; in WS pine roots, the most enriched pathways included tryptophan metabolism, caffeine metabolism, sesquiterpenoid and triterpenoid biosynthesis, plant hormone signal transduction, biosynthesis of phenylalanine, tyrosine, and tryptophan. Under long-term drought stress, P. taeda seedlings showed their own metabolomics characteristics, and some new metabolites and biosynthesis pathways were found, providing a guideline for breeding drought-tolerant cultivars of P. taeda.

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Transcriptome Analysis of Needle and Root of Pinus Massoniana in Response to Continuous Drought Stress

Cited by 20 publications

References 56 publications

Astragalus sinicus Incorporated as Green Manure for Weed Control in Corn

Astragalus sinicus Incorporated as Green Manure for Weed Control in Corn

Sophora tonkinensis: response and adaptation of physiological characteristics, functional traits, and secondary metabolites to drought stress

Targeted and untargeted metabolomics reveals deep analysis of drought stress responses in needles and roots of Pinus taeda seedlings

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