Transcriptome analysis of the key role of GAT2 gene in the hyper-accumulation of copper in the oyster Crassostrea angulata

Shi, Bo; Huang, Zekun; Xiang, Xu; Huang, Meng; Wang, Wen‐Xiong; Ke, Caihuan

doi:10.1038/srep17751

Cited by 34 publications

(19 citation statements)

References 57 publications

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“…The Cu, Zn, Mn, Cd and Pb concentrations of oyster tissues were measured using inductively coupled plasma‐mass spectrometry (ICP‐MS‐7700, Agilent, US) as described by Shi et al (). Approximately 100 mg powdered sample was digested with 2 ml 70% nitric acid (CNW, Germany) at room temperature for 12 hr and heated to 80°C for 12 hr.…”

Section: Methodsmentioning

confidence: 99%

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Influence of gonadal development on metal accumulation in the Portuguese oyster,Crassostrea angulata,in subtropical areas

Shi

Zhou

et al. 2019

Aquac Res

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Metal enrichment makes oyster considered to be an appropriate food source for trace elements, but excessive accumulation can threaten human health. Reproduction is an important biotic factor that affects the metal accumulation in oysters. However, the influence of continuous gonad development model on metal accumulation in some subtropical oysters is still unclear. The present study addresses the concentrations of trace metals and glycogen associated with the gonadal and somatic tissues throughout the reproductive cycle of diploid and triploid Crassostrea angulata cultured in Fujian, China. Cu, Zn and Pb contents of gonads were negatively correlated (p < 0.05) with gonadosomatic index (GSI). The correlation between Cd content and GSI was not significant. There was a positive correlation (p < 0.05) between GSI and Mn content in female gonads while the correlation was negative (p < 0.05) in males, indicating Mn plays an important role in gametogenesis of females. The effects of gonadal maturity and gender on metal accumulation in somatic tissue were not significant (p > 0.05). The concentrations of all metals in sterile triploids were not significantly different from those of the somatic tissue of diploids. Gonadal glycogen content showed a negative correlation with the GSI. However, somatic glycogen content was not affected by gonadal development, indicating that the energy consumed during gametogenesis comes mainly from recently ingested food rather than from energy stored in somatic tissue. The effects of the gonadal development on the metal accumulation in oysters varied according to the metal and tissue type.

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Section: Methodsmentioning

confidence: 99%

“…The Cu, Zn, Mn, Cd and Pb concentrations of oyster tissues were measured using inductively coupled plasma-mass spectrometry (ICP-MS-7700, Agilent, US) as described by Shi et al (2015).…”

Section: Metal Analysismentioning

confidence: 99%

Influence of gonadal development on metal accumulation in the Portuguese oyster,Crassostrea angulata,in subtropical areas

Shi

Zhou

et al. 2019

Aquac Res

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“…Numerous studies focused on metal transporters in both plants and animals have been reported [19,23,39,40]. ATP-binding cassette (ABC) family transporters are important heavy metal transporters and interact with a wide range of chemicals including metals by pumping them across the cell membrane to maintain of cellular metal homeostasis [41,42]. In our study, the expression levels of ABC transporters were inhibited or induced.…”

Section: Resultsmentioning

confidence: 66%

Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure

Wang

et al. 2016

IJMS

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Pardosa pseudoannulata is one of the most common wandering spiders in agricultural fields and a potentially good bioindicator for heavy metal contamination. However, little is known about the mechanisms by which spiders respond to heavy metals at the molecular level. In the present study, high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and to identify differentially expressed genes (DEGs) after cadmium exposure. We obtained 60,489 assembled unigenes, 18,773 of which were annotated in the public databases. A total of 2939 and 2491 DEGs were detected between the libraries of two Cd-treated groups and the control. Functional enrichment analysis revealed that metabolism processes and digestive system function were predominately enriched in response to Cd stress. At the cellular and molecular levels, significantly enriched pathways in lysosomes and phagosomes as well as replication, recombination and repair demonstrated that oxidative damage resulted from Cd exposure. Based on the selected DEGs, certain critical genes involved in defence and detoxification were analysed. These results may elucidate the molecular mechanisms underlying spiders’ responses to heavy metal stress.

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“…Gene expression in response to accumulation of copper in Crassostrea angulate was studied by Shi et al . (). This study elucidated the significance of GABA transporter 2 (GAT2) genes in copper accumulation.…”

Section: Current Status Of Transcriptomics In Aquaculturementioning

confidence: 97%

Transcriptomics in aquaculture: current status and applications

Chandhini

Kumar

2018

Reviews in Aquaculture

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Transcriptomics is the analysis of total RNA transcript in a cell, which gives a broad idea of cellular processes that are active and dormant. Recent advancement in next-generation sequencing technologies like high-throughput mRNA sequencing (RNA-seq) facilitates in deciphering the functional complexity of the whole transcriptome of an organism. In the recent past, transcriptome profiling has been widely used in aquaculture for effective identification and expression analysis of candidate genes involved in growth, reproduction, development, immunity, disease, stress and toxicology. On the paucity of a comprehensive genome sequence, transcriptomics enables the detection of molecular markers such as simple sequence repeats and single-nucleotide polymorphism associated with the trait of interest. This review summarizes the process of transcriptome analysis and the current state of transcriptomics in aquaculture related to diseases and immunity, reproduction and development, growth and nutrition, toxicology and stress. Further, this review highlights the application of transcriptomics, including generation of genetic resources, differential gene expression, analysis of molecular pathways and development of molecular markers.

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Transcriptome analysis of the key role of GAT2 gene in the hyper-accumulation of copper in the oyster Crassostrea angulata

Cited by 34 publications

References 57 publications

Influence of gonadal development on metal accumulation in the Portuguese oyster,Crassostrea angulata,in subtropical areas

Influence of gonadal development on metal accumulation in the Portuguese oyster,Crassostrea angulata,in subtropical areas

Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure

Transcriptomics in aquaculture: current status and applications

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