Transcriptome Analysis of the Spleen Provides Insight into the Immune Regulation of GCRV Resistance in Grass Carp (Ctenopharyngodon idella)

Yu, Chengchen; Wu, Minxian; Jiang, Yuchen; Xu, Xiaoyan; Li, Jiale; Shen, Yubang

doi:10.1007/s10126-023-10225-4

Cited by 8 publications

(3 citation statements)

References 42 publications

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“…For instance, Wang et al [18] found that 5 days after GCRV infection, grass carp demonstrated a significant increase in DEGs associated with PRRs, cytokines, phagosomes, lysosomes, and other pathways. Moreover, secondary infection of GCRV-resistant grass carp demonstrated that 5 days after GCRV infection, pathways related to antigen processing and presentation, toll-like receptor (TLR) signaling, and natural killer cell-mediated cytotoxicity were significantly activated [27]. These findings suggest that the grass carp immune system exhibits a robust immune response, particularly around GCRV infection day 5.…”

Section: Discussionmentioning

confidence: 92%

Comparative Transcriptomics Analysis Reveals Unique Immune Response to Grass Carp Reovirus Infection in Barbel Chub (Squaliobarbus curriculus)

Huang,

Wang,

et al. 2024

Biology

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Grass carp (Ctenopharyngodon idella) and barbel chub (Squaliobarbus curriculus)—both Leuciscinae subfamily species—demonstrate differences in grass carp reovirus (GCRV) infection resistance. We infected barbel chubs with type II GCRV and subjected their liver, spleen, head kidney, and trunk kidney samples to investigate anti-GCRV immune mechanisms via RNA sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). We identified 139, 970, 867, and 2374 differentially expressed genes (DEGs) in the liver, spleen, head kidney, and trunk kidney, respectively. Across all four tissues, gene ontology analysis revealed significant immune response-related DEG enrichment, and the Kyoto Encyclopedia of Genes and Genomes analysis revealed pattern recognition receptor (PRR) and cytokine-related pathway enrichment. We noted autophagy pathway enrichment in the spleen, head kidney, and trunk kidney; apoptosis pathway enrichment in the spleen and trunk kidney; and complement- and coagulation-cascade pathway enrichment in only the spleen. Comparative transcriptome analysis between GCRV-infected barbel chubs and uninfected barbel chubs comprehensively revealed that PRR, cytokine-related, complement- and coagulation-cascade, apoptosis, and autophagy pathways are potential key factors influencing barbel chub resistance to GCRV infection. qRT-PCR validation of 11 immune-related DEGs confirmed our RNA-seq data’s accuracy. These findings provide a theoretical foundation and empirical evidence for the understanding of GCRV infection resistance in barbel chub and hybrid grass carp–barbel chub breeding.

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Section: Discussionmentioning

confidence: 92%

Comparative Transcriptomics Analysis Reveals Unique Immune Response to Grass Carp Reovirus Infection in Barbel Chub (Squaliobarbus curriculus)

Huang,

Wang,

et al. 2024

Biology

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“…Numerous studies have investigated virus-induced immune responses in grass carp, ranging from genomic to transcriptomic levels [13][14][15][16]. During GCRV infection, teleost fish rely on pattern recognition receptors (PRRs), primarily Toll-like receptors (TLRs) and RIG-Ilike receptors (RLRs), to identify viral molecular patterns (MAMPs) or viruses [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, research findings have demonstrated contrasting immune responses in grass carp that are susceptible versus resistant to GCRV infection. GCRV-resistant grass carp can activate more immune-related pathways, including antigen processing and presentation, Toll-like receptor signaling pathway, natural killer cell-mediated cytotoxicity, and Hippo signaling pathway [15]. Transcriptomic analysis of grass carp infected with GCRV at different ages showed that the immune responses were activated more rapidly in three-year-old individuals to counteract the virus invasion [21].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Kong,

Ding,

Yang

et al. 2023

IJMS

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Grass carp reovirus (GCRV), one of the most serious pathogens threatening grass carp (Ctenopharyngodon idella), can lead to grass carp hemorrhagic disease (GCHD). Currently, GCRV can be divided into three genotypes, but the comparison of their pathogenic mechanisms and the host responses remain unclear. In this study, we utilized the Ctenopharyngodon idella kidney (CIK) model infected with GCRV to conduct comparative studies on the three genotypes. We observed a cytopathic effect (CPE) in the GCRV-I and GCRV-III groups, whereas the GCRV-II group did not show any CPE. Moreover, a consistent trend in the mRNA expression levels of antiviral-related genes across all experimental groups of CIK cells was detected via qPCR and further explored through RNA-seq analysis. Importantly, GO/KEGG enrichment analysis showed that GCRV-I, -II, and -III could all activate the immune response in CIK cells, but GCRV-II induced more intense immune responses. Intriguingly, transcriptomic analysis revealed a widespread down-regulation of metabolism processes such as steroid biosynthesis, butanoate metabolism, and N-Glycan biosynthesis in infected CIK cells. Overall, our results reveal the CIK cells showed unique responses in immunity and metabolism in the three genotypes of GCRV infection. These results provide a theoretical basis for understanding the pathogenesis and prevention and control methods of GCRV.

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Whole-genome resequencing of grass carp (Ctenopharyngodon idella) for genome-wide association study on GCRV resistance

Yu,

Jiang,

Zhang

et al. 2024

Aquaculture

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Transcriptome Analysis of the Spleen Provides Insight into the Immune Regulation of GCRV Resistance in Grass Carp (Ctenopharyngodon idella)

Cited by 8 publications

References 42 publications

Comparative Transcriptomics Analysis Reveals Unique Immune Response to Grass Carp Reovirus Infection in Barbel Chub (Squaliobarbus curriculus)

Comparative Transcriptomics Analysis Reveals Unique Immune Response to Grass Carp Reovirus Infection in Barbel Chub (Squaliobarbus curriculus)

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Whole-genome resequencing of grass carp (Ctenopharyngodon idella) for genome-wide association study on GCRV resistance

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