Transcriptome analysis on fructose as the sole carbon source enhancing perylenequinones production of endophytic fungus Shiraia sp. Slf14

Liu, Zhengying; Bao, Jianying; Yang, Huilin; Zhang, Zhibin; Ren, Yan; Zhu, Du

doi:10.1007/s13205-020-02181-w

Cited by 7 publications

(3 citation statements)

References 50 publications

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“…has gained traction (Bao et al 2023 ). Therefore, multiple factors influencing the fermentation production of PQs have been explored and optimized, including the addition of diverse nitrogen and carbon sources (Liu et al 2020 ), gene editing techniques, incorporation of biotic or abiotic inducers (Du et al 2013 ), mutation breeding, and controlled variations in light (Gao et al 2018 ) and temperature conditions (Xu et al 2023 ). Despite these efforts, meeting market demand remains elusive.…”

Section: Discussionmentioning

confidence: 99%

Heat stress-induced NO enhanced perylenequinone biosynthesis of Shiraia sp. via calcium signaling pathway

Bao,

Chen,

Zhang

et al. 2024

Appl Microbiol Biotechnol

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Perylenequinones (PQs) are natural photosensitizing compounds used as photodynamic therapy, and heat stress (HS) is the main limiting factor of mycelial growth and secondary metabolism of fungi. This study aimed to unravel the impact of HS-induced Ca2+ and the calcium signaling pathway on PQ biosynthesis of Shiraia sp. Slf14(w). Meanwhile, the intricate interplay between HS-induced NO and Ca2+ and the calcium signaling pathway was investigated. The outcomes disclosed that Ca2+ and the calcium signaling pathway activated by HS could effectively enhance the production of PQs in Shiraia sp. Slf14(w). Further investigations elucidated the specific mechanism through which NO signaling molecules induced by HS act upon the Ca2+/CaM (calmodulin) signaling pathway, thus propelling PQ biosynthesis in Shiraia sp. Slf14(w). This was substantiated by decoding the downstream positioning of the CaM/CaN (calcineurin) pathway in relation to NO through comprehensive analyses encompassing transcript levels, enzyme assays, and the introduction of chemical agents. Concurrently, the engagement of Ca2+ and the calcium signaling pathway in heat shock signaling was also evidenced. The implications of our study underscore the pivotal role of HS-induced Ca2+ and the calcium signaling pathway, which not only participate in heat shock signal transduction but also play an instrumental role in promoting PQ biosynthesis. Consequently, our study not only enriches our comprehension of the mechanisms driving HS signaling transduction in fungi but also offers novel insights into the PQ synthesis paradigm within Shiraia sp. Slf14(w). Key points • The calcium signaling pathway was proposed to participate in PQ biosynthesis under HS. • HS-induced NO was revealed to act upon the calcium signaling pathway for the first time.

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Section: Discussionmentioning

confidence: 99%

Heat stress-induced NO enhanced perylenequinone biosynthesis of Shiraia sp. via calcium signaling pathway

Bao,

Chen,

Zhang

et al. 2024

Appl Microbiol Biotechnol

Self Cite

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“…sp. Slf14 grown with different carbon sources (Liu et al, 2020 ). The highest perylenequinones production was observed with fructose and the transcription of the putative perylenequinones biosynthetic genes including the Zink-finger transcription factor, and important enzymes in precursor supply were upregulated, whereas competing pathways, such as fatty acid synthesis were downregulated.…”

Section: Successful Elucidation Of Biosynthetic Pathways By Linking Pmentioning

confidence: 99%

“…The highest perylenequinones production was observed with fructose and the transcription of the putative perylenequinones biosynthetic genes including the Zink-finger transcription factor, and important enzymes in precursor supply were upregulated, whereas competing pathways, such as fatty acid synthesis were downregulated. This could be attributed to the activity of global regulators, e.g., Cre1, PaC (upregulated in presence of fructose) and LaeA (downregulated) (Liu et al, 2020 ). To summarize, even though the whole genome sequence of S .…”

Section: Successful Elucidation Of Biosynthetic Pathways By Linking Pmentioning

confidence: 99%

Current State and Future Directions of Genetics and Genomics of Endophytic Fungi for Bioprospecting Efforts

Sagita

Quax

Haslinger

2021

Front. Bioeng. Biotechnol.

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The bioprospecting of secondary metabolites from endophytic fungi received great attention in the 1990s and 2000s, when the controversy around taxol production from Taxus spp. endophytes was at its height. Since then, hundreds of reports have described the isolation and characterization of putative secondary metabolites from endophytic fungi. However, only very few studies also report the genetic basis for these phenotypic observations. With low sequencing cost and fast sample turnaround, genetics- and genomics-based approaches have risen to become comprehensive approaches to study natural products from a wide-range of organisms, especially to elucidate underlying biosynthetic pathways. However, in the field of fungal endophyte biology, elucidation of biosynthetic pathways is still a major challenge. As a relatively poorly investigated group of microorganisms, even in the light of recent efforts to sequence more fungal genomes, such as the 1000 Fungal Genomes Project at the Joint Genome Institute (JGI), the basis for bioprospecting of enzymes and pathways from endophytic fungi is still rather slim. In this review we want to discuss the current approaches and tools used to associate phenotype and genotype to elucidate biosynthetic pathways of secondary metabolites in endophytic fungi through the lens of bioprospecting. This review will point out the reported successes and shortcomings, and discuss future directions in sampling, and genetics and genomics of endophytic fungi. Identifying responsible biosynthetic genes for the numerous secondary metabolites isolated from endophytic fungi opens the opportunity to explore the genetic potential of producer strains to discover novel secondary metabolites and enhance secondary metabolite production by metabolic engineering resulting in novel and more affordable medicines and food additives.

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L-Arginine enhanced perylenequinone production in the endophytic fungus Shiraia sp. Slf14(w) via NO signaling pathway

Chen

Yang

Liu

et al. 2022

Appl Microbiol Biotechnol

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Transcriptome analysis on fructose as the sole carbon source enhancing perylenequinones production of endophytic fungus Shiraia sp. Slf14

Cited by 7 publications

References 50 publications

Heat stress-induced NO enhanced perylenequinone biosynthesis of Shiraia sp. via calcium signaling pathway

Heat stress-induced NO enhanced perylenequinone biosynthesis of Shiraia sp. via calcium signaling pathway

Current State and Future Directions of Genetics and Genomics of Endophytic Fungi for Bioprospecting Efforts

L-Arginine enhanced perylenequinone production in the endophytic fungus Shiraia sp. Slf14(w) via NO signaling pathway

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