Transcriptome analysis reveals differential gene expression in intramuscular adipose tissues of Jinhua and Landrace pigs

Abstract

Meat is a rich source of protein, fatty acids and carbohydrates for human needs. In addition to necessary nutrients, high fat contents in pork increase the tenderness and juiciness of the meat, featuring diverse application in various dishes. This study investigated the transcriptomic profiles of intramuscular adipose tissues in Jinhua and Landrace pigs by employing advanced RNA sequencing. Results showed significant interesting to note that there were significant differences in the expression of genes. 1,632 genes showed significant differential expression, 837 genes were up-regulated and 195 genes were down-regulated. Variations in genes responsible for cell aggregation, extracellular matrix formation, cellular lipid catabolic process, and fatty acid binding strongly supported that both pig breeds feature variable fat and muscle metabolism. Certain differentially expressed genes are included in the pathway of mitogen-activated protein kinase signaling pathway, Ras signaling pathway and insulin pathway. Results from real-time quantitative polymerase chain reaction also validated the differential expression of 17 mRNAs between meats of the two pig breeds. Overall, these findings reveal significant differences in fat and protein metabolism of intramuscular adipose tissues of two pig breeds at the transcriptomic level and suggest diversification at the genetic level between breeds of the same species.

Adipose gene expression profiles reveal novel insights into the adaptation of northern Eurasian semi-domestic reindeer (Rangifer tarandus)

“…The majority of adipose transcriptome studies using high-throughput RNA sequencing (RNA-Seq) have been limited to mice [20,21], sheep [22][23][24], pigs [25][26][27], and humans [28][29][30]. Adipose transcriptomes are affected by the type of adipose, as well as by the sex and age of the animal [31].…”

Distinct Patterns of PPARγ Promoter Usage, Lipid Degradation Activity, and Gene Expression in Subcutaneous Adipose Tissue of Lean and Obese Swine

“…Current gene annotation based on pig genome build 10.2 is composed of gene identification results from expressed sequence cDNA/tag sequences, bioinformatics prediction, and RNA-seq analyses. Several studies have carried out RNA-seq experiments to profile the transcriptomes of pigs [38,39,40,41]. The present study identified 24,718 expressed transcription units in the subcutaneous adipose tissue of lean (DL) and obese (Min) pig breeds at two different developmental stages (juvenile, 4 weeks old; juvenile-to-adult, 16 weeks old).…”

Whole Transcriptome Analysis of Mesenchyme Tissue in Sika Deer Antler Revealed the CeRNAs Regulatory Network Associated With Antler Development

“…CeRNET plays a widely regulatory role in tissue and organ development (Xu et al, 2016;Li et al, 2017;Liu et al, 2018;Miao et al, 2018). To interpret the interplay among diverse RNAs (mRNA, miRNA, and lncRNA) during the antler development, ceRNET was constructed for the first time.…”