Transcriptome analysis reveals key genes and pathways associated with egg production in Nandan-Yao domestic chicken

Sun, Tiantian; Cong, Xiao; Deng, Jixian; Yang, Zhuliang; Zou, Leqin; Du, Wenya; Li, Shuxia; Huo, Xianqiang; Zeng, Lei; Yang, Xiurong

doi:10.1016/j.cbd.2021.100889

Cited by 16 publications

(13 citation statements)

References 44 publications

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“…Zhang et al [ 22 ] found the pathway correlated with eggshell quality in chickens. The high DEGs in the “cytokine–cytokine receptor interaction” in this study coincided with reports on egg production in Nandan-Yao domestic chicken and Muscovy duck [ 23 , 24 ]. In brief, the three pathways, “neuroactive ligand–receptor interaction”, “calcium signaling pathway”, and “cytokine–cytokine receptor interaction” are considered to be closely related to chicken egg production performance.…”

Section: Discussionsupporting

confidence: 89%

Transcriptomics and Metabolomics Analysis of the Ovaries of High and Low Egg Production Chickens

Huang

Zhang

Cao

et al. 2022

Animals

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Egg production is a pivotal indicator for evaluating the fertility of poultry, and the ovary is an essential organ for egg production and plays an indispensable role in poultry production and reproduction. In order to investigate different aspects of egg production mechanisms in different poultry, in this study we performed a metabolomic analysis of the transcriptomic combination of the ovaries of two chicken breeds, the high-production Ninghai indigenous chickens and the low-production Wuliangshan black-boned chickens, to analyze the biosynthesis and potential key genes and metabolic pathways in the ovaries during egg production. We predicted four genes in the transcriptomic that are associated with egg production, namely P2RX1, INHBB, VIPR2, and FABP3, and identified three important pathways during egg production, “Calcium signaling pathway”, “Neuroactive ligand–receptor interaction” and “Cytokine–cytokine receptor interaction”, respectively. In the metabolomic 149 significantly differential metabolites were identified, 99 in the negative model and 50 in the positive model, of which 17α-hydroxyprogesterone, iloprost, spermidine, and adenosine are important metabolites involved in reproduction. By integrating transcriptomics and metabolomics, the correlation between specific differential genes and differential metabolites identified important gene-metabolite pairs “VIPR2-Spermidine” and “P2RX1-Spermidine” in egg production. In conclusion, these data provide a better understanding of the molecular differences between the ovaries of low- and high-production hens and provide a theoretical basis for further studies on the mechanics of poultry egg production.

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Section: Discussionsupporting

confidence: 89%

Transcriptomics and Metabolomics Analysis of the Ovaries of High and Low Egg Production Chickens

Huang

Zhang

Cao

et al. 2022

Animals

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“…In duck's ( 6 ), goose's ( 21 ), and goat's ( 22 ) ovary transcriptome studies, the actin cytoskeleton has been discovered to be related to ovary function. The phagosome and cell adhesion molecules (CAMs) were consistently reported in a study comparing the ovary of high- and low-laying ducks ( 23 ). Overall, these signaling pathways may control ovary development and may be involved in the egg production of Gaoyou duck.…”

Section: Discussionmentioning

confidence: 81%

Identification of differentially expressed genes and signaling pathways in Gaoyou duck ovary at different physiological stages

et al. 2023

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IntroductionGaoyou duck is famous in China and abroad for its good production of double-yolk eggs. However, there has been no systematic research on the egg-laying characteristics of the Gaoyou duck, which limits the development and utilization of breed resource.MethodsTo identify the essential genes related to ovarian development, the transcriptome profiles of the ovaries of Gaoyou ducks at different physiological stages were analyzed. The transcriptome profiles of the ovaries of Gaoyou ducks at 150 d (before laying), 240 d (egg laying) and 500 d (nesting) were constructed, and the differentially expressed genes (DEGs) underwent GO (gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses.ResultsThe 6 randomly selected DEGs were verified by real-time fluorescent quantitative PCR that their relative expression was consistent with the transcriptional expression profile. Furthermore, KEGG analysis found that 8 candidate signaling pathways were essential for ovarian development, including the MAPK signaling pathway, Progesterone-mediated oocyte maturation, Cell adhesion molecules (CAMs), NOD-like receptor signaling pathway, ECM-receptor interaction, Focal adhesion, TGF-beta signaling path-way and Phagosome. Finally, 5 key DEGs were identified to participate in ovarian development, including TGIF1, TGFBR2, RAF1, PTK2 and FGF10.DiscussionOur findings reveal the mechanisms under-lying the molecular regulation of related genes in Gaoyou duck ovarian development.

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“…In the present study, most DEGs and DELs possessed nonadditivity pattern in hybrids, indicating nonadditivity was the critical gene expression pattern affecting traits heterosis. Functional enrichment analysis showed that nonadditive genes were associated with animal organ development, female gonad development, ECM-receptor interaction and GnRH signaling pathway, such as laminin subunit alpha 4 (VTN), CD36 molecule (CD36) and fibronectin 1 (FN1), which were revealed to function in ovary cell growth and proliferation (49)(50)(51) and specifically expressed in crossbreeds' ovary. In addition, the nonadditive gene purinergic receptor P2X 1 (P2RX1) was identified, which was enriched in calcium signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Genetic Basis of Sexual Maturation Heterosis: Insights From Ovary lncRNA and mRNA Repertoire in Chicken

et al. 2022

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Sexual maturation is fundamental to the reproduction and production performance, heterosis of which has been widely used in animal crossbreeding. However, the underlying mechanism have long remained elusive, despite its profound biological and agricultural significance. In the current study, the reciprocal crossing between White Leghorns and Beijing You chickens were performed to measure the sexual maturation heterosis, and the ovary lncRNAs and mRNAs of purebreds and crossbreeds were profiled to illustrate molecular mechanism of heterosis. Heterosis larger than 20% was found for pubic space and oviduct length, whereas age at first egg showed negative heterosis in both crossbreeds. We identified 1170 known lncRNAs and 1994 putative lncRNAs in chicken ovary using a stringent pipeline. Gene expression pattern showed that nonadditivity was predominant, and the proportion of nonadditive lncRNAs and genes was similar between two crossbreeds, ranging from 44.24% to 49.15%. A total of 200 lncRNAs and 682 genes were shared by two crossbreeds, respectively. GO and KEGG analysis showed that the common genes were significantly enriched in the cell cycle, animal organ development, gonad development, ECM-receptor interaction, calcium signaling pathway and GnRH signaling pathway. Weighted gene co-expression network analysis (WGCNA) identified that 7 out of 20 co-expressed lncRNA-mRNA modules significantly correlated with oviduct length and pubic space. Interestingly, genes harbored in seven modules were also enriched in the similar biological process and pathways, in which nonadditive lncRNAs, such as MSTRG.17017.1 and MSTRG.6475.20, were strongly associated with nonadditive genes, such as CACNA1C and TGFB1 to affect gonad development and GnRH signaling pathway, respectively. Moreover, the results of real-time quantitative PCR (RT-qPCR) correlated well with the transcriptome data. Integrated with positive heterosis of serum GnRH and melatonin content detected in crossbreeds, we speculated that nonadditive genes involved in the GnRH signaling pathway elevated the gonad development, leading to the sexual maturation heterosis. We characterized a systematic landscape of ovary lncRNAs and mRNAs related to sexual maturation heterosis in chicken. The quantitative exploration of hybrid transcriptome changes lays foundation for genetic improvement of sexual maturation traits and provides insights into endocrine control of sexual maturation.

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Transcriptome analysis reveals key genes and pathways associated with egg production in Nandan-Yao domestic chicken

Cited by 16 publications

References 44 publications

Transcriptomics and Metabolomics Analysis of the Ovaries of High and Low Egg Production Chickens

Transcriptomics and Metabolomics Analysis of the Ovaries of High and Low Egg Production Chickens

Identification of differentially expressed genes and signaling pathways in Gaoyou duck ovary at different physiological stages

Genetic Basis of Sexual Maturation Heterosis: Insights From Ovary lncRNA and mRNA Repertoire in Chicken

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