Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus Magnaporthe oryzae

Abstract

(http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Magnaporthe oryzae appressonium formulation <p>Analysis of genome-wide gene-expression changes during spore germination and appressorium formation in <it>Magnaporthe oryzae</it> revealed that protein degradation and amino-acid metabolism are essential for appressorium formation and subsequent infection.</p> Abstract Background: Rice blast disease is caused by the filamentous Ascomycetous fungus Magnaporthe oryzae and results in significant annual rice yield losses worldwide. Infection by this and many other fungal plant pathogens requires the development of a specialized infection cell called an appressorium. The molecular processes regulating appressorium formation are incompletely understood.

Potential for using crude extract of Sarocladium oryzae for suppression of rice blast

“…Cerulenin is previously known to be a potent inhibitor of enzymes involved in fatty acid synthesis in a range of microorganisms (Omura, 1976). This group of enzymes is responsible for the production of lipids, which are vital components of cell plasma membrane (Campbell & Cronan, 2001;Oh et al, 2008). The damage caused by cerulenin to plasma membrane could be detected indirectly by staining the fungus cell wall structures using Calcofluor White reagent under fluorescent optical microscopy.…”

Cloning and Expression of the Pathogenicity-Related Genes of Oidium heveae in the Infection Process

“…DGE method uses data of relative tag frequencies to show gene expression differences [13]. Transcriptomic analysis has also been used to study pathogenicity-related genes [5]. Yeonyee Oh et al [5] reported the first comprehensive genome-wide transcript profile and the functional analysis of infection structure formation of Magnaporthe oryzae.…”

“…Transcriptomic analysis has also been used to study pathogenicity-related genes [5]. Yeonyee Oh et al [5] reported the first comprehensive genome-wide transcript profile and the functional analysis of infection structure formation of Magnaporthe oryzae. Gene knockout and complementary strategies have also been used to study the function of pathogenicity-related genes [14].…”

“…belong to three categories, the metabolism-and growth-related genes, and genes encoding hypothetical proteins [5]. The expression of these genes during different infection stages was further analyzed by qRT-PCR and compared to the transcriptomic data.…”

“…Presently, the main disease control measures rely on new pesticides and resistant varieties [3]. In China, the studies of O. heveae have been limited to the cytology observation on the interaction of O. heveae with rubber tree, including the infection and development process and environmental factors such as temperature [4,5]. O. heveae is an obligate parasite, it cannot be cultured on medium [6].…”