Transcriptome Analysis Reveals the Age-Related Developmental Dynamics Pattern of the Longissimus Dorsi Muscle in Ningxiang Pigs

Abstract

The growth and development of the Longissimus Dorsi muscle are complex, playing an important role in the determination of pork quality. The study of the Longissimus Dorsi muscle at the mRNA level is particularly crucial for finding molecular approaches to improving meat quality in pig breeding. The current study utilized transcriptome technology to explore the regulatory mechanisms of muscle growth and intramuscular fat (IMF) deposition in the Longissimus Dorsi muscle at three core developmental stages (natal stage on day 1, growing stage on day 60, and finishing stage on day 210) in Ningxiang pigs. Our results revealed 441 differentially expressed genes (DEGs) in common for day 1 vs. day 60 and day 60 vs. day 210, and GO (Gene Ontology) analysis showed that candidate genes RIPOR2, MEGF10, KLHL40, PLEC, TBX3, FBP2, and HOMER1 may be closely related to muscle growth and development, while KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that DEGs (UBC, SLC27A5, RXRG, PRKCQ, PRKAG2, PPARGC1A, PLIN5, PLIN4, IRS2, and CPT1B) involved the PPAR (Peroxisome Proliferator-Activated Receptor) signaling pathway and adipocytokine signaling pathway, which might play a pivotal role in the regulation of IMF deposition. PPI (Protein-Protein Interaction Networks) analysis found that the STAT1 gene was the top hub gene. Taken together, our results provide evidence for the molecular mechanisms of growth and development and IMF deposition in Longissimus Dorsi muscle to optimize carcass mass.

Muscle Transcriptome Analysis of Mink at Different Growth Stages Using RNA-Seq

“…In the study, 51 DEGs associated with muscle development were identified during the growth and development stages of mink. Notably, IGF2, MYOG, MEGF10, MYMX, MYMK, SOX8, and PITX1 exhibited upregulated expression at 45 days, with stronger expression in skeletal muscle satellite cells where they play crucial roles in muscle regeneration [7,27,28]. Furthermore, it has been observed that certain genes exhibit a requirement for cooperative interactions in order to ensure their proper functionality.…”

“…Thus, studying the genetic regulatory mechanisms of mink growth is crucial for the mink breeding industry. In recent years, with the rapid development of sequencing technology, genomics has been widely used in the study of molecular regulation mechanisms of animal growth and development, promoting the process of animal genome selection breeding [3][4][5][6][7]. However, the relevant research on mink is still lagging behind.…”

Single-nucleus RNA sequencing and lipidomics reveal characteristics of transcriptional and lipid composition in porcine longissimus dorsi muscle

“…PPARGC1A plays a crucial role in reducing lipid deposition [ 53 ]. UBC , SLC27A5 , RXRG , PRKCQ , PRKAG2 , PPARGC1A , PLIN5 , PLIN4 , IRS2 , and CPT1B play a role in regulating the growth and development of the longissimus dorsi muscle in Ningxiang pigs [ 54 ]. The genes PPARA , PPARG , SREBF1 , and PPARGC1A genes are involved in regulating fat deposition in the longissimus dorsi muscle of pigs.…”

Detection of Runs of Homozygosity and Identification of Candidate Genes in the Whole Genome of Tunchang Pigs

“…Furthermore, we detected candidate genes related to meat quality traits in Tunchang pigs. The GYS1 gene is involved in skeletal muscle glycogen synthesis [60], the PHLPP1 gene may be involved in bone development [61], and the SLC27A5 gene may play a key role in intramuscular fat deposition [62]. Additionally, the CRTC1 gene, which was enriched in our Tunchang pig population, has been reported to regulate fat deposition and indirectly affect meat quality traits [63].…”