Transcriptome analysis reveals the molecular mechanisms of the novel Lactobacillus pentosus pentocin against Bacillus cereus

Xu, Zhi-Qiang; Yang, Qingli; Zhu, Yinglian

doi:10.1016/j.foodres.2021.110840

Cited by 21 publications

(4 citation statements)

References 75 publications

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“…After adding H 3 BO 3 , NaBO 2 , NaBO 3 , Na 2 B 4 O 7 , K 2 B 4 O 7 , and (NH 4 ) 2 B 4 O 7 , AI-2 activity increased by 33.44%, 38.28%, 29.58%, 25.01%, 16.95%, and 21.16%, respectively. AI-2 was derived from precursor 4,5-dihydroxy-2,3-pentanedione (DPD) [ 20 ]. DPD was reported to be quite unstable toward rearrangement and oligomerization, but it can exist as a balanced mixture of borate complexes and generate active AI-2 [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…The expression level of aphA was significantly downregulated at high concentrations from 0.8 mM to 1.0 mM H 3 BO 3 treatment ( p < 0.05). The luxS gene encodes S-ribosylhomocysteinase, an enzyme which generates the autoinducer-2 (AI-2) precursor of DPD from S-ribosyl-L -homocysteine [ 20 ]. When AI-2 enters cells, it firstly binds to the AI-2 transports system substrate-binding protein encoded by lsrB .…”

Section: Resultsmentioning

confidence: 99%

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Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Chen

Yan

Zhan

et al. 2022

IJMS

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Boron is an essential element for autoinducer-2 (AI-2) synthesis of quorum sensing (QS) system, which affects bacterial collective behavior. As a living biocatalyst, biofilms can stably catalyze the activity of intracellular enzymes. However, it is unclear how boron affects biofilm formation in E. coli, particularly recombinant E. coli with intracellular enzymes. This study screened different boron derivatives to explore their effect on biofilm formation. The stress response of biofilm formation to boron was illuminated by analyzing AI-2 activity, extracellular polymeric substances (EPS) composition, gene expression levels, etc. Results showed that boron derivatives promote AI-2 activity in QS system. After treatment with H3BO3 (0.6 mM), the AI-2 activity increased by 65.99%, while boron derivatives increased the biomass biofilms in the order H3BO3 > NaBO2 > Na2B4O7 > NaBO3. Moreover, treatment with H3BO3 (0.6 mM) increased biomass by 88.54%. Meanwhile, AI-2 activity had a linear correlation with polysaccharides and protein of EPS at 0–0.6 mM H3BO3 and NaBO2 (R2 > 0.8). Furthermore, H3BO3 upregulated the expression levels of biofilm formation genes, quorum sensing genes, and flagellar movement genes. These findings demonstrated that boron promoted biofilm formation by upregulating the expression levels of biofilm-related genes, improving the QS system AI-2 activity, and increasing EPS secretion in E. coli.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Chen

Yan

Zhan

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…In addition, functional enrichment analyses, including gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), were performed to identify DEGs that were significantly enriched in GO terms and metabolic pathways. A Bonferroni-corrected P < 0.05 was considered statistically significant …”

Section: Methodsmentioning

confidence: 99%

Unraveling the Genetic Adaptations in Cell Surface Composition and Transporters of Lactiplantibacillus plantarum for Enhanced Acid Tolerance

Zhu,

Sun,

Zhang

et al. 2024

J. Agric. Food Chem.

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This study employed adaptive laboratory evolution to improve the acid tolerance of Lactiplantibacillus plantarum, a vital strain in food fermentation and a potential probiotic. Phenotype and genomic analyses identified the overexpression of stress response proteins, ATP synthases, and transporters as pivotal in conferring acid tolerance to the evolved strains. These adaptations led to a shorter lag phase, improved survival rates, and higher intracellular pH values compared to the wild-type strain under acid stress conditions. Additionally, the evolved strains showed an increased expression of genes in the fatty acid synthesis pathway, resulting in a higher production of unsaturated fatty acids. The changes in cell membrane composition possibly prevented H + influx, while mutant genes related to cell surface structure contributed to observed elongated cells and thicker cell surface. These alterations in cell wall and membrane composition, along with improved transporter efficiency, were key factors contributing to the enhanced acid tolerance in the evolved strains.

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“…Z. Xu, Yang, et al. (2021) also reported that pentocin produced by Lactobacillus pentosus inhibited the phosphorylation and autoinducer‐2 imported by downregulating the expression levels of LuxS and LsrB genes in QS signal pathway, which suppressed biofilm formation of Bacillus cereus .…”

Section: Antibiofilm Mechanisms Of Lab Bacteriocinsmentioning

confidence: 98%

Combating biofilms of foodborne pathogens with bacteriocins by lactic acid bacteria in the food industry

Pang

Song

Chen

et al. 2022

Comp Rev Food Sci Food Safe

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Most foodborne pathogens have biofilm‐forming capacity and prefer to grow in the form of biofilms. Presence of biofilms on food contact surfaces can lead to persistence of pathogens and the recurrent cross‐contamination of food products, resulting in serious problems associated with food safety and economic losses. Resistance of biofilm cells to conventional sanitizers urges the development of natural alternatives to effectively inhibit biofilm formation and eradicate preformed biofilms. Lactic acid bacteria (LAB) produce bacteriocins which are ribosomally synthesized antimicrobial peptides, providing a great source of nature antimicrobials with the advantages of green and safe properties. Studies on biofilm control by newly identified bacteriocins are increasing, targeting primarily onListeria monocytogenes, Staphylococcus aureus, Salmonella, and Escherichia coli. This review systematically complies and assesses the antibiofilm property of LAB bacteriocins in controlling foodborne bacterial‐biofilms on food contact surfaces. The bacteriocin‐producing LAB genera/species, test method (inhibition and eradication), activity spectrum and surfaces are discussed, and the antibiofilm mechanisms are also argued. The findings indicate that bacteriocins can effectively inhibit biofilm formation in a dose‐dependent manner, but are difficult to disrupt preformed biofilms. Synergistic combination with other antimicrobials, incorporation in nanoconjugates and implementation of bioengineering can help to strengthen their antibiofilm activity. This review provides an overview of the potential and application of LAB bacteriocins in combating bacterial biofilms in food processing environments, assisting in the development and widespread use of bacteriocin as a promising antibiofilm‐agent in food industries.

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Transcriptome analysis reveals the molecular mechanisms of the novel Lactobacillus pentosus pentocin against Bacillus cereus

Cited by 21 publications

References 75 publications

Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Boron Derivatives Accelerate Biofilm Formation of Recombinant Escherichia coli via Increasing Quorum Sensing System Autoinducer-2 Activity

Unraveling the Genetic Adaptations in Cell Surface Composition and Transporters of Lactiplantibacillus plantarum for Enhanced Acid Tolerance

Combating biofilms of foodborne pathogens with bacteriocins by lactic acid bacteria in the food industry

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