Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily

He, Ling; Wu, Ze; Teng, Nianjun

doi:10.3390/ijms222212124

Cited by 9 publications

(12 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The total number of metabolites in plants is as high as 200,000, making them an ideal target for studying biosynthesis regulation [ 68 ]. In recent years, the genetic mechanism of plant metabolite changes has been studied [ 69 , 70 ]. We detected 650 metabolites that could be annotated in MS2 database using the extensive targeted metabolomics method, and the number of detected metabolites was higher than that of previous studies on frost resistance of wheat [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

Dong

Liu

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

Background Low temperature is a crucial stress factor of wheat (Triticum aestivum L.) and adversely impacts on plant growth and grain yield. Multi-million tons of grain production are lost annually because crops lack the resistance to survive in winter. Particularlly, winter wheat yields was severely damaged under extreme cold conditions. However, studies about the transcriptional and metabolic mechanisms underlying cold stresses in wheat are limited so far. Results In this study, 14,466 differentially expressed genes (DEGs) were obtained between wild-type and cold-sensitive mutants, of which 5278 DEGs were acquired after cold treatment. 88 differential accumulated metabolites (DAMs) were detected, including P-coumaroyl putrescine of alkaloids, D-proline betaine of mino acids and derivativ, Chlorogenic acid of the Phenolic acids. The comprehensive analysis of metabolomics and transcriptome showed that the cold resistance of wheat was closely related to 13 metabolites and 14 key enzymes in the flavonol biosynthesis pathway. The 7 enhanced energy metabolites and 8 up-regulation key enzymes were also compactly involved in the sucrose and amino acid biosynthesis pathway. Moreover, quantitative real-time PCR (qRT-PCR) revealed that twelve key genes were differentially expressed under cold, indicating that candidate genes POD, Tacr7, UGTs, and GSTU6 which were related to cold resistance of wheat. Conclusions In this study, we obtained the differentially expressed genes and differential accumulated metabolites in wheat under cold stress. Using the DEGs and DAMs, we plotted regulatory pathway maps of the flavonol biosynthesis pathway, sucrose and amino acid biosynthesis pathway related to cold resistance of wheat. It was found that candidate genes POD, Tacr7, UGTs and GSTU6 are related to cold resistance of wheat. This study provided valuable molecular information and new genetic engineering clues for the further study on plant resistance to cold stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

Dong

Liu

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…Omics technology provides a unique opportunity to study the regulatory mechanism of complex networks related to growth promotion [19]. With the rapid development of sequencing tools, transcriptome sequencing technology has been widely used to elucidate gene function through highthroughput analysis of genomic information, providing a powerful opportunity to systematically study the relationship between genotype and phenotype in transcriptional regulation [20]. For example, it is widely used to study tobacco disease resistance and growth promotion to reveal differentially expressed genes in various biological processes.…”

Section: Introductionmentioning

confidence: 99%

“…For example, it is widely used to study tobacco disease resistance and growth promotion to reveal differentially expressed genes in various biological processes. However, low abundance and unknown transcripts cannot be distinguished [20]. Compared to traditional chemical analysis, the use of liquid chromatography/mass spectrometry (LC/MS) promotes metabolomics by detecting a large number of compounds [21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomics and transcriptomics combined with physiology reveal key metabolic pathway responses in tobacco roots exposed to NaHS

Yang,

Wen,

Yang

et al. 2024

Preprint

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) is a new endogenous gas signaling molecule alongside nitric oxide (NO) and carbon monoxide (CO). In recent years, many studies have shown that it is involved in a variety of physiological activities, promoting root organogenesis, regulating stomatal movement and photosynthesis, and playing an important role in promoting plant growth and development and improving the stress resistance of the plant Plants can play. Tobacco is a special and an important cash crop whose growth usually determines the economic income of farmers. Tobacco root development directly affects leaf growth, disease resistance, chemical composition and yield. However, to date, there are limited studies on the mechanism of H2S in promoting tobacco growth. In this study, tobacco seedlings were exposed to NaHS (exogenous H2S donor) at concentrations of 0, 200, 400, 600, and 800 mg/L. With the gradual increase of NaHS concentration, the root length, wet weight, root activity and antioxidant enzymatic activities of CAT (catalase), SOD (superoxide dismutase) and POD (peroxidase) of tobacco roots showed an increasing tendency. The transcriptome and metabolome showed that NaHS mainly affected 162 key genes, 44 key enzymes, and two metabolic pathways (the brassinolide synthesis pathway and the aspartic acid biosynthesis pathway) in tobacco seedlings after treatment with 600 mg/L NaHS. On the one hand, the addition of exogenous NaHS can promote the development of tobacco roots as the basis of tobacco planting. On the other hand, the use of NaHS can reduce the use of pesticides and better protect the ecological environment. In conclusion, this study provides new insights into the main metabolic pathway in the response of tobacco roots to NaHS and new genetic clues for further investigation- of plant root development.

show abstract

“…Lily (Lilium spp.) is an important ornamental crop for production of cut and potted flowers and for application as groundcover in landscaping [1][2][3][4]. In addition, lily bulbs are an extremely important food and medicinal material in China.…”

Section: Introductionmentioning

confidence: 99%

Starch Degradation and Sucrose Accumulation of Lily Bulbs after Cold Storage

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Functional lilies are a group of edible lily cultivars with great potential for landscape application. Low-temperature storage can significantly improve their taste, but the knowledge of this process is largely unknown. In this study, we used the functional lilies ‘Fly Shaohua’ and ‘Fly Tiancheng’ as materials. Through physiological observation and transcriptome analysis during the bulbs’ cold storage, it was found that the starch degradation and sucrose accumulation in bulbs contributed to taste improvement. After 60 d of cold storage, the sucrose accumulation was highest and the starch content was lower in the bulbs, suggesting this time-point was optimal for consumption. Accompanying the fluctuation of sucrose content during cold storage, the enzyme activities of sucrose phosphate synthase and sucrose synthase for sucrose synthesis were increased. Transcriptome analysis showed that many differentially expressed genes (DEGs) were involved in the starch and sucrose metabolism pathway, which might promote the conversion of starch to sucrose in bulbs. In addition, the DEGs involved in dormancy and stress response were also determined during cold storage, which might explain the decreased sucrose accumulation with extended storage time over 60 d due to the energy consumption for dormancy release. Taken together, our results indicated sucrose accumulation was a main factor in the taste improvement of lily bulbs after cold storage, which is attributable to the different gene expression of starch and sucrose metabolism pathways in this process.

show abstract

Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily

Cited by 9 publications

References 71 publications

Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

Transcription-associated metabolomic profiling reveals the critical role of frost tolerance in wheat

Metabolomics and transcriptomics combined with physiology reveal key metabolic pathway responses in tobacco roots exposed to NaHS

Starch Degradation and Sucrose Accumulation of Lily Bulbs after Cold Storage

Contact Info

Product

Resources

About