Transcriptome and metabonomics combined analysis revealed the energy supply mechanism involved in fruiting body initiation in Chinese cordyceps

Abstract

Chinese cordyceps was one of most valuable traditional Chinese medicine fungi. To elucidate the molecular mechanisms related to energy supply mechanism involved in the initiation and formation of primordium in Chinese cordyceps, we performed the integrated metabolomic and transcriptomic analyses of it at pre-primordium period, primordium germination period and after-primordium period, respectively. Transcriptome analysis showed that many genes related to ‘starch and sucrose metabolism’, ‘fructose and mannose metabolism’, ‘linoleic acid metabolism’, ‘fatty acids degradation’ and ‘glycerophospholipid metabolism’ were highly up-regulated at primordium germination period. Metabolomic analysis showed many metabolites regulated by these genes in these metabolism pathways were also markedly accumulated at this period. Consequently, we inferred that carbohydrate metabolism and β-oxidation pathway of palmitic acid and linoleic acid worked cooperatively to generate enough acyl-CoA, and then entered TCA cycle to provide energy for fruiting body initiation. Overall, our finding provided important information for further exploring the energy metabolic mechanisms of realizing the industrialization of Chinese cordyceps artificial cultivation.

Combined transcriptome and metabolome analysis of sugar and fatty acid of aromatic coconut and non-aromatic coconut in China

“…Exploring the roles of organic acids and sugars as chemical messengers in morphogenesis of plant poses a significant challenge, particularly in understanding their roles and relationships with other cellular communication pathways ( Igamberdiev and Bykova, 2023 , Jawad et al, 2020 ). Transcriptomic technologies have been utilized to elucidate the genes associated with the metabolism of fatty acids and sucrose ( He et al, 2023 , Liu et al, 2020 ). Thus, this study proposes a plausible mechanism for recognizing prominent candidate genes that are accountable for determining sugar and fatty acid content.…”