Transcriptome and proteome analyses to investigate the molecular underpinnings of cold response in the Colorado potato beetle, Leptinotarsa decemlineata

Govaere, Louise; Morin, Mathieu D.; Frigault, Jacques J.; Boquel, Sébastien; Cohen, Alejandro; Lamarre, Simon G.; Morin, Pier Jr

doi:10.1016/j.cryobiol.2019.03.011

Cited by 16 publications

(11 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diapause, an adaptive strategy to endure a cold winter, is widespread among insects. 21,24,[46][47][48][49] 'Multi-omics' approaches have been applied to decipher the molecular underpinnings of diapause in some insect species, including the Tetranychus urticae, 29 Laodelphax striatellus, 30 and Aphidius gifuensis Ashmaed. 50 Up-regulated genes in diapause-destined insects were closely related to lipid metabolism, energy production and conversion, carbohydrate metabolism and transport, and molecular chaperones.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and proteomic analyses of the mechanisms of overwintering diapause in soybean pod borer (Leguminivora glycinivorella)

Yang

Wang

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Soybean pod borer (Leguminivora glycinivorella) is an important soybean pest in northeastern Asia, whose mature larvae overwinter in a diapause state. Disruption of winter diapause may be a valuable tool in pest management. However, the molecular mechanisms regulating diapause in this species have not yet been elucidated. RESULTS: We compared the transcriptomes and proteomes between diapause and mature larvae and between mature and newly developed pupae to identify the genes and proteins associated with diapause. Thirty-seven differentially expressed genes and their proteins changed synchronously between diapause and mature larvae and 82 changed synchronously between diapause larvae and newly developed pupae. Among these, genes involved in fatty acid biosynthesis and the longevity regulating pathway were up-regulated in diapause larvae and down-regulated in newly developed pupae, suggesting that they may regulate diapause. One fatty acid synthase (FAS) gene and two small heat shock genes (HSP19.8 and HSP18.9) were chosen for further functional analysis. After RNA interference (RNAi)-mediated knockdown of FAS, the survival of mature larvae was significantly lower than that of control larvae, but the mean developmental time from first-instar larva to adult remained unchanged. RNAi-mediated knockdown of HSP19.8 and HSP18.9 severely shortened the mean developmental time, causing approximately 50% larvae to develop directly into pupae. CONCLUSION: FAS and the small heat shock gene play roles in diapause regulation and larvae survival. This study provides important information that may assist in understanding the molecular regulatory mechanisms of overwintering diapause of this important agricultural insect pest.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptomic and proteomic analyses of the mechanisms of overwintering diapause in soybean pod borer (Leguminivora glycinivorella)

Yang

Wang

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…Some studies have focused on insect resistance to insecticides [28][29][30][31]. Other studies have examined insect adaptability to extreme environments [32,33], or focus on selected areas of the genome such as chemosensory genes [34]. There are few studies on insect diapause using RNA-Seq, but Hao et al (2019) identified the candidate genes (rai1 and foxo) related to the FOXO pathway in the egg diapause regulation of Locusta migratoria [35].…”

Section: Introductionmentioning

confidence: 99%

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae)

Zhang

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background The parasitoid wasp, Trichogramma dendrolimi, can enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi. Previous studies on diapause have mainly focused on ecological characteristics, so the molecular basis of diapause in T. dendrolimi is unknown. We compared transcriptomes of diapause and non-diapause T. dendrolimi to identify key genes and pathways involved in diapause development. Results Transcriptome sequencing was performed on diapause prepupae, pupae after diapause, non-diapause prepupae, and pupae. Analysis yielded a total of 87,022 transcripts with an average length of 1604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7593 sequences with an average length of 3351 bp. Among them, 5702 genes were differentially expressed. The result of Gene Ontology (GO) enrichment analysis revealed that regulation of transcription, DNA-templated, oxidation-reduction process, and signal transduction were significantly affected. Ten genes were selected for validation using quantitative real-time PCR (qPCR). The changes showed the same trend as between the qPCR and RNA-Seq results. Several genes were identified as involved in diapause, including ribosomal proteins, zinc finger proteins, homeobox proteins, forkhead box proteins, UDP-glucuronosyltransferase, Glutathione-S-transferase, p53, and DNA damage-regulated gene 1 (pdrg1). Genes related to lipid metabolism were also included. Conclusions We generated a large amount of transcriptome data from T. dendrolimi, providing a resource for future gene function research. The diapause-related genes identified help reveal the molecular mechanisms of diapause, in T. dendrolimi, and other insect species.

show abstract

“…In fact, many studies have used RNAsequencing (RNA-Seq) to solve a wide variety of problems. Some studies focused on insect resistance to insecticide [30][31][32][33] , some on insect adaptability to extreme environments [34,35] , and others on selected genes, like chemosensory genes [36] . But there have been few studies on insect diapause using RNA-Seq.…”

Section: Introductionmentioning

confidence: 99%

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

Zhang¹,

Du²,

Zhang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The parasitoid insect Trichogramma dendrolimi can successfully enter diapause at the prepupal stage. Thus, diapause is an efficient preservation method during the mass production of T. dendrolimi. Previous studies on diapause have focused mostly on ecological characteristics, so the molecular mechanism of diapause in T. dendrolimi is mostly unknown. In this study, we compared transcriptomes of diapause and non-diapause T. dendrolimi to identify genes involved in the development of diapause. Results Transcriptome sequencing was performed using different samples, including diapause prepupae, pupae after diapause, normal prepupae, and pupae. This initially yielded a total of 87,022 unigenes with an average length of 1,604 bp. By removing redundant sequences and those without significant BLAST hits, a non-redundant dataset was generated, containing 7,593 sequences with an average length of 3351 bp. Among the differentially expressed genes, 1,204 were specifically expressed during the diapause stage, and 820 were significantly up-regulated. The result of GO enrichment analysis revealed that binding, oxidation−reduction process, and ribosome biogenesis were significantly affected. Ten genes were selected for validation using a quantitative real-time PCR (qPCR). The changes showed the same trend between the qPCR and RNA-Seq results. Based on previous studies and our data, several genes were identified to be involved in diapause, including UDP-glucuronosyltransferase; Glutathione-S-transferase, ATGs, zinc finger protein genes, cell cycle-related genes, Trehalase, trehalose transporter, hsp68, p53, and DNA damage-regulated gene 1 (pdrg1), and genes related to lipid metabolism were also included. Conclusions In this study, we generated a great amount of transcriptome data from T. dendrolimi, providing a resource for gene function research. The diapause-related genes that we identified establish a valuable basis for future studies on the molecular mechanisms of diapause, not only for T. dendrolimi, but for other species as well.

show abstract

Transcriptome and proteome analyses to investigate the molecular underpinnings of cold response in the Colorado potato beetle, Leptinotarsa decemlineata

Cited by 16 publications

References 58 publications

Transcriptomic and proteomic analyses of the mechanisms of overwintering diapause in soybean pod borer (Leguminivora glycinivorella)

Transcriptomic and proteomic analyses of the mechanisms of overwintering diapause in soybean pod borer (Leguminivora glycinivorella)

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae)

High-throughput profiling of diapause regulated genes from Trichogramma dendrolimi, an important egg parasitoid

Contact Info

Product

Resources

About