Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis

Liu, Huan; Guo, Lei; Zhang, Weiwei; Peng, Jiahui; Chen, Qinna; Cao, Fang; Zhang, Zhaohui; Guo, Mingxiao; Zhang, Han; Mu, Shumei; Kang, Xianjiang

doi:10.1016/j.aqrep.2022.101392

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…2 During the breeding process, it is commonly observed that approximately 15−30% of the crabs undergo precocious puberty and terminal molt, resulting in stagnation of body mass and higher mortality, which seriously affects production and economic benefits. 3 In addition to the influence of external conditions, 4,5 the intrinsic causes also have great effects on the development of individuals. As a carrier of properties of heredity, the male parent sperm is one of the determinants for offspring traits.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with normal E. sinensis, smaller spermatogonia, spermatocytes, and spermatozoa diameters of precocious crabs indicate the occurrence of physiological disturbance. 3 Therefore, understanding the regulatory mechanism of spermatogenesis and fertilization is not only helpful for the evaluation of germ cell quality but also provides important references for breeding screening and germplasm resource protection. Currently, most works mainly focus on the molecular mechanisms of spermatogenesis 6−9 and an acrosome reaction 10−13 in crustaceans, whereas there is limited information on sperm capacitation.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Proteomics Elucidates the Potential Mechanism of Sperm Capacitation of Chinese Mitten Crabs (Eriocheir sinensis)

Cui,

Cai,

Zhang

et al. 2024

J. Proteome Res.

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Sperm capacitation is broadly defined as a suite of biochemical and biophysical changes resulting from the acquisition of fertilization ability. To gain insights into the regulation mechanism of crustacean sperm capacitation, 4D label-free quantitative proteomics was first applied to analyze the changes of sperm in Eriocheir sinensis under three sequential physiological conditions: seminal vesicles (X2), hatched with the seminal receptacle content (X3), and incubated with egg water (X5). In total, 1536 proteins were identified, among which 880 proteins were quantified, with 82 and 224 proteins significantly altered after incubation with the seminal receptacle contents and egg water. Most differentially expressed proteins were attributed to biological processes by Gene Ontology annotation analysis. As the fundamental bioenergetic metabolism of sperm, the oxidative phosphorylation, glycolysis, and the pentose phosphate pathway presented significant changes under the treatment of seminal receptacle contents, indicating intensive regulation for sperm in the seminal receptacle. Additionally, the seminal receptacle contents also significantly increased the oxidation level of sperm, whereas the enhancement of abundance in superoxide dismutase, peroxiredoxin 1, and glutathione S-transferase after incubation with egg water significantly improved the resistance against oxidation. These results provided a new perspective for reproduction studies in crustaceans.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Proteomics Elucidates the Potential Mechanism of Sperm Capacitation of Chinese Mitten Crabs (Eriocheir sinensis)

Cui,

Cai,

Zhang

et al. 2024

J. Proteome Res.

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Sperm acrosomal released proteome reveals MDH and VDAC3 from mitochondria are involved in acrosome formation during spermatogenesis in Eriocheir sinensis

Li,

Yu,

Liu

et al. 2023

Gene

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Integrate Analysis of Eyestalk Proteome and Metabolome in Precocious and Formal Juvenile Female Eriocheir sinensis

Pan,

Yang,

et al. 2024

Fishes

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The Chinese mitten crab (Eriocheir sinensis) is an economically important crustacean. With the development of the E. sisnensis industry, precocity has become a significant challenge in juvenile crab culturing. In this study, the eyestalks of female E. sinensis from precocious (PE) and normal juvenile (NE) groups were used for proteome and metabolome analyses. In total, 731 up-regulated and 657 down-regulated differentially expressed proteins (DEPs) were identified in the PE and NE groups. In addition, 110 differentially expressed metabolites (DMs) were up-regulated and 256 were down-regulated in the PE group. An integrated analysis showed 5667 significant correlations between the metabolites and proteins and 109 common pathways in the proteome and metabolome. The proteins were mostly associated with the mechanistic target of rapamycin (mTOR) pathway, longevity regulation, autophagy, and the pyrimidine and purine metabolism pathways. The metabolites were primarily enriched in amino acid and lipid metabolisms. These results demonstrated the differences in the PE and NE groups at two omics levels and will be useful for the E. sinensis industry.

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Transcriptome and proteome reveal abnormal spermatozoa in precocious Chinese mitten crab, Eriocheir sinensis

Cited by 3 publications

References 37 publications

Comparative Proteomics Elucidates the Potential Mechanism of Sperm Capacitation of Chinese Mitten Crabs (Eriocheir sinensis)

Comparative Proteomics Elucidates the Potential Mechanism of Sperm Capacitation of Chinese Mitten Crabs (Eriocheir sinensis)

Sperm acrosomal released proteome reveals MDH and VDAC3 from mitochondria are involved in acrosome formation during spermatogenesis in Eriocheir sinensis

Integrate Analysis of Eyestalk Proteome and Metabolome in Precocious and Formal Juvenile Female Eriocheir sinensis

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