Transcriptome changes of blue-green algae, Arthrospira sp. in response to sulfate stress

Kumaresan, Venkatesh; Nizam, Faizal; Ravichandran, Gayathri; Viswanathan, Kasi; Palanisamy, R.; Bhatt, Prasanth; Arasu, Mariadhas Valan; Al-Dhabi, Naïf Abdullah; Kundabala, M; Arockiaraj, Jesu

doi:10.1016/j.algal.2017.01.012

Cited by 50 publications

(24 citation statements)

References 43 publications

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“…ROS production was more (20%) in THP-1 and L-6 at lowest concentration (10 µM) [26] which is comparatively higher than NL13, however in NL13 the scavenging activity was high at higher concentration (250 µM). Sannasimuthu et al [20] showed scavenging activity even at lower concentration (12.5 µM) of spirulina peptide, but in our case the peptide function in a dose dependent manner on leucocytes.…”

Section: Cytotoxicity Assay On Human Whole Blood and Intracellular Rocontrasting

confidence: 67%

“…In accordance with the statement, our peptide NL13 also contained leucine (position 3 and 13) and valine (position 9) which predicted its potential antioxidant properties. Further Wattanasiritham et al [20] reported that along with leucine and valine, alanine exhibit a higher antioxidant activity. It is noted that in our peptide sequence position 10 and 11 was occupied with alanine, position 3 and 13 was occupied with leucine and position 9 by valine.…”

Section: Peptide Characterisationmentioning

confidence: 96%

“…The inoculum of A. platensis strain used in this study was isolated from Potheri Lake (12.825527° N 80.039606° E) Tamil Nadu, India. It was grown in 1 l flasks containing modified Zarrouk's medium (Cogne) at temperature 30 °C, pH 9.5 with a 12 L/12 D cycle [20]. The cells grown in Zarrouk medium was served as control.…”

Section: Growth Analysis and Collection Of A Platensis Cellsmentioning

confidence: 99%

“…A Sequence ApAHc was obtained from the transcriptome of A. platensis which was cultured under sulphur deprived state and was constructed earlier using Illumina NextSeq 500 technology [20]. The obtained ApAHc cDNA sequence was presented to the European Nucleotide Archive (EMBL Nucleotide Sequence Database) under ExPASy Translate (web.expasy.org) tool and received the protein sequence.…”

Section: Identification and Sequence Analysis Of Apahcmentioning

confidence: 99%

“…The cDNA sequence ApAHc obtained from the earlier constructed transcriptome of A. platensis [20] File Fig. 1).…”

Section: Analysis Of Apahc Whole Sequencementioning

confidence: 99%

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Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

et al. 2020

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An antioxidant molecule namely, adenosyl homocysteinase (AHc) was identified from the earlier constructed transcriptome database of Spirulina, where it was cultured in a sulphur deprived condition. From the AHc protein, a small peptide NL13 was identified using bioinformatics tools and was predicted to have antioxidant property. Further, the peptide was synthesised and its antioxidant mechanism was addressed at molecular level. NL13 was subjected to various antioxidant assays including DPPH assay, HARS assay, SARS Assay, NO assay and ABTS assay, where NL13 exhibited significant (P < 0.05) potential antioxidant activity compared to its antioxidant control, Trolox. Cytotoxicity was performed on Human whole blood and the cell viability was performed on VERO fibroblast cells. In both assays, it was found that NL13 did not exhibit any cytotoxic effect towards the cells. Further, the intracellular ROS was performed on Multimode reader followed by imaging on fluorescence microscope which showed scavenging activity even at lower concentration of NL13 (31.2 µM). An effective wound healing property of NL13 on VERO cells was confirmed by analysing the cell migration rate at two different time intervals (24 and 48 h). Overall, the study shows that NL13 peptide scavenges the intracellular oxidative stress. Keywords Cyanobacteria • Adenosyl homocysteinase • Wound healing • Fibroblast cells • Spirulina Abbreviations AHc Adenosyl homocysteinase ROS Reactive oxygen species Ap Arthrospira platensis TEAE Trolox equivalent antioxidant capacity HRS Hydroxyl radical scavenging VERO Fibroblast cells Electronic supplementary material The online version of this article (

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Section: Cytotoxicity Assay On Human Whole Blood and Intracellular Rocontrasting

confidence: 67%

Section: Peptide Characterisationmentioning

confidence: 96%

Section: Growth Analysis and Collection Of A Platensis Cellsmentioning

confidence: 99%

Section: Identification and Sequence Analysis Of Apahcmentioning

confidence: 99%

“…The cDNA sequence ApAHc obtained from the earlier constructed transcriptome of A. platensis [20] File Fig. 1).…”

Section: Analysis Of Apahc Whole Sequencementioning

confidence: 99%

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Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

et al. 2020

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NV14 from serine O‐acetyltransferase of cyanobacteria influences the antioxidant enzymes in vitro cells, gene expression against H₂O₂ and other responses in vivo zebrafish larval model

Velayutham

Ojha

Issac

et al. 2021

Cell Biology International

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In this study, we have identified a novel peptide NV14 with antioxidative functions from serine O‐acetyltransferase (SAT) of Artrospira platensis (Ap). The full sequence of ApSAT and its derived NV14 peptide “NVRIGAGSVVLRDV” (141–154) was characterized using bioinformatics tools. To address the transcriptional activity of ApSAT in response to induce generic oxidative stress, the spirulina culture was exposed to H2O2 (10 mM). The ApSAT expression was studied using RT‐PCR across various time points and it was found that the expression of the ApSAT was significantly upregulated on Day 15. The in vitro cytotoxicity assay against NV14 was performed in human dermal fibroblast cells and human blood leukocytes. Results showed that NV14 treatment was non‐cytotoxic to the cells. Besides, in vivo treatment of NV14 in zebrafish larvae did not exhibit the signs of developmental toxicity. Further, the in vitro antioxidant assays enhanced the activity of the antioxidant enzymes, such as SOD and CAT, due to NV14 treatment; and also significantly reduced the MDA levels, while increasing the superoxide radical and H2O2 scavenging activity. The expression of antioxidant enzyme genes glutathione peroxidase, γ‐glutamyl cysteine synthase, and glutathione S‐transferase were found to be upregulated in the NV14 peptide pretreated zebrafish larvae when induced with generic oxidative stress, H2O2. Overall, the study showed that NV14 peptide possessed potent antioxidant properties, which were demonstrated over both in vitro and in vivo assays. NV14 enhanced the expression of antioxidant enzyme genes at the molecular level, thereby modulating and reversing the cellular antioxidant balance disrupted due to the H2O2‐induced oxidative stress.

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Cyanobacterial Stress and Its Omics Perspective

Kharwar

Mukherjee

Kumar³

et al. 2023

Cyanobacterial Biotechnology in the 21st Century

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Transcriptome changes of blue-green algae, Arthrospira sp. in response to sulfate stress

Cited by 50 publications

References 43 publications

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

NV14 from serine O‐acetyltransferase of cyanobacteria influences the antioxidant enzymes in vitro cells, gene expression against H₂O₂ and other responses in vivo zebrafish larval model

Cyanobacterial Stress and Its Omics Perspective

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Transcriptome changes of blue-green algae, Arthrospira sp. in response to sulfate stress

Cited by 50 publications

References 43 publications

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

NV14 from serine O‐acetyltransferase of cyanobacteria influences the antioxidant enzymes in vitro cells, gene expression against H2O2 and other responses in vivo zebrafish larval model

Cyanobacterial Stress and Its Omics Perspective

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NV14 from serine O‐acetyltransferase of cyanobacteria influences the antioxidant enzymes in vitro cells, gene expression against H₂O₂ and other responses in vivo zebrafish larval model