Transcriptome characterization of human gingival mesenchymal and periodontal ligament stem cells in response to electronic-cigarettes

Shen, Yongjun; Liu, Chao; Yang, Tao; Tang, Ying; Shen, Yihang; Gu, Yongchun

doi:10.1016/j.envpol.2023.121307

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…In contrast, the levels of nicotine found in the saliva of users of smokeless tobacco products have been shown to reach cytotoxic thresholds [28]. Despite this, the injurious consequences of conventional tobacco smoking function as essential immune system components, releasing cytokines in response to pathogenic exposure, such as those found in CS or eCV [17][18][19]. The metabolic function of hPDL can significantly impact the success of systematic periodontal therapy [24,25].…”

Section: Apoptosis and Necrosismentioning

confidence: 99%

“…Since many of the molecules contained in both conventional cigarette smoke and Electronic Nicotine Delivery Systems (ENDS) end up in the oral cavity, their levels increase in saliva relative to blood levels. They appear relevant among the molecules suspected to represent a potential threat to the regenerative potential of hPDL and possibly aggravate periodontitis [17][18][19]. However, to date, no studies have directly compared the effects of direct exposure of human periodontal ligament cells (hPDL) to conventional cigarette smoke, electronic cigarette vape, and the aerosol from tobacco "heat-notburn" devices in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic impact of nicotine products on periodontal ligament cells

Wiesmann-Imilowski,

Becker,

Gielisch

et al. 2024

Clin Oral Invest

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Objectives The primary objective of this in vitro experiment was an assessment of proliferative capacity, metabolic activity, and potential cellular detriment of human periodontal ligament cells (hPDL) exposed to cigarette smoke (CS), electronic cigarette vapor (eCV), and heated tobacco product aerosol (HTP), or air (control). Materials and methods Using a CAD/CAM-designed exposition chamber, hPDL were exposed to CS, eCV, HTP, or air (control) based on the Health Canada Intense Smoking Regime. Cell proliferation, metabolic activity, and cellular detriment were assessed at various time points. Results Compared to the control, hPDL exposed to CS exhibited significantly decreased cell numbers at all time points. HTP exposure led to reduced cell numbers 48 h and 72 h post-exposure, while eCV-exposed cells showed no significant decrease. The metabolic activity of eCV-treated hPDL was slightly reduced at 7 h but recovered at 24 h and 48 h. In contrast, CS-treated cells exhibited significantly decreased metabolic activity at 24 h and 48 h, and HTP-exposed cells showed a significant decrease after 48 h. Flow cytometry indicated both apoptotic and necrotic cell death following CS exposure, with necrotic cell death being more pronounced. Conclusions eCV and HTP demonstrated comparatively reduced detrimental effects on hPDL compared to CS. Clinical relevance : The findings suggest that conventional cigarette smoke poses a substantial risk to periodontal health by significantly impairing cell proliferation and metabolic activity. However, alternatives such as eCV and HTP may offer a comparatively reduced risk.

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Section: Apoptosis and Necrosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxic impact of nicotine products on periodontal ligament cells

Wiesmann-Imilowski,

Becker,

Gielisch

et al. 2024

Clin Oral Invest

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“…Moreover, MSCs derived from gingiva (GMSCs) have characteristics similar to those of PDLSCs in periodontal tissues, both of which exhibit high potential for osteogenic differentiation ( SHEN et al, 2023 ). When GMSCs were implanted into an animal model of mandibular defects, they repaired the mandible and promoted new bone formation ( WANG et al, 2011 ).…”

Section: Introduction Of Stem Cells In the Jawmentioning

confidence: 99%

Interaction between immuno-stem dual lineages in jaw bone formation and injury repair

Liu,

Luo,

2024

Front. Cell Dev. Biol.

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The jawbone, a unique structure in the human body, undergoes faster remodeling than other bones due to the presence of stem cells and its distinct immune microenvironment. Long-term exposure of jawbones to an oral environment rich in microbes results in a complex immune balance, as shown by the higher proportion of activated macrophage in the jaw. Stem cells derived from the jawbone have a higher propensity to differentiate into osteoblasts than those derived from other bones. The unique immune microenvironment of the jaw also promotes osteogenic differentiation of jaw stem cells. Here, we summarize the various types of stem cells and immune cells involved in jawbone reconstruction. We describe the mechanism relationship between immune cells and stem cells, including through the production of inflammatory bodies, secretion of cytokines, activation of signaling pathways, etc. In addition, we also comb out cellular interaction of immune cells and stem cells within the jaw under jaw development, homeostasis maintenance and pathological conditions. This review aims to eclucidate the uniqueness of jawbone in the context of stem cell within immune microenvironment, hopefully advancing clinical regeneration of the jawbone.

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What is the impact of e-cigarettes on periodontal stem cells as revealed by transcriptomic analyses?

Mourão,

Shibli

2023

Evid Based Dent

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Transcriptome characterization of human gingival mesenchymal and periodontal ligament stem cells in response to electronic-cigarettes

Cited by 4 publications

References 48 publications

Cytotoxic impact of nicotine products on periodontal ligament cells

Cytotoxic impact of nicotine products on periodontal ligament cells

Interaction between immuno-stem dual lineages in jaw bone formation and injury repair

What is the impact of e-cigarettes on periodontal stem cells as revealed by transcriptomic analyses?

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