Transcriptome Profiles of Leaves and Roots of Goldenrain Tree (Koelreuteria paniculata Laxm.) in Response to Cadmium Stress

He, Qihao; Zhou, Tao; Sun, Jia; Wang, Ping; Yang, Chunping; Bai, Lei; Liu, Zhiming

doi:10.3390/ijerph182212046

Cited by 14 publications

(8 citation statements)

References 45 publications

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“…A growing body of research indicates that TFs are important regulators of the plant response to abiotic stress. In particular, the WRKY, MYB, bHLH, ERF, ZIP, and C2H2 TFs have been implicated in the Cd stress response (Tokumoto et al, 2019;He et al, 2021). We found that the ERF, MYB, C2H2, NAC, WRKY, and G2-like TFs were highly responsive to Cd in the roots and aerial tissues of both uninoculated and "CD2"-inoculated plants (Figure 6), suggesting that these TFs represent an innate Cd stress-resistance mechanism in B. rapa.…”

Section: Discussionmentioning

confidence: 83%

Stenotrophomonas strain CD2 reduces cadmium accumulation in Brassica rapa L.

Fan,

Yuan,

Peng

et al. 2024

Front. Sustain. Food Syst.

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IntroductionCadmium (Cd) is a highly toxic heavy metal which contaminates agricultural soils and is easily absorbed by plants. Brassica rapa L. is one of the most popular vegetables in China and is known to accumulate Cd in its roots and aerial tissues.MethodsA highly Cd-resistant bacterium (‘CD2’) was isolated and identified. Its ability to immobilize Cd(II) in medium was studied. Strain CD2 were added into Cd-polluted soil to ameliorate Cd accumulation in B. rapa. The underlying mechanisms of ‘CD2’ to reduce Cd accumulation in B. rapa. were analyzed by transcriptomics.Results and discussionStrain CD2 was classified as belonging to the genus Stenotrophomonas. Strain CD2 was found to be able to remove 0.1 mmol/L Cd(II) after 36 h by intracellular sequestration and by producing biofilm, exopolysaccharide, and H2S. When applied to Cd-contaminated soil, ‘CD2’ significantly increased the content of nonbioavailable Cd by 212.70%. Furthermore, ‘CD2’-inoculated B. rapa exhibited a 51.16% decrease in the Cd content of roots and a 55.56% decrease in the Cd content of aerial tissues. Transcriptome analysis identified 424 differentially expressed genes (DEGs) in the roots and 501 DEGs in the aerial tissues of uninoculated Cd-exposed plants. By comparison, 1107 DEGs were identified in the roots and 1721 DEGs were identified in the aerial tissues of ‘CD2’-inoculated Cd-exposed plants. In both treatment groups, genes related to vacuolar sequestration were upregulated, resulting in inhibited Cd transport. In addition, both catalase and glutathione transferase were induced in uninoculated plants, while the oxidative stress-related genes CPK and RBOH belonged to ‘plant-pathogen interactions’ were upregulated in ‘CD2’-inoculated plants. Moreover, inoculation with ‘CD2’ resulted in the enrichment of phenylpropane metabolism; cutin, suberine, and wax biosynthesis; and the AP2, Dof, WOX, Trihelix, B3, EIL, and M-type_MADS transcription factors; as well as the downregulation of zinc transporters and blue copper proteins. All of these changes likely contributed to the reduced Cd accumulation in ‘CD2’-inoculated B. rapa. The results of this study suggest that Stenotrophomonas sp. CD2 may prove to be a useful inoculant to prevent Cd accumulation in B. rapa.

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Section: Discussionmentioning

confidence: 83%

Stenotrophomonas strain CD2 reduces cadmium accumulation in Brassica rapa L.

Fan,

Yuan,

Peng

et al. 2024

Front. Sustain. Food Syst.

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“…These ndings highlight the MTP genes' interspeci c functional variety and spatial expression in HM stress responses. Numerous transcriptome studies on HM stress have shown this behavior 40,41 .…”

Section: The Seven-branch Model May Elucidatebjmtpfunctional Diversitymentioning

confidence: 96%

Genome-wide exploration of MTP gene family in mustard (Brassica juncea L.): evolution and expression patterns during heavy metal stress

You,

Sheng,

Jiang

et al. 2024

Preprint

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Members of the Metal Tolerance Protein (MTP) family are critical in mediating the transport and tolerance of divalent metal cations. Despite their significance, little is known about the MTP genes in mustard (Brassica juncea), particularly in relation to how they react to HM stress. In our study, we identified MTP gene sets in Brassica rapa (17 genes), Brassica nigra (18 genes), and B. juncea (33 genes) using the HMMER tool (Cation_efflux; PF01545) and BLAST analysis. Then, for the 33 BjMTPs, we carried out a detailed bioinformatics analysis covering the physicochemical properties, phylogenetic relationships, conserved motifs, protein structures, collinearity, spatiotemporal RNA-seq expression, GO enrichment, and expression profiling under six HM stresses (Mn2+, Fe2+, Zn2+, Cd2+, Sb3+, and Pb2+). According to the findings of physicochemical characteristics and phylogenetic tree, the allopolyploid B. juncea’s MTP genes were inherited from its progenitors, B. rapa and B. nigra, with minimal gene loss during polyploidization. The BjMTP gene family exhibited conserved motifs, promoter elements, and expression patterns that aligned with seven evolutionary branches (G1, G4-G9, and G12). Further, by co-expression analysis, the core and gene-specific expression modules of BjMTPs under six HM stresses were found. The HM treatments exhibited consistently upregulated of BjA04.MTP4, BjA09.MTP10, and BjB01.MTP5 genes, indicating their critical roles in enhancing HM tolerance in B. juncea. These discoveries may contribute to a genetic improvement in B. juncea's HM tolerance, which would facilitate the remediation of HM-contaminated areas.

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“…Arabidopsis thaliana plants overexpressing tomato metallocarboxypeptidase inhibitor (TCMP-1) displayed a lower amount of Cd accumulation in shoots and an increased expression of heavy metal-associated isoprenylated plant protein (AtHIPP26) in comparison with wild-type plants (Manara et al 2020). Few researchers indicated that different transcription factors, including C2H2, MYB, WRKY, and bZIP family members, respond to Cd stress (He et al 2021). Moreover, two basic helix-loop-helix (bHLH) transcription factors (bHLH29 and bHLH38) were different between two wheat genotypes with contrasting Cd accumulation (Sabella et al 2021).…”

Section: Introductionmentioning

confidence: 99%

The impact of cadmium stress on the ascorbate-glutathione pathway and ascorbate regeneration in tea plants

WANG¹,

LIU²,

CHEN³

et al. 2023

Biologia plant.

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Ascorbic acid (AsA) and glutathione (GSH) contribute to defense responses under abiotic stresses. The present study explored the ascorbate-glutathione cycle and ascorbate regeneration under high concentration (30 mM) of cadmium in the tea plant (Camellia sinensis L.). The tea leaves showed speckles and necrosis from the third day of Cd treatment. The content of superoxide anion (O2 .-) and hydrogen peroxide (H2O2) in the leaves were significantly higher until the seventh day after Cd treatment. The content of O2 .and H2O2 were the highest on the fifth day (212.7 and 153.6 % of the control, respectively). The AsA content increased (86.9 %) on the first day after Cd treatment and decreased significantly in the subsequent days, while GSH showed a reverse trend. The enzymatic activity assays showed that dehydroascorbate reductase (DHAR) and glutathione reductase (GR) involved in AsA regeneration were downregulated considerably after Cd foliar application. In contrast, the activities of ascorbate peroxidase (APX) and monodehydroascorbate reductase (MDHAR) increased on the first day and then declined. Reverse-transcription quantitative PCR showed upregulation of glutathione synthetase (CsGSHS), γ-glutamylcysteine synthetase (Csγ-ECS), and CsMDHAR of the AsA regeneration pathway and downregulation of CsDHAR and CsGR. The expressions of GDP-L-galactose phosphorylase (CsGGP), L-galactose-1-phosphate phosphatase (CsGPP), and L-galactono-1,4-lactone dehydrogenase (CsGaILDH) of the L-galactose pathway were also downregulated. The results indicated that AsA, which can respond to Cd stress of plants by increasing antioxidant ability, was consumed to scavenge ROS; moreover, Cd stress inhibited AsA synthesis and regeneration, which made that tea plants suffering severe damage.

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Transcriptome Profiles of Leaves and Roots of Goldenrain Tree (Koelreuteria paniculata Laxm.) in Response to Cadmium Stress

Cited by 14 publications

References 45 publications

Stenotrophomonas strain CD2 reduces cadmium accumulation in Brassica rapa L.

Stenotrophomonas strain CD2 reduces cadmium accumulation in Brassica rapa L.

Genome-wide exploration of MTP gene family in mustard (Brassica juncea L.): evolution and expression patterns during heavy metal stress

The impact of cadmium stress on the ascorbate-glutathione pathway and ascorbate regeneration in tea plants

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