Transcriptome profiling analysis of lung tissue of Chinese soft-shell turtle infected by Trionyx sinensis Hemorrhagic Syndrome Virus

Lyu, Sunjian; Yuan, Xuemei; Zhang, Haiqi; Hang, Xiaoying; Li, Yanli; Shi, Weida; Liu, Li; Yu, Zhe; Yinglei, Wu

doi:10.1016/j.fsi.2019.10.061

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…Pangolin SARS‐like CoV SRR10168377 seems to point to the host range of SARS‐CoV‐2 expanded from pangolin. It is currently known that turtle also acts as a virus reservoir, carrying more than dozens of viruses, including ranavirus (RV), nidovirus (NV), papillomavirus (PV), soft‐shelled turtle iridovirus (STIV), soft‐shelled turtle systemic septicemia spherical virus (STSSSV), tortoise picornavirus (ToPV), and Trionyx sinensis hemorrhagic syndrome virus (TSHSV) 26‐30 . It does not exclude that bat SARS‐like CoV RaTG13 or other SARS‐like CoV infect turtle and expand to humans after evolution.…”

Section: Discussionmentioning

confidence: 99%

“…It is currently known that turtle also acts as a virus reservoir, carrying more than dozens of viruses, including ranavirus (RV), nidovirus papillomavirus (PV), soft-shelled turtle iridovirus (STIV), soft-shelled turtle systemic septicemia spherical virus (STSSSV), tortoise picornavirus (ToPV), and Trionyx sinensis hemorrhagic syndrome virus (TSHSV). [26][27][28][29][30] It does not exclude that bat SARS-like CoV RaTG13 or other SARS-like CoV infect turtle and expand to humans after evolution. This study provides information and possibilities that like snakes and pangolins, turtles (C. picta bellii, C. mydas, and P. sinensis) may also act as the potential intermediate hosts transmitting SARS-CoV-2 to human, although much more needs to be confirmed.…”

Section: Discussionmentioning

confidence: 99%

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Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS‐CoV‐2

Liu

Xiao

Wei

et al. 2020

Journal of Medical Virology

637

607

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From the beginning of 2002 and 2012, severe respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) crossed the species barriers to infect humans, causing thousands of infections and hundreds of deaths, respectively. Currently, a novel coronavirus (SARS-CoV-2), which has become the cause of the outbreak of Coronavirus Disease 2019 (COVID-19), was discovered. Until 18 February 2020, there were 72 533 confirmed COVID-19 cases (including 10 644 severe cases) and 1872 deaths in China. SARS-CoV-2 is spreading among the public and causing substantial burden due to its human-to-human transmission. However, the intermediate host of SARS-CoV-2 is still unclear. Finding the possible intermediate host of SARS-CoV-2is imperative to prevent further spread of the epidemic. In this study, we used systematic comparison and analysis to predict the interaction between the receptor-binding domain (RBD) of coronavirus spike protein and the host receptor, angiotensin-converting enzyme 2 (ACE2). The interaction between the key amino acids of S protein RBD and ACE2 indicated that, other than pangolins and snakes, as previously suggested, turtles (Chrysemys picta bellii, Chelonia mydas, and Pelodiscus sinensis) may act as the potential intermediate hosts transmitting SARS-CoV-2 to humans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS‐CoV‐2

Liu

Xiao

Wei

et al. 2020

Journal of Medical Virology

637

607

View full text Add to dashboard Cite

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“…Meanwhile, studies have shown that the SST has a high nutritional value, since it is high in protein, low in fat, and rich in minerals [ 3 ]. Nonetheless, recurrent incidences of disease have recently posed a severe threat to the sustainable growth and health of the SST aquaculture sector [ 4 , 5 ]. While wild capture fisheries have remained stagnant over the past few decades, a steady expansion of aquaculture has been observed.…”

Section: Introductionmentioning

confidence: 99%

Variations in the Intestinal Microbiota of the Chinese Soft-Shelled Turtle (Trionyx sinensis) between Greenhouse and Pond Aquaculture

Liu,

Zhang,

Xue

et al. 2023

Animals

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The microbial community structure in aquaculture water plays an important role in the intestinal microbial diversity of aquatic animals. The Chinese soft-shelled turtle (SST) (Trionyx sinensis) is an important aquaculture species of high economic value in the Asia-Pacific region. An intuitive understanding of the microbial diversity and abundances of SST aquaculture is crucial for comprehending these ecosystems. Herein, the evolutionary characteristics of the bacterial communities in the SST and its aquaculture water systems were investigated using Illumina MiSeq sequencing. This experiment sampled nine SSTs from a pond outside a greenhouse and was repeated three times. The sequencing results revealed significant differences in the microflora composition at the phylum and genus levels in both the intestine and aquaculture water of the SSTs in the greenhouse and pond aquaculture environments. A total of 1039 genera belonging to 65 phyla were identified. At the phylum level, the relative abundances of Chloroflexi (24%), Acidobacteria (5%), and Nitrospira (3%) were higher in the greenhouse water than in the pond water. The relative abundances of Bacteroidetes (35%), Actinobacteria (8%), and Cyanobacteria (4%) were higher in the pond water than in the greenhouse water. The intestinal microorganisms in the SSTs experienced significant changes after the SSTs were transferred from a greenhouse culture to a pond culture environment for 28 days. After the SSTs were cultured in the ponds, we observed decreases in the relative abundances of Actinobacteria (39% to 25%), Cyanobacteria (24% to 0.8%), Chlorobacteria (9% to 3%), and Firmicutes (5.5% to 0.8%. However, we observed increases in the relative abundances of Bacteroidetes (2% to 35%) and Acidobacteria (0.3% to 25%). These results showed that the bacterial diversity and richness compositions in the intestinal tract and aquaculture water were the same. However, the relative abundances of bacterial communities varied. The results of this study are of great significance in understanding how the environment affects SST cultures. These data may provide valuable instructions for Chinese soft-shelled turtle aquaculture management.

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“…The pathogenicity of the lung transcriptome and the liver proteomics have been investigated in preliminary studies. Notably, RSAD2 was significantly upregulated in mRNA and protein levels after viral infection, suggesting that RSAD2 was involved in antiviral response in T. sinensis [3,4]. Vaccines have achieved outstanding results in preventing aquatic viral diseases.…”

Section: Introductionmentioning

confidence: 99%

The Infection Properties of Trionyx sinensis Hemorrhagic Syndrome Virus and the Antiviral Effect of Curcumin In Vivo

Jiao,

Yao,

Lin

et al. 2023

Animals

Self Cite

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Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is an aquatic arterivirus causing a high mortality rate for T. sinensis (Chinese soft-shelled turtle), but the detailed infection properties of TSHSV are unclear, and no effective treatment is available. In this study, cell culture and histopathology were performed to elucidate the infection properties of TSHSV. Furthermore, the anti-TSHSV and immune-enhancing effects of curcumin were evaluated using survival statistics, qPCR, and tissue immunofluorescence. The results demonstrated that TSHSV could proliferate in the spleen cell line of T. sinensis, leading to cytopathic effects. TSHSV damaged the livers, kidneys, and lungs, characterized by cell disintegration and hyperemia. Curcumin at 250 mg/kg improved the survival of T. sinensis, and significantly reduced the viral load in the spleens, kidneys, and lungs. Moreover, curcumin inhibited the mRNA expression of immune-related genes, RSAD2, IFN-γ, and TNF-α (p < 0.05). In conclusion, these results imply that TSHSV is pathogenic to the spleen cell line, liver, spleen, kidney, and lung of T. sinensis. Curcumin effectively inhibits TSHSV and modulates the immune function of T. sinensis, so it holds promise as a means to prevent TSHSV.

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Transcriptome profiling analysis of lung tissue of Chinese soft-shell turtle infected by Trionyx sinensis Hemorrhagic Syndrome Virus

Cited by 13 publications

References 25 publications

Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS‐CoV‐2

Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS‐CoV‐2

Variations in the Intestinal Microbiota of the Chinese Soft-Shelled Turtle (Trionyx sinensis) between Greenhouse and Pond Aquaculture

The Infection Properties of Trionyx sinensis Hemorrhagic Syndrome Virus and the Antiviral Effect of Curcumin In Vivo

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